Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 25°C
Sample
Lampreys (Petromyzon marinus) Cell (Whole mounted lamprey brains)
Specification
Whole mounted lamprey brains
Permeabilization
Yes - 0.3% Triton X-100
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 6% serum, 0.3% Triton X-100, 0.1M PBS
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Life Technologies Alexa488-conjugated goat anti-ra
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/300
Additional data
Additional Notes
Fixed in 4% PFA in 0.1M PBS, pH 7.4. Pre-absorption of antibody with 100 micrograms of recombinant GST-tagged lamprey synuclein eliminated the fluorescence signal.
PubMed ID
PMID: 22120153: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Jennifer Morgan
Verified customer
提交于 Feb 04 2015