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Neuroscience Neurology process Neurodegenerative disease Parkinson's disease Synuclein

Anti-pan Synuclein抗体(ab6176)

  • Datasheet
  • SDS
Reviews (3) Submit a question References (13)

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Immunocytochemistry/ Immunofluorescence - Anti-pan Synuclein antibody (ab6176)

    Key features and details

    • Rabbit polyclonal to pan Synuclein
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-pan Synuclein抗体
      参阅全部 pan Synuclein 一抗
    • 描述

      兔多克隆抗体to pan Synuclein
    • 宿主

      Rabbit
    • 特异性

      This antibody recognises all 3 forms of the synucleins.
    • 经测试应用

      适用于: ICC/IFmore details
    • 种属反应性

      与反应: Human
      预测可用于: Mouse, Chicken, Cow, Xenopus laevis
    • 免疫原

      Synthetic peptide corresponding to Human pan Synuclein aa 1-100. The conjugate was cross-linked by maleimidocaproyl-N-hydroxy succinimide (MSC) and coupled to diphtheria toxoid.

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      pH: 7
    • Concentration information loading...
    • 纯度

      Whole antiserum
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Parkinson's disease
      • Synuclein
      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Alzheimer's disease
      • Other

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • Recombinant Human Alpha-synuclein protein (1-112aa) (ab48842)
      • Recombinant Human beta Synuclein protein (ab48853)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab6176于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    ICC/IF (1)
    Use a concentration of 1 µg/ml.
    说明
    ICC/IF
    Use a concentration of 1 µg/ml.

    靶标

    • 相关性

      Synucleins are small, soluble proteins expressed primarily in neural tissue and in certain tumors. The family includes three known proteins: alpha, beta and gamma synucleins. Synuclein-alpha is primarily localized at the presynaptic region of axons and it may be involved in the regulation of dopamine release and transport. However, it can form filamentous aggregates that are the major non amyloid component of intracellular inclusions in several neurodegenerative diseases (synucleinopathies). Synuclein-alpha may induce fibrillization of the microtubule-associated protein tau and it reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase 3 activation. Synuclein-alpha is involved in Parkinson's disease, Lewy body dementia, Brain iron accumulation type 1 (NBIA1, also called Hallervorden-Spatz syndrome) and it is a major non-amyloid component of the deposits associated with Alzheimer's disease. Synuclein-beta may be a regulator of the SNCA aggregation process. It protects neurons from staurosporine and 6 hydroxy dopamine (6OHDA)-stimulated caspase activation that also involves p53 and contributes to restore the SNCA anti-apoptotic function abolished by 6OHDA. Gamma synuclein is found primarily in the peripheral nervous system and retina. High levels of expression have been identified in advanced breast carcinomas suggesting a correlation between overexpression of SNCG and breast tumor development.
    • 细胞定位

      Neuronal cytosol and presynaptic terminals
    • 数据库链接

      • Entrez Gene: 281494 Cow
      • Entrez Gene: 6620 Human
      • Entrez Gene: 6622 Human
      • Entrez Gene: 6623 Human
      • Entrez Gene: 104069 Mouse
      • Entrez Gene: 20617 Mouse
      • Entrez Gene: 20618 Mouse
      • Omim: 163890 Human
      • Omim: 602569 Human
      • Omim: 602998 Human
      • SwissProt: Q9NZ50 Cow
      • SwissProt: O76070 Human
      • SwissProt: P37840 Human
      • SwissProt: Q16143 Human
      • SwissProt: Q6FHG5 Human
      • SwissProt: O55042 Mouse
      • SwissProt: Q91ZZ3 Mouse
      • SwissProt: Q9Z0F7 Mouse
      see all
    • 别名

      • BCSG1 antibody
      • beta synuclein antibody
      • Breast cancer specific gene 1 protein antibody
      • Gamma synuclein antibody
      • NACP antibody
      • PARK1 antibody
      • Persyn antibody
      • PRSN antibody
      • SNCA antibody
      • SNCB antibody
      • SNCG antibody
      • SR antibody
      • Synoretin antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-pan Synuclein antibody (ab6176)
      Immunocytochemistry/ Immunofluorescence - Anti-pan Synuclein antibody (ab6176)
      ICC/IF image of ab6176 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6176, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    实验方案

    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (13)

    发表研究结果有使用 ab6176?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab6176 被引用在 13 文献中.

    • McGlinchey RP  et al. C-terminal a-synuclein truncations are linked to cysteine cathepsin activity in Parkinson's disease. J Biol Chem 294:9973-9984 (2019). PubMed: 31092553
    • Mahajani S  et al. Homogenous generation of dopaminergic neurons from multiple hiPSC lines by transient expression of transcription factors. Cell Death Dis 10:898 (2019). PubMed: 31776327
    • Park HJ  et al. The ER retention protein RER1 promotes alpha-synuclein degradation via the proteasome. PLoS One 12:e0184262 (2017). PubMed: 28877262
    • Tenreiro S  et al. Yeast reveals similar molecular mechanisms underlying alpha- and beta-synuclein toxicity. Hum Mol Genet 25:275-90 (2016). ICC/IF ; Human . PubMed: 26586132
    • Sanchez-Guajardo V  et al. a-Synuclein vaccination prevents the accumulation of parkinson disease-like pathologic inclusions in striatum in association with regulatory T cell recruitment in a rat model. J Neuropathol Exp Neurol 72:624-45 (2013). PubMed: 23771222
    View all Publications for this product

    客户评价及客户问答

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    1-3 of 3 Abreviews or Q&A

    Western blot abreview for Anti-pan Synuclein antibody

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Loading amount
    2.5 µg
    Gel Running Conditions
    Reduced Denaturing (10% SDS-PAGE)
    Sample
    Lampreys (Petromyzon marinus) Recombinant protein (GST-tagged synucleins)
    Specification
    GST-tagged synucleins
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Jennifer Morgan

    Verified customer

    提交于 Feb 27 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-pan Synuclein antibody

    Excellent
    Abreviews
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 25°C
    Sample
    Lampreys (Petromyzon marinus) Cell (Whole mounted lamprey brains)
    Specification
    Whole mounted lamprey brains
    Permeabilization
    Yes - 0.3% Triton X-100
    Fixative
    Paraformaldehyde
    Read More

    Jennifer Morgan

    Verified customer

    提交于 Feb 04 2015

    Western blot abreview for Anti-pan Synuclein antibody

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Xenopus laevis Tissue lysate - whole (tectum)
    Loading amount
    20 µg
    Specification
    tectum
    Gel Running Conditions
    Reduced Denaturing (MINI-PROTEAN TGX)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Dr. Wanhua Shen

    Verified customer

    提交于 Apr 09 2012

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