Lamins do not appear to be universally distributed among different cell and tissue types. ab20740 recognizes the karyoskeleton, i.e. the intermediate filament proteins of the nucleus. Reacts with Lamin isotypes of Mr 60-75 kDa (LA, LI, LII, LIII, B1, B2, C). Other cell/tissue types have not been tested.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Used at a dilution of 1/50 for 16 hrs (see Abreview for further information). WB: Use at an assay dependent dilution. Predicted molecular weight: 60-75 kDa. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
ab20740 at a 1/50 dilution staining human U2 OS cells by Immunocytochemistry/Immunofluorescence. The cells were Paraformaldehyde fixed and incubated with the antibody for 16 hours. Bound antibody was detected using an Alexa- Fluor ® 488 conjugated goat anti-mouse IgG.
The antibody was found to stain equally well when used at a dilution of 1/250.
This image is courtesy of an Abreview submitted by Chi Tang on 6 April 2006.
Immunocytochemistry/ Immunofluorescence - Anti-pan Lamin antibody [X67, X167, X233] (ab20740)Image from Mackay DR et al, J Cell Biol. 2010 Nov 29;191(5):923-31. Epub 2010 Nov 22, Fig 1. DOI 10.1083/jcb.201007124
ab20740 staining pan Lamin in HeLa cells by Immunocytochemistry/ Immunofluorescence. Left image shows pan Lamin.Right image shows overlay of pan lamin (green), tubulin (red) and DNA (blue).