概述

  • 产品名称
    Anti-pan Cytokeratin抗体[AE1+AE3],prediluted
    参阅全部 pan Cytokeratin 一抗
  • 描述
    小鼠单克隆抗体[AE1+AE3] to pan Cytokeratin,prediluted
  • 宿主
    Mouse
  • 特异性
    This antibody mixture recognizes acidic and basic subfamilies of cytokeratins. The cocktail of these two antibodies can be used to detect most human epithelia. The acidic cytokeratins have molecular weights of 56.5, 55, 51, 50, 50, 48 46, 45, and 40 kDa. The basic cytokeratins have molecular weights of 65-67, 64, 59, 58, 56 and 52 kDa.
  • 经测试应用
    适用于: ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Chicken, Cow, Cat, Dog, Human, Fish, Zebrafish
  • 免疫原

    Human epidermal cytokeratins

  • 阳性对照
    • Skin or adenocarcinoma.
  • 常规说明

    Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen. 

    This product was changed from ascites to tissue culture supernatant on 8th March 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

性能

应用

Our Abpromise guarantee covers the use of ab961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 19852942
IHC-P Use at an assay dependent concentration. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.

靶标

  • 相关性
    Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
  • 细胞定位
    Cytoplasmic
  • 数据库链接

图片

  • ab961 at a 1/100 dilution staining pan Cytokeratin in human urothelium-like cells in culture by Immunocytochemistry/ Immunofluorescence. Counterstained with DAPI.
  • Immunohistochemical analysis of zebrafish pancreatic tissue, staining pan Cytokeratin with ab961, at 1/500 dilution.

文献

This product has been referenced in:
  • Ramírez R & Marshall SH Identification and isolation of infective filamentous particles in Infectious Salmon Anemia Virus (ISAV). Microb Pathog 117:219-224 (2018). Read more (PubMed: 29454823) »
  • Liu DN  et al. Superior mesenteric artery margin in pancreaticoduodenectomy for pancreatic adenocarcinoma. Oncotarget 8:7766-7776 (2017). Read more (PubMed: 27999192) »
See all 19 Publications for this product

客户评价及客户问答

1-8 of 8 Abreviews or Q&A

Answer

Thank you for contacting us.

I can confirm that the Anti-pan Cytokeratin antibody [AE1+AE3], prediluted (ab961) can be used for applications without further dilutions.

However, every experiment is different and may need optimisation by the user. Some customers seem to get excellent results by diluting this prediluted antibody further. We would recommend to optimise the experiment by trying the undiluted antibody and if possible dilutions found in the literature.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Answer

Merci de nous avoir contactés.

L'anticorps prédilué ab82612 (Anti-Cytokeratin [MNF116]) a une concentration entre 1 et 3 µg/ml.
L'anticorps ab28028 (Anti-Vimentin [Vim 3B4]) est un supernageant, la concentration en anticorps ne peut donc pas être déterminée précisement mais elle est estimée entre 1 et 3 mg/ml.
Malheureusement le laboratoire qui produit l'anticorps ab961 (Anti-pan Cytokeratin antibody [AE1+AE3]) ne désire pas communiquer la concentration de ce produit. Nous sommes désolés de ne pas pouvoir vous fournir cette information, sachez cependant que nous avons au catalogue une référence équivalente, non-prédiluée, ab80826, à 1 mg/ml, https://www.abcam.com/ab80826.




J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

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Answer

Thank you for contacting us.

The antibodies ab6401 and ab11214 are from the same clone [PCK-26], so they are the same antibody with similar binding characteristics. The difference is that ab6401 is not purified and is not conjugated while ab11214 is purified and conjugated to FITC.

The mouse monoclonal [AE1+AE3] to pan Cytokeratin, prediluted, ab961 is already diluted and ready to use for cell and tissue staining right out of the vial. You receive 6 ml of this pre-diluted antibody.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Dog-Kidney)
Specification
Dog-Kidney
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: Rm°C

Jing Ma

Verified customer

提交于 Aug 30 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (tumor)
Specification
tumor
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

提交于 May 01 2009

Answer

Thank you for your enquiry and patience. I was able to obtain the following information from the originators of these antibodies: For ab11213, "this product should recognise cytokeratins 1, 4, 5, 6, 8,10, 13, 18 and 19. Based on literature, respiratory epithelia expressses cytokeratins 5, 6, 7, and 8. Thus I would believe this antibody would prove suitable but I would not have any references available specific for the use of this antibody for rat respiratory epithelia." For ab8794, "we have tested this antibody on skin, colon, pancreas and several other epithelial tissues. We have not tested in respiratory epithelia in rat, although this antibody has broad species reactivity, reacts with mouse, rat bovine, others not tested." I haven't been able to obtain any additional information regarding ab961. If I do, I will pass it on to you. This antibody is a combination of 2 clones and recognizes acidic and basic subfamilies of cytokeratins. The cocktail of these two antibodies can be used to detect most human epithelia. The acidic cytokeratins have molecular weights of 56.5, 55, 51, 50, 50, 48 46, 45, and 40 kDa. The basic cytokeratins have molecular weights of 65-67, 64, 59, 58, 56 and 52. Please contact us again if you have any additional questions.

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Question
Answer

Unfortunately, we do not have more information about this antibody. We will update the on-line datasheet of this product as soon as we get more data. We apologize for any inconvenience caused.

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Question
Answer

According to the datasheet, this antibody recognizes mouse protein. However, we do not have data on mouse kidney tissue particularly. Please have a look at the published references, you may find some more information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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