Key features and details
- Mouse monoclonal [D14HD11] to pan CEACAM
- Reacts with: Human
- Isotype: IgG1
参阅全部 pan CEACAM 一抗
描述小鼠单克隆抗体[D14HD11] to pan CEACAM
特异性Broadly reactive with high affinity (affinity to solid phase bound CEACAM 5: 2 x 1010 L/Mol [Jantscheff P et al.]) with most of the CEACAM molecules (except CEACAM 7 and CEACAM 8) transiently expressed on the cell surface of transfected BOSC23 cells. This antibody can be used to detect CEACAM 1 (BGP/CD66a), CEACAM 3 (CGM1/CD66d), CEACAM 4 (CGM 7), CEACAM 5 and CEACAM 6 (NCA/CD66c) on transfectants by flow cytometry.
Full length native Carcino Embryonic Antigen (partially purified) (Human) from a perchloric acid extract from liver metastases of colonic tumors (Jantscheff P et al.).
常规说明Antibodies produced from cDNA: Conventional technologies usually either generate antibodies against purified proteins, or against synthetic peptides based on amino acid sequences derived from DNA sequence data. Genetic immunization involves introducing the gene in the form of a cDNA directly into an animal which translates this cDNA into protein thus stimulating an immune response against the foreign protein. Although the synthetic peptide approach is comparable in speed, the quality of antibodies generated by genetic immunization is far superior. This is because the protein is made by the immunized animal, utilzing complex cellular mechanisms that allow it to gain a native conformation. Antibodies are then generated against a native protein, such as is found in the blood or tissues of its host species. Membrane-bound or secreted proteins often create problems for conventional antibody technology because in their native form, they are often modified by glycosylation, or in some cases exist as multiple membrane-spanning proteins that are not soluble following isolation or synthesis in recombinant systems. All of these problems are avoided if the immunized animal makes the protein itself. Antibodies generated by genetic immunization have been shown to have binding affinities to the protein in the sub-nanomolar range, which are approximately 100x higher than conventionally developed antibodies and much higher than single chain antibodies. Results confirm published data for much higher avidity of sera generated by genetic immunization as compared with that gained by immunization with a corresponding recombinant protein.
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Concentration information loading...
纯度Protein G purified
相关性CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family. It consists of seven CEACAM (CEACAM 1, CEACAM 3-CEACAM 8) and 11 pregnancy-specific glyco-protein (PSG 1-PSG 11) members. The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/ CEACAM 5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker. The function of CEACAM family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria.
- Antigen CD66 antibody
- Biliary glycoprotein antibody
- Carcinoembryonic antigen related cell adhesion molecule 1 antibody
ab4567 被引用在 4 文献中.
- Johswich KO et al. In vivo adaptation and persistence of Neisseria meningitidis within the nasopharyngeal mucosa. PLoS Pathog 9:e1003509 (2013). IF ; Mouse . PubMed: 23935487
- Patel PC et al. Inside-out signaling promotes dynamic changes in the carcinoembryonic antigen-related cellular adhesion molecule 1 (CEACAM1) oligomeric state to control its cell adhesion properties. J Biol Chem 288:29654-69 (2013). WB ; Human . PubMed: 24005674
- Green LR et al. Cooperative role for tetraspanins in adhesin-mediated attachment of bacterial species to human epithelial cells. Infect Immun 79:2241-9 (2011). PubMed: 21464080
- Haab BB et al. Glycosylation variants of mucins and CEACAMs as candidate biomarkers for the diagnosis of pancreatic cystic neoplasms. Ann Surg 251:937-45 (2010). PubMed: 20395854