Application
Western blot
Sample
Human Cell lysate - whole cell (kidney)
Loading amount
50 µg
Specification
kidney
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Dilution
1/500
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: TBS 5% milk
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit HRP antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Dilution
1/10000
Detection
Detection method
ECL
Exposure
1 minute(s) and 30 second(s)
Bands
Specific: 60 kDa
Additional data
Additional Notes
The different lanes represents 3 different lysate of HEK293
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Nov 07 2007