Application
Western blot
Sample
Mouse Cell lysate - whole cell (macrophage cell lysate)
Loading amount
20 µg
Specification
macrophage cell lysate
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/2000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS-T+5% milk
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 60 kDa
Additional data
Additional Notes
after transferring the equal lysates onto the membrane it was cut in strips.
the strips were incubated with different antibody dilutions. Lane 1, 2 and 3 were incubated with dilutions 1/50, 1/350, 1/2100 respectively.
the strips were incubated with different antibody dilutions. Lane 1, 2 and 3 were incubated with dilutions 1/50, 1/350, 1/2100 respectively.
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提交于 Mar 09 2012