Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Mouse Tissue sections (Day 9.5 embryo)
Specification
Day 9.5 embryo
Permeabilization
Yes - 0.1% saponin
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking buffer: 0.1% saponin, 10% serum
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-rabbit 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/400
Additional data
Additional Notes
IHC fixative concentration: 4% PFA
Worked great for the IHC-Fr as indicated above. Also tried ICC with Nkx2.5+ cells obtained from mouse embryonic stem cells. For this it did not work consistently.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Nikhil Mittal
Verified customer
提交于 Sep 23 2013