Anti-PAK1 + PAK2 + PAK3 (phospho T423)抗体(ab2477)
Key features and details
- Rabbit polyclonal to PAK1 + PAK2 + PAK3 (phospho T423)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PAK1 + PAK2 + PAK3 (phospho T423)抗体
参阅全部 PAK1 + PAK2 + PAK3 一抗 -
描述
兔多克隆抗体to PAK1 + PAK2 + PAK3 (phospho T423) -
宿主
Rabbit -
特异性
This phospho specific polyclonal antibody is specific for phosphorylated Threonine 423 of Human PAK 1 + 2 + 3. Reactivity with non-phosphorylated PAK 1 + 2 + 3 is less than 1% by ELISA. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human PAK1 + PAK2 + PAK3 (phospho T423) conjugated to keyhole limpet haemocyanin. Corresponding to amino acids 413-433 of Human PAK 1, 408-428 of human PAK 2 and 411-431 of human PAK 3.
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常规说明
P21-activated kinase (PAK) is actually a family of serine/threonine protein kinases, members of which are activated by small molecular weight GTPases. The three most common isoforms are PAK 1, PAK 2, and PAK 3 (also known as alpha PAK, gamma PAK, and beta PAK, respectively). These kinases contain numerous regulatory elements that trigger diverse signaling processes such as those initiated by activated GTPases, interaction with Src homology 3 (SH3) domains, and caspase mediated proteolytic cleavage. Autophosphorylation of threonine 423 (threonine 402 for PAK 2 and threonine 421 for PAK 3), catalyzed by Cdc42, is required for activation of PAK.
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性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
This antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. -
Primary antibody说明
P21-activated kinase (PAK) is actually a family of serine/threonine protein kinases, members of which are activated by small molecular weight GTPases. The three most common isoforms are PAK 1, PAK 2, and PAK 3 (also known as alpha PAK, gamma PAK, and beta PAK, respectively). These kinases contain numerous regulatory elements that trigger diverse signaling processes such as those initiated by activated GTPases, interaction with Src homology 3 (SH3) domains, and caspase mediated proteolytic cleavage. Autophosphorylation of threonine 423 (threonine 402 for PAK 2 and threonine 421 for PAK 3), catalyzed by Cdc42, is required for activation of PAK. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/2000. Detects a band of approximately 65 kDa.
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| 说明 |
|---|
|
WB
1/500 - 1/2000. Detects a band of approximately 65 kDa. |
靶标
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相关性
In mammals, there are several identified isoforms of p21 Activated Protein Kinases or PAKs: PAK1 and PAK3 are mostly brain specific, while PAK2 is expressed ubiquitously. Mutations of the gene coding for PAK3 are associated with X linked mental retardation and PAK3 is a key regulator of synapse formation and plasticity in the hippocampus. PAK3 is thought to play a key role in regulation of cell shape and motility as well as cell death. -
细胞定位
Cytoplasm. Recruited to focal adhesions upon activation -
数据库链接
- Entrez Gene: 5058 Human
- Entrez Gene: 5062 Human
- Entrez Gene: 5063 Human
- Omim: 300142 Human
- Omim: 602590 Human
- Omim: 605022 Human
- SwissProt: O75914 Human
- SwissProt: Q13153 Human
see all -
别名
- Alpha PAK antibody
- Beta PAK antibody
- bPAK antibody
see all
图片
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Western blot - Anti-PAK1 + PAK2 + PAK3 (phospho T423) antibody (ab2477)
Western blot probing with PAK1 antibody (ab2477). The major band is at 65 kDa and weaker bands can be detected at 90 and 100 kDa. This picture was kindly submitted by Sue Thomas as part of her review.
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Dot Blot - Anti-PAK1 + PAK2 + PAK3 (phospho T423) antibody (ab2477)Dot Blot analysis labeling phosphorylated and non-phosphorylated forms of the immunizing peptide with ab2477 at 1/1000 dilution, incubated overnight at 4°C. Secondary antibody: Dylight™488 rabbit secondary antibody at 1/40000 dilution for 45 min at room temperature. Block: Blocking Buffer for Fluorescent Western Blotting for 60 min at RT.
Load: 200 ng, 66.7 ng, 22.2 ng, 7.4 ng, or 2.5 ng.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab2477 被引用在 6 文献中.
- Albrecht LV et al. GSK3 Inhibits Macropinocytosis and Lysosomal Activity through the Wnt Destruction Complex Machinery. Cell Rep 32:107973 (2020). PubMed: 32726636
- Singh NK et al. p115 RhoGEF activates the Rac1 GTPase signaling cascade in MCP1 chemokine-induced vascular smooth muscle cell migration and proliferation. J Biol Chem 292:14080-14091 (2017). PubMed: 28655771
- Kumar R et al. A new role for cofilin in retinal neovascularization. J Cell Sci 129:1234-49 (2016). PubMed: 26857814
- Singh NK et al. Disruption of p21-activated kinase 1 gene diminishes atherosclerosis in apolipoprotein E-deficient mice. Nat Commun 6:7450 (2015). PubMed: 26104863
- Liu JS et al. ß-elemene enhances the radiosensitivity of gastric cancer cells by inhibiting Pak1 activation. World J Gastroenterol 21:9945-56 (2015). PubMed: 26379399
- Gadepalli R et al. Novel Role for p21-activated Kinase 2 in Thrombin-induced Monocyte Migration. J Biol Chem 288:30815-31 (2013). WB . PubMed: 24025335