Anti-PADI4 / PAD4抗体(ab26071)
Key features and details
- Goat polyclonal to PADI4 / PAD4
- Suitable for: ICC
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-PADI4 / PAD4抗体
参阅全部 PADI4 / PAD4 一抗 -
描述
山羊多克隆抗体to PADI4 / PAD4 -
宿主
Goat -
经测试应用
适用于: ICCmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human PADI4/ PAD4 aa 1-100 (N terminal).
Database link: NP_036519.2 -
阳性对照
- ICC: HeLa and U2OS cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab26071于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC |
Use a concentration of 10 µg/ml.
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说明 |
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ICC
Use a concentration of 10 µg/ml. |
靶标
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功能
Catalyzes the citrullination/deimination of arginine residues of proteins. Citrullinates histone H3 at 'Arg-8' and/or 'Arg-17' and histone H4 at 'Arg-3', which prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1. -
组织特异性
Expressed in eosinophils and neutrophils, not expressed in peripheral monocytes or lymphocytes. -
疾病相关
Genetic variations in PADI4 are a cause of susceptibility to rheumatoid arthritis (RA) [MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Note=Could have an important role in the pathogenesis of rheumatoid arthritis by increasing citrullination of proteins in rheumatoid arthritis synovial tissues, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context. -
序列相似性
Belongs to the protein arginine deiminase family. -
细胞定位
Cytoplasm. Nucleus. Cytoplasmic granule. Cytoplasmic granules of eosinophils and neutrophils. - Information by UniProt
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数据库链接
- Entrez Gene: 23569 Human
- Omim: 605347 Human
- SwissProt: Q9UM07 Human
- Unigene: 522969 Human
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别名
- EC 3.5.3.15 antibody
- HL 60 PAD antibody
- HL-60 PAD antibody
see all
图片
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Immunocytochemistry analysis of HeLa cells labeling PADI4 / PAD4 antibody with ab26071 at 10 µg/mL (1 hour incubation). Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. Alexa Fluor® 488 2 µg/mL was used as the secondary antibody. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG at 10 µg/mL followed by Alexa Fluor® 488 secondary antibody 2 µg/mL.
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Immunocytochemistry analysis of U2OS cells labeling PADI4 / PAD4 antibody with ab26071 at 10 µg/mL (1 hour incubation). Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. Alexa Fluor® 488 2 µg/mL was used as the secondary antibody. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG at 10 µg/mL followed by Alexa Fluor® 488 secondary antibody 2 µg/mL.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab26071 被引用在 1 文献中.
- Kinloch A et al. Synovial fluid is a site of citrullination of autoantigens in inflammatory arthritis. Arthritis Rheum 58:2287-95 (2008). WB ; Human . PubMed: 18668562