Anti-PADI4 / PAD4抗体(ab50247)
Key features and details
- Rabbit polyclonal to PADI4 / PAD4
- Suitable for: ELISA, IP, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-PADI4 / PAD4抗体
参阅全部 PADI4 / PAD4 一抗 -
描述
兔多克隆抗体to PADI4 / PAD4 -
宿主
Rabbit -
特异性
This antibody reacts specifically with the 74 kDa PADI4 / PAD4 protein. It shows no cross reactivity with the other isoforms. -
经测试应用
适用于: ELISA, IP, ICC/IF, WBmore details -
种属反应性
与反应: Human -
免疫原
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常规说明
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result, we are pleased to offer this antibody in a purified format as of 18th July 2017. The following lots is/are still unpurified and still in stock as of 18th July 2017 - GR316454-5, GR316454-6, GR316454-7, GR316454-8, GR316454-9, GR316454-10. Lot numbers other than GR316454-5, GR316454-6, GR316454-7, GR316454-8, GR316454-9, GR316454-10 will be purified. Please note that the dilutions may need to be adjusted accordingly. Purified antibodies have the advantage of being enriched for the fraction of immunoglobulin that specifically reacts with the target antigen and for having a reduction of serum proteins.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituents: 1.21% Tris, 2% Sucrose, 0.75% Glycine -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab50247于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ELISA |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
|
说明 |
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ELISA
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
靶标
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功能
Catalyzes the citrullination/deimination of arginine residues of proteins. Citrullinates histone H3 at 'Arg-8' and/or 'Arg-17' and histone H4 at 'Arg-3', which prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1. -
组织特异性
Expressed in eosinophils and neutrophils, not expressed in peripheral monocytes or lymphocytes. -
疾病相关
Genetic variations in PADI4 are a cause of susceptibility to rheumatoid arthritis (RA) [MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Note=Could have an important role in the pathogenesis of rheumatoid arthritis by increasing citrullination of proteins in rheumatoid arthritis synovial tissues, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context. -
序列相似性
Belongs to the protein arginine deiminase family. -
细胞定位
Cytoplasm. Nucleus. Cytoplasmic granule. Cytoplasmic granules of eosinophils and neutrophils. - Information by UniProt
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数据库链接
- Entrez Gene: 23569 Human
- Omim: 605347 Human
- SwissProt: Q9UM07 Human
- Unigene: 522969 Human
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别名
- EC 3.5.3.15 antibody
- HL 60 PAD antibody
- HL-60 PAD antibody
see all
数据表及文件
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SDS download
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Datasheet download
文献 (22)
ab50247 被引用在 22 文献中.
- D'Alessio S et al. Acute Hypoxia Alters Extracellular Vesicle Signatures and the Brain Citrullinome of Naked Mole-Rats (Heterocephalus glaber). Int J Mol Sci 23:N/A (2022). PubMed: 35563075
- Inal JM et al. A Pilot Study on Peptidylarginine Deiminases and Protein Deimination in Animal Cancers across Vertebrate Species. Int J Mol Sci 23:N/A (2022). PubMed: 35955829
- El Shikh MEM et al. Follicular dendritic cell differentiation is associated with distinct synovial pathotype signatures in rheumatoid arthritis. Front Med (Lausanne) 9:1013660 (2022). PubMed: 36465908
- Ai P et al. Viral mimetic poly(I:C) induces neutrophil extracellular traps via PAD4 to promote inflammation and thrombosis. Biochem Biophys Res Commun 565:64-71 (2021). PubMed: 34098313
- D'Alessio S et al. Extracellular Vesicle Signatures and Post-Translational Protein Deimination in Purple Sea Urchin (Strongylocentrotus purpuratus) Coelomic Fluid-Novel Insights into Echinodermata Biology. Biology (Basel) 10:N/A (2021). PubMed: 34571743