重组Anti-p75 NGF Receptor抗体[NORI146C] (ab271290)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [NORI146C] to p75 NGF Receptor
- Suitable for: IHC-P, WB, IHC-Fr
- Reacts with: Mouse
Related conjugates and formulations
概述
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产品名称
Anti-p75 NGF Receptor抗体[NORI146C]
参阅全部 p75 NGF Receptor 一抗 -
描述
大鼠单克隆抗体[NORI146C] to p75 NGF Receptor -
宿主
Rat -
经测试应用
适用于: IHC-P, WB, IHC-Frmore details -
种属反应性
与反应: Mouse -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Neuro-2a whole cell lysate. IHC-P: Mouse spleen and liver tissue. IHC-Fr: Mouse spleen tissue.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
NORI146C -
同种型
IgG2a -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry reagents
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab271290于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 0.755 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.755 µg/ml. Predicted molecular weight: 45 kDa.
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IHC-Fr |
Use a concentration of 7.55 µg/ml.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
说明 |
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IHC-P
Use a concentration of 0.755 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.755 µg/ml. Predicted molecular weight: 45 kDa. |
IHC-Fr
Use a concentration of 7.55 µg/ml. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
靶标
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功能
Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. -
序列相似性
Contains 1 death domain.
Contains 4 TNFR-Cys repeats. -
结构域
Death domain is responsible for interaction with RANBP9.
The extracellular domain is responsible for interaction with NTRK1. -
翻译后修饰
N- and O-glycosylated.
O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
Phosphorylated on serine residues. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 18053 Mouse
- SwissProt: Q9Z0W1 Mouse
- Unigene: 283893 Mouse
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别名
- CD271 antibody
- CD271 antigen antibody
- Gp80 LNGFR antibody
see all
图片
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Anti-p75 NGF Receptor antibody [NORI146C] (ab271290) at 0.755 µg/ml + Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate at 20 µg
Secondary
Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution
Predicted band size: 45 kDa
Exposure time: 59 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD271 with ab271290 at 1/1000 dilution (0.755µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the mouse spleen. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling CD271 with ab271290 at 1/1000 dilution (0.755µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the peri-sinusoidal space in the mouse liver. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse spleen tissue labeling CD271 with ab271290 at 1/100 dilution (7.55µg/ml) followed by ab150157 Goat Anti-rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse spleen is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150157 Goat Anti-rat IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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