重组Anti-p75 NGF Receptor抗体[NORI138B] (ab271289)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [NORI138B] to p75 NGF Receptor
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-p75 NGF Receptor抗体[NORI138B]
参阅全部 p75 NGF Receptor 一抗 -
描述
大鼠单克隆抗体[NORI138B] to p75 NGF Receptor -
宿主
Rat -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human brain, Neuro-2a, Mouse brain and Rat brain, PC-12 lysates. IHC-P: Human tonsil tissue.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Ion Exchange Chromatography -
克隆
单克隆 -
克隆编号
NORI138B -
同种型
IgG2a -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab271289于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Predicted molecular weight: 45 kDa.
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IHC-P |
1/1000.
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说明 |
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WB
1/1000. Predicted molecular weight: 45 kDa. |
IHC-P
1/1000. |
靶标
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功能
Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. -
序列相似性
Contains 1 death domain.
Contains 4 TNFR-Cys repeats. -
结构域
Death domain is responsible for interaction with RANBP9.
The extracellular domain is responsible for interaction with NTRK1. -
翻译后修饰
N- and O-glycosylated.
O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
Phosphorylated on serine residues. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 4804 Human
- Entrez Gene: 18053 Mouse
- Entrez Gene: 24596 Rat
- Omim: 162010 Human
- SwissProt: P08138 Human
- SwissProt: Q9Z0W1 Mouse
- SwissProt: P07174 Rat
- Unigene: 415768 Human
see all -
别名
- CD271 antibody
- CD271 antigen antibody
- Gp80 LNGFR antibody
see all
图片
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Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling p75 NGF with ab271289 at 1/1000 dilution followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on the germinal center and basement membrane on the epithelium in the human tonsil. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).
Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0)
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All lanes : Anti-p75 NGF Receptor antibody [NORI138B] (ab271289) at 1/1000 dilution
Lane 1 : Human brain tissue lysate
Lane 2 : Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate
Lane 3 : Mouse brain tissue lysate
Lane 4 : Rat brain tissue lysate
Lane 5 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution
Predicted band size: 45 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: lane 1, 2 : 3 minutes lane 3-5 : 59 seconds
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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