Key features and details
- Mouse monoclonal [NGFR5] to p75 NGF Receptor
- Suitable for: ICC, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG1
Related conjugates and formulations
产品名称Anti-p75 NGF Receptor抗体[NGFR5]
参阅全部 p75 NGF Receptor 一抗
描述小鼠单克隆抗体[NGFR5] to p75 NGF Receptor
经测试应用适用于: ICC, IHC-Pmore details
种属反应性与反应: Rat, Human
预测可用于: Rabbit, Cat, Baboon不与反应: Mouse
Full length native protein (purified) corresponding to p75 NGF Receptor. Purified from A875 melanoma cell line.
表位Amino acids 1 - 160.
- IHC-P: Human testis tissue. ICC/IF: PC-12 cells.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 0.0268% PBS, 0.2% BSA
Concentration information loading...
纯度Protein G purified
- PE Anti-p75 NGF Receptor antibody [NGFR5], prediluted (ab157333)
- APC Anti-p75 NGF Receptor antibody [NGFR5], prediluted (ab176522)
- Anti-p75 NGF Receptor antibody [NGFR5] - BSA and Azide free (ab212682)
- PE/Cy7® Anti-p75 NGF Receptor antibody [NGFR5] (ab234270)
- PerCP/Cy5.5® Anti-p75 NGF Receptor antibody [NGFR5] (ab234446)
功能Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.
序列相似性Contains 1 death domain.
Contains 4 TNFR-Cys repeats.
结构域Death domain is responsible for interaction with RANBP9.
The extracellular domain is responsible for interaction with NTRK1.
翻译后修饰N- and O-glycosylated.
O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
Phosphorylated on serine residues.
- Information by UniProt
- CD271 antibody
- CD271 antigen antibody
- Gp80 LNGFR antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue labelling p75 NGF Receptor with ab3125 at 2 µg/mL.
ICC/IF image of ab3125 stained PC-12 (Rat adrenal gland pheochromocytoma cell line) cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3125, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab3125 被引用在 22 文献中.
- Zhang Y et al. Combination of olfactory ensheathing cells and human umbilical cord mesenchymal stem cell-derived exosomes promotes sciatic nerve regeneration. Neural Regen Res 15:1903-1911 (2020). PubMed: 32246639
- Castle MJ et al. Postmortem Analysis in a Clinical Trial of AAV2-NGF Gene Therapy for Alzheimer's Disease Identifies a Need for Improved Vector Delivery. Hum Gene Ther 31:415-422 (2020). PubMed: 32126838
- Brokhman I et al. Dual embryonic origin of the mammalian enteric nervous system. Dev Biol 445:256-270 (2019). PubMed: 30472119
- Du Y et al. Intracellular Notch1 Signaling in Cancer-Associated Fibroblasts Dictates the Plasticity and Stemness of Melanoma Stem/Initiating Cells. Stem Cells 37:865-875 (2019). PubMed: 30941836
- Rice FL et al. The evolution and multi-molecular properties of NF1 cutaneous neurofibromas originating from C-fiber sensory endings and terminal Schwann cells at normal sites of sensory terminations in the skin. PLoS One 14:e0216527 (2019). PubMed: 31107888