Anti-p75 NGF Receptor抗体[NGFR5] (ab3125)
Key features and details
- Mouse monoclonal [NGFR5] to p75 NGF Receptor
- Suitable for: ICC/IF
- Reacts with: Human, Ferret, Monkey
- Isotype: IgG1
概述
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产品名称
Anti-p75 NGF Receptor抗体[NGFR5]
参阅全部 p75 NGF Receptor 一抗 -
描述
小鼠单克隆抗体[NGFR5] to p75 NGF Receptor -
宿主
Mouse -
经测试应用
适用于: ICC/IFmore details
不适用于: WB -
种属反应性
与反应: Human, Ferret, Monkey
预测可用于: Rabbit, Cat, Baboon不与反应: Mouse, Rat
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免疫原
NGFR from A875 melanoma cells.
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表位
Amino acids 1 - 160. -
阳性对照
- Melanoma.
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常规说明
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituents: 0.0268% PBS, 0.2% BSA -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
NGFR5 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
- PE Anti-p75 NGF Receptor antibody [NGFR5], prediluted (ab157333)
- APC Anti-p75 NGF Receptor antibody [NGFR5], prediluted (ab176522)
- Anti-p75 NGF Receptor antibody [NGFR5] - BSA and Azide free (ab212682)
- PE/Cy7® Anti-p75 NGF Receptor antibody [NGFR5] (ab234270)
- PerCP/Cy5.5® Anti-p75 NGF Receptor antibody [NGFR5] (ab234446)
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Compatible Secondaries
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Isotype control
应用
Our Abpromise guarantee covers the use of ab3125 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
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ICC/IF | Use at an assay dependent concentration. |
靶标
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功能
Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. -
序列相似性
Contains 1 death domain.
Contains 4 TNFR-Cys repeats. -
结构域
Death domain is responsible for interaction with RANBP9.
The extracellular domain is responsible for interaction with NTRK1. -
翻译后修饰
N- and O-glycosylated.
O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
Phosphorylated on serine residues. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 101101519 Cat
- Entrez Gene: 101680416 Ferret
- Entrez Gene: 4804 Human
- Entrez Gene: 100349095 Rabbit
- Omim: 162010 Human
- SwissProt: P08138 Human
- Unigene: 415768 Human
- Unigene: 681726 Human
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别名
- CD271 antibody
- CD271 antigen antibody
- Gp80 LNGFR antibody
see all
图片
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ICC/IF image of ab3125 stained PC-12 (Rat adrenal gland pheochromocytoma cell line) cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3125, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody [NGFR5] (ab3125)This image is courtesy of Hongwei Shao by Abreview.ab3125 staining p75 NGF Receptor in human melanoma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed using Tris EDTA pH 9.0. Samples were then permeabilized using 0.25% Triton X-100, blocked for 1 hour at room temperature and then incubated with ab3125 at a 1/100 dilution for 18 hours at 4°C. The secondary used was an Alexa-Fluor 594 (red) conjugated donkey anti-mouse polyclonal used at a 1/100 dilution. (Green) tumor cells, (blue) DAPI.
数据表及文件
文献 (19)
ab3125 被引用在 19 文献中.
- Du Y et al. Intracellular Notch1 Signaling in Cancer-Associated Fibroblasts Dictates the Plasticity and Stemness of Melanoma Stem/Initiating Cells. Stem Cells 37:865-875 (2019). PubMed: 30941836
- Rice FL et al. The evolution and multi-molecular properties of NF1 cutaneous neurofibromas originating from C-fiber sensory endings and terminal Schwann cells at normal sites of sensory terminations in the skin. PLoS One 14:e0216527 (2019). PubMed: 31107888
- Foerster Y et al. Relevance of Neurotrophin Receptors CD271 and TrkC for Prognosis, Migration, and Proliferation in Head and Neck Squamous Cell Carcinoma. Cells 8:N/A (2019). PubMed: 31569361
- Brokhman I et al. Dual embryonic origin of the mammalian enteric nervous system. Dev Biol 445:256-270 (2019). PubMed: 30472119
- Li Y et al. ROCK Inhibitor Y27632 Induced Morphological Shift and Enhanced Neurite Outgrowth-Promoting Property of Olfactory Ensheathing Cells via YAP-Dependent Up-Regulation of L1-CAM. Front Cell Neurosci 12:489 (2018). PubMed: 30618636