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Anti-p75 NGF Receptor抗体[NGFR5] (ab3125)

  • Datasheet
Reviews (5)Q&A (9)References (19)

Product price, shipping and contact information

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Abpromise

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Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody [NGFR5] (ab3125)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody [NGFR5] (ab3125)

Key features and details

  • Mouse monoclonal [NGFR5] to p75 NGF Receptor
  • Suitable for: ICC/IF
  • Reacts with: Human, Ferret, Monkey
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-p75 NGF Receptor抗体[NGFR5]
    参阅全部 p75 NGF Receptor 一抗
  • 描述

    小鼠单克隆抗体[NGFR5] to p75 NGF Receptor
  • 宿主

    Mouse
  • 经测试应用

    适用于: ICC/IFmore details
    不适用于: WB
  • 种属反应性

    与反应: Human, Ferret, Monkey
    预测可用于: Rabbit, Cat, Baboon不与反应: Mouse, Rat
  • 免疫原

    NGFR from A875 melanoma cells.

  • 表位

    Amino acids 1 - 160.
  • 阳性对照

    • Melanoma.
  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液

    pH: 7.40
    Constituents: 0.0268% PBS, 0.2% BSA
  • Concentration information loading...
  • 纯度

    Protein G purified
  • 克隆

    单克隆
  • 克隆编号

    NGFR5
  • 同种型

    IgG1
  • 轻链类型

    kappa
  • 研究领域

    • Neuroscience
    • Neurology process
    • Growth and Development
    • Neurotrophins
    • Stem Cells
    • Neural Stem Cells
    • Surface Molecules
    • Stem Cells
    • Mesenchymal Stem Cells
    • Surface Molecules

相关产品

  • Alternative Versions

    • PE Anti-p75 NGF Receptor antibody [NGFR5], prediluted (ab157333)
    • APC Anti-p75 NGF Receptor antibody [NGFR5], prediluted (ab176522)
    • Anti-p75 NGF Receptor antibody [NGFR5] - BSA and Azide free (ab212682)
    • PE/Cy7® Anti-p75 NGF Receptor antibody [NGFR5] (ab234270)
    • PerCP/Cy5.5® Anti-p75 NGF Receptor antibody [NGFR5] (ab234446)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

应用

Our Abpromise guarantee covers the use of ab3125 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
  • 应用说明
    Is unsuitable for WB.
  • 靶标

    • 功能

      Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.
    • 序列相似性

      Contains 1 death domain.
      Contains 4 TNFR-Cys repeats.
    • 结构域

      Death domain is responsible for interaction with RANBP9.
      The extracellular domain is responsible for interaction with NTRK1.
    • 翻译后修饰

      N- and O-glycosylated.
      O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
      Phosphorylated on serine residues.
    • 细胞定位

      Membrane.
    • Target information above from: UniProt accession P08138 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 101101519 Cat
      • Entrez Gene: 101680416 Ferret
      • Entrez Gene: 4804 Human
      • Entrez Gene: 100349095 Rabbit
      • Omim: 162010 Human
      • SwissProt: P08138 Human
      • Unigene: 415768 Human
      • Unigene: 681726 Human
      • 别名

        • CD271 antibody
        • CD271 antigen antibody
        • Gp80 LNGFR antibody
        • Gp80-LNGFR antibody
        • Low affinity nerve growth factor receptor antibody
        • Low affinity neurotrophin receptor p75NTR antibody
        • Low-affinity nerve growth factor receptor antibody
        • Nerve growth factor receptor antibody
        • Nerve growth factor receptor TNFR superfamily member 16 antibody
        • NGF receptor antibody
        • Ngfr antibody
        • p75 ICD antibody
        • p75 Neurotrophin receptor antibody
        • p75 NTR antibody
        • p75(NTR) antibody
        • p75NTR antibody
        • TNFR Superfamily Member 16 antibody
        • TNFRSF16 antibody
        • TNR16_HUMAN antibody
        • Tumor necrosis factor receptor superfamily member 16 antibody
        see all

      图片

      • Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody [NGFR5] (ab3125)
        Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody [NGFR5] (ab3125)

        ICC/IF image of ab3125 stained PC-12 (Rat adrenal gland pheochromocytoma cell line) cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3125, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody [NGFR5] (ab3125)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody [NGFR5] (ab3125)This image is courtesy of Hongwei Shao by Abreview.
        ab3125 staining p75 NGF Receptor in human melanoma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
        Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed using Tris EDTA pH 9.0. Samples were then permeabilized using 0.25% Triton X-100, blocked for 1 hour at room temperature and then incubated with ab3125 at a 1/100 dilution for 18 hours at 4°C. The secondary used was an Alexa-Fluor 594 (red) conjugated donkey anti-mouse polyclonal used at a 1/100 dilution. (Green) tumor cells, (blue) DAPI.

        See Abreview

      实验方案

      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      数据表及文件

      • Datasheet
    • 文献 (19)

      发表研究结果有使用 ab3125?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab3125 被引用在 19 文献中.

      • Du Y  et al. Intracellular Notch1 Signaling in Cancer-Associated Fibroblasts Dictates the Plasticity and Stemness of Melanoma Stem/Initiating Cells. Stem Cells 37:865-875 (2019). PubMed: 30941836
      • Rice FL  et al. The evolution and multi-molecular properties of NF1 cutaneous neurofibromas originating from C-fiber sensory endings and terminal Schwann cells at normal sites of sensory terminations in the skin. PLoS One 14:e0216527 (2019). PubMed: 31107888
      • Foerster Y  et al. Relevance of Neurotrophin Receptors CD271 and TrkC for Prognosis, Migration, and Proliferation in Head and Neck Squamous Cell Carcinoma. Cells 8:N/A (2019). PubMed: 31569361
      • Brokhman I  et al. Dual embryonic origin of the mammalian enteric nervous system. Dev Biol 445:256-270 (2019). PubMed: 30472119
      • Li Y  et al. ROCK Inhibitor Y27632 Induced Morphological Shift and Enhanced Neurite Outgrowth-Promoting Property of Olfactory Ensheathing Cells via YAP-Dependent Up-Regulation of L1-CAM. Front Cell Neurosci 12:489 (2018). PubMed: 30618636
      View all Publications for this product

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      1-10 of 14 Abreviews or Q&A

      Immunocytochemistry/ Immunofluorescence abreview for Anti-p75 NGF Receptor antibody [NGFR5]

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Dog Cell (neural precursor)
      Permeabilization
      No
      Specification
      neural precursor
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      提交于 Sep 13 2018

      Immunocytochemistry/ Immunofluorescence abreview for Anti-p75 NGF Receptor antibody [NGFR5]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Pig Cell (Spermatogonial stem cells differentiating into gli)
      Permeabilization
      Yes - 0.1 % Triton-X
      Specification
      Spermatogonial stem cells differentiating into gli
      Blocking step
      BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
      Fixative
      Paraformaldehyde
      Read More

      Dominic Schomberg

      Verified customer

      提交于 Apr 18 2018

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-p75 NGF Receptor antibody [NGFR5]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Bone Marrow)
      Antigen retrieval step
      Enzymatic - Buffer/Enzyme Used: Proteinase K
      Permeabilization
      No
      Specification
      Bone Marrow
      Blocking step
      Serum Free Protein Block (Dako) as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
      Fixative
      10% NBF, 10% Formic Acid Decalcification
      Read More

      Premier Laboratory

      Verified customer

      提交于 Mar 29 2016

      Question

      I was wondering if I could get a sample of two antibodies to test this method once before I purchase a large amount of antibody. The two antibodies for which I would like samples are as follows: Anti-Metallothionein antibody [UC1MT] (ab12228) Anti-p75 NGF Receptor antibody [NGFR5] (ab3125).

      Read More

      Abcam community

      Verified customer

      Asked on Apr 11 2013

      Answer

      We do not have any free samples or small samples of any of our antibodies but both ab12228 and ab3125 will be covered by our guarantee for one year if you plan on using them for flow cytometry with samples that are one of the species listed on the datasheets. (Human, ferret, and monkey are listed on the ab3125 datasheet and human, mouse, rat and several others on the ab12228 datasheet).

      Note that metallothionein is cytoplasmic, so you will not be able to sort live cells. Antibody ab3125 anti-p75 NGF Receptor, on the other hand, recognizes an extracellular epitope, so you should be able to sort live cells.

      Read More

      Abcam Scientific Support

      回复于 Apr 11 2013

      Question

      Bonjour,
      J'ai utilisé le kit ab64261 pour le NGF (6199) et le TrkA (37837).

      pour les photo, nous avons un problème avec notre appareil. Pour le moment cela nous est impossible. C'est pour cela que je voulais vous envoyer les lames.

      Salutations

      Read More

      Abcam community

      Verified customer

      Asked on May 22 2012

      Answer

      Merci pour votre émail. Je suis désolée que nous pouvons par regarder les images du coup. Nous sommes en Angleterre, et je pense que ça va devenir compliqué pour envoyer les lames.

      Je vais chercher pour trouver des images de mon coté et je vous tiens au courant. Est-ce que en attendant, vous pouvez me confirmerles pointssuivants:

      1.)que ces bien des tissus d'origine humaine que vous avez marqué? Ou c'est des tissus d'origine de souris?

      2.) C'est quoi comme sérum que vous avez utilisé pour bloquer les lames?

      3.) A votre avis, le problème vient de quel réactif?

      Je me réjouis de votre réponse. Veuillez s'il vous plait aussi me confirmer comment vous souhaitez procéder.

      Read More

      Abcam Scientific Support

      回复于 May 23 2012

      Question

      Hi there,I am currently working with Cd271 selected MSCs derived from bone marrow. My experiments now require a Cd271 antibody as well as a CD271 primer for qPCR. I cannot see these products on your website and therefore wondering if you still supply these. I was recommended to try this company by acolleaguebut I'm not having much luck finding these on your site.Any information would be greatly appreciated.

      Read More

      Abcam community

      Verified customer

      Asked on Jan 16 2012

      Answer

      Thank you for your inquiry.

      I am pleased to let you know that we currently have a number of CD271 antibodies (24 in total); we sell them under the alternative name 'p75-NGF receptor', such as the rabbit polyclonal antibody ab8874 and the mouse monoclonal ab8877 (as can be seen on each datasheet in the section 'Alternative names'):
      Click here (or use the following: https://www.abcam.com/p75-NGF-Receptor-antibody-ab8874.html).
      Click here (or use the following: https://www.abcam.com/p75-NGF-Receptor-antibody-ME204-ab8877.html).

      These antibodies are guaranteed to work with mouse and human samples (amongst others) and in a number of applications. We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase (www.abcam.com/Abpromise).

      If you are looking for other specifications (e.g. other species or applications), I would be pleased to assist you further in finding the appropriate antibody.

      If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information.

      Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates. To find out more about our Abreview system, please see the following link: https://www.abcam.com/abreviews

      We do not have many qPCR primer in our catalog. We have primer for common controls such as GAPDH (ab83588) available, but CD271/p75-NGF receptor is unfortunately not in this selection.

      I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

      Read More

      Abcam Scientific Support

      回复于 Jan 16 2012

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-p75 NGF Receptor antibody [NGFR5]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Melanoma)
      Specification
      Melanoma
      Fixative
      Formaldehyde
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Tris EDTA pH9.0
      Permeabilization
      Yes - 0.25%Triton X-100
      Blocking step
      DakoCytomation X0909 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
      Read More

      Hongwei Shao

      Verified customer

      提交于 Nov 09 2011

      Question

      Bonjour, J'ai effectué de nouveaux tests en augmentant la concentration. J'ai obtenu un marquage pour NGF et TrkA fort mais avec beaucoup de fond. La p75 est toujours faible, donc je vais la concentré plus. Pour repondre à votre question, j'ai bien reçu les 3 anticorps dans le même colis. Merci pour votre aide Meilleures salutations

      Read More

      Abcam community

      Verified customer

      Asked on Nov 02 2011

      Answer

      Merci pour ces précisions. N'hésitez pas à nous contacter de nouveau si vous rencontrez des difficultés.  

      Read More

      Abcam Scientific Support

      回复于 Nov 02 2011

      Question

      Bonjour, J'ai effectué les tests pour les 3 anticorps avec les paramètres que vous m'aviez conseillé. Je n'ai malheureusement pas de resultats très concluant. Je vais effectuer un test en concentrant plus les anticorps. Est ce que le tissu utilisé pour les les tests est le plus apropriez ? Merci d'avance pour votre aide. Meilleures salutations

      Read More

      Abcam community

      Verified customer

      Asked on Oct 31 2011

      Answer

      Le tissu cérébral humain est en effet un tissu de choix pour l'expression de TrkA, NGF et le récepteur p75 NGF. Avez-vous reçu ces 3 anticorps en même temps (même colis de livraison)?  

      Read More

      Abcam Scientific Support

      回复于 Oct 31 2011

      Question

      Bonjour, nous avons bien reçu vos anticorps, merci. J'ai effectué un premier test qui n'a malheureusement pas fonctionné. Voici les paramètres: NGF (ab 6199): Pas de prétraitement, dilution a 1/500 incubé 30 minute, utilisations du système ENVISION/HRP de Dako pour anticorps rabbit polyclonal, utilisation PBS comme washbuffer. TRKa (ab 37837): Paramètres identique que ci-dessus, dilution à 1/100 P75 (ab 3125): Pré traitement à la chaleur de 3*5 minutes au Micro-ondes à 600w dans tampon citrate a pH 6, dilution à 1/200 incubé pendant 30 minutes, utilisation de système ENVISION/HRP de Dako pour Mouse monoclonal antibody, utiisaion de PBS. J'aimerais savoir si en utilisant de TBS plutôt que du PBS cela fonctionnerait mieux? Et si je dois procéder à un prétraitement pour le NGF et le TrkA ? Le test à été effectué sur du tissus cérébral humain. Merci d'avance pour votre réponse et meilleures salutations.

      Read More

      Abcam community

      Verified customer

      Asked on Oct 12 2011

      Answer

      Merci de nous avoir contactés. Je suis désolé d'apprendre que vous avez eu des difficultés à obtenir de bons résultats avec ab3125, ab6199, ab37837 en IHC-P. Notre politique est d'offrir tout d'abord la meilleure assistance technique. Si nous concluons que le produit que vous avez reçu ne fonctionne pas comme mentionné sur sa fiche technique et que ce produit a été commandé sous 6 mois, nous nous ferons un plaisir de vous offrir un remplacement gratuit ou un avoir. J'aimerais donc vous offrir quelques suggestions afin d'optimiser vos résultats. - Pour les 3 anticorps je recommanderais une incubation sur la nuit à 4°C (ou même à température ambiante) en prenant soin que les échantillons ne soient pas sujet à évaporation. Pour les dilutions des anticorps primaires, se reporter aux fiches techniques. - Pour les 3 anticorps je recommanderais également un démasquage de l'antigène à haute température en utilisant du tampon Tris EDTA pH 9,0. Pourriez-vous me communiquer quelques détails concernant la préparation des échantillons (agent et temps de fixation)? Les anticorps secondaires sont-il compatibles? Sont-ils bons? J'espère que ces informations vous aideront. Si les suggestions ne permettent pas d'améliorer vos résultats, n'hésitez pas à me recontacter pour plus d'assistance.

      Read More

      Abcam Scientific Support

      回复于 Oct 12 2011

      1-10 of 14 Abreviews or Q&A

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