Anti-p75 NGF Receptor抗体- Affinity Purified (ab8875)

概述

  • 产品名称
    Anti-p75 NGF Receptor抗体- Affinity Purified
    参阅全部 p75 NGF Receptor 一抗
  • 描述
    兔多克隆抗体to p75 NGF Receptor - Affinity Purified
  • 宿主
    Rabbit
  • 经测试应用
    适用于: Flow Cyt, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    An extracellular fragment from the mouse p75 receptor (amino acids 43-161).

性能

应用

Our Abpromise guarantee covers the use of ab8875 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.
IHC-P 1/150.

靶标

  • 功能
    Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.
  • 序列相似性
    Contains 1 death domain.
    Contains 4 TNFR-Cys repeats.
  • 结构域
    Death domain is responsible for interaction with RANBP9.
    The extracellular domain is responsible for interaction with NTRK1.
  • 翻译后修饰
    N- and O-glycosylated.
    O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
    Phosphorylated on serine residues.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD271 antibody
    • CD271 antigen antibody
    • Gp80 LNGFR antibody
    • Gp80-LNGFR antibody
    • Low affinity nerve growth factor receptor antibody
    • Low affinity neurotrophin receptor p75NTR antibody
    • Low-affinity nerve growth factor receptor antibody
    • Nerve growth factor receptor antibody
    • Nerve growth factor receptor TNFR superfamily member 16 antibody
    • NGF receptor antibody
    • Ngfr antibody
    • p75 ICD antibody
    • p75 Neurotrophin receptor antibody
    • p75 NTR antibody
    • p75(NTR) antibody
    • p75NTR antibody
    • TNFR Superfamily Member 16 antibody
    • TNFRSF16 antibody
    • TNR16_HUMAN antibody
    • Tumor necrosis factor receptor superfamily member 16 antibody
    see all

图片

  • NG108-15 cells, a fusion of mouse neuroblastoma and rat glioma cells, were used for a FACS analysis. Cells were incubated for one hour with Ab8875 at a 1:100 dilution, and subsequently with an anti-rabbit IgG conjugated to FITC. A 96% shift was seen relative to control cells treated with secondary antibody only. (note; yellow = control, red = result). 

  • ab8875 staining p75 NGF Receptor in the COS1 fibroblast cell line from Monkey kidney by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% in PBS and blocked with 1% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/300) for 16 hour at 4°C. An Alexa Fluor®594-conjugated Goat anti-rabbit polyclonal(1/500) was used as the secondary antibody.

    See Abreview

文献

This product has been referenced in:
  • Chang YT  et al. DNA methyltransferase inhibition restores erythropoietin production in fibrotic murine kidneys. J Clin Invest 126:721-31 (2016). Read more (PubMed: 26731474) »
  • Motohashi T  et al. Gene array analysis of neural crest cells identifies transcription factors necessary for direct conversion of embryonic fibroblasts into neural crest cells. Biol Open 5:311-22 (2016). Flow Cyt . Read more (PubMed: 26873953) »

See all 9 Publications for this product

客户评价及客户问答

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: RT°C
Sample
Rat Tissue sections (Plantar Skin)
Specification
Plantar Skin
Permeabilization
Yes - 10% Tx
Fixative
Paraformaldehyde
Username

Mr. Matthew Thakur

Verified customer

提交于 Oct 21 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH3T3)
Specification
NIH3T3
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Mar 22 2013

Application
Immunocytochemistry/ Immunofluorescence
Sample
Monkey Cell (COS1 cell line)
Specification
COS1 cell line
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 11 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Loading amount
30 µg
Specification
HEK293
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Nov 21 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Embryonic lung)
Loading amount
30 µg
Specification
Embryonic lung
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Nov 20 2012

Thank you very much for your call yesterday.

I've checked with the lab, and ab8875 is provided in PBS only, so it is suitable to use with the EasyLink conjugation kits. I had another look at the recommended dimensions, and you are correct, th...

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I am very pleased to hear you would like to accept our offer and test ab8875 inbovine. This code will give you: 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview for bovine and include this code in the &l...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for contacting Abcam regarding ab8875.


I have confirmed with the laboratory that our in-house quality control quantifies proteinby way of BCA. This method produced the 1.4 mg/ml concentration issued to this specific lot. Retesti...

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Thank you for your enquiry and your patience. (1) It is dissolved in PBS and it does not contain any Tris or glycine or any preservatives. (2) 4.2 ug are used for each sample, which would have 1x10^6 cells. We did not directly label the primary ...

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1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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