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Our Abpromise guarantee covers the use of ab8875 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use at an assay dependent concentration.|
NG108-15 cells, a fusion of mouse neuroblastoma and rat glioma cells, were used for a FACS analysis. Cells were incubated for one hour with Ab8875 at a 1:100 dilution, and subsequently with an anti-rabbit IgG conjugated to FITC. A 96% shift was seen relative to control cells treated with secondary antibody only. (note; yellow = control, red = result).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"