重组Anti-p73抗体[EPR18409(T)(MIX)] - BSA and Azide free (ab250999)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18409(T)(MIX)] to p73 - BSA and Azide free
- Suitable for: WB, IP, Flow Cyt (Intra), IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-p73抗体[EPR18409(T)(MIX)] - BSA and Azide free
参阅全部 p73 一抗 -
描述
兔单克隆抗体[EPR18409(T)(MIX)] to p73 - BSA and Azide free -
宿主
Rabbit -
特异性
The immunogen used for this product shares 76% homology with p63. Cross-reactivity with this protein has not been confirmed experimentally.
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经测试应用
适用于: WB, IP, Flow Cyt (Intra), IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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常规说明
ab250999 is the carrier-free version of ab189896.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading...
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克隆
单克隆 -
克隆编号
EPR18409(T)(MIX) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab250999于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
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IP |
Use at an assay dependent concentration.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
Use at an assay dependent concentration.
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说明 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa). |
IP
Use at an assay dependent concentration. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
Use at an assay dependent concentration. |
靶标
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功能
Participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein. -
组织特异性
Expressed in striatal neurons of patients with Huntington disease (at protein level). Brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus and pancreas. Highly expressed in fetal tissue. -
序列相似性
Belongs to the p53 family.
Contains 1 SAM (sterile alpha motif) domain. -
结构域
Possesses an acidic transactivation domain, a central DNA binding domain and a C-terminal oligomerization domain that binds to the ABL tyrosine kinase SH3 domain.
The WW-binding motif mediates interaction with WWOX. -
翻译后修饰
Isoform alpha (but not isoform beta) is sumoylated on Lys-627, which potentiates proteasomal degradation but does not affect transcriptional activity.
Higher levels of phosphorylation seen in the brain from patients with Huntington disease.
Ubiquitinated; leading to its degradation by the proteasome. -
细胞定位
Nucleus. Accumulates in the nucleus in response to DNA damage. - Information by UniProt
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数据库链接
- Entrez Gene: 7161 Human
- Entrez Gene: 22062 Mouse
- Entrez Gene: 362675 Rat
- Omim: 601990 Human
- SwissProt: O15350 Human
- SwissProt: Q9JJP2 Mouse
- Unigene: 697294 Human
- Unigene: 706990 Human
see all -
别名
- p53 like transcription factor antibody
- p53 related protein antibody
- p53-like transcription factor antibody
see all
图片
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Anti-p73 antibody [EPR18409(T)(MIX)] (ab189896) at 1/5000 dilution + Recombinant fragment of human p73 protein at 0.005 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 70 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsThis data was developed using ab189896, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Recombinant fragment of human p73 protein contains aa380-636 with His-Tag®.
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All lanes : Anti-p73 antibody [EPR18409(T)(MIX)] (ab189896) at 1/5000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 4 : WEHI-3 (Mouse leukemia cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 70 kDa
Observed band size: 70 kDa
Exposure time: 3 minutesThis data was developed using ab189896, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-p73 antibody [EPR18409(T)(MIX)] (ab189896) at 1/5000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/50000 dilution
Predicted band size: 70 kDa
Observed band size: 70 kDa
Exposure time: 3 minutesThis data was developed using ab189896, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-p73 antibody [EPR18409(T)(MIX)] (ab189896) at 1/5000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Mouse spleen lysate
Lane 5 : Rat brain lysate
Lane 6 : Rat spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 70 kDa
Observed band size: 70 kDa
Exposure time: 3 minutesThis data was developed using ab189896, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-p73 antibody [EPR18409(T)(MIX)] (ab189896) at 1/5000 dilution
Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 70 kDa
Observed band size: 70 kDa
Exposure time: 5 secondsThis data was developed using ab189896, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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This data was developed using ab189896, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling p73 alpha+beta with ab189896 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Human cerebral cortex. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
This data was developed using ab189896, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling p73 alpha+beta with ab189896 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic and weak nucleus staining on Human spermatogonial cells is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
This data was developed using ab189896, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling p73 alpha+beta with ab189896 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus and cytoplasmic staining on Human glioma is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
This data was developed using ab189896, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling p73 alpha+beta with ab189896 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus and cytoplasmic staining on Human gastric carcinoma is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
This data was developed using ab189896, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling p73 alpha+beta with ab189896 at 1/1000 dilution, followed by Goat anti-rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).Confocal image showing nuclear and cytoplasm staining on MCF7 cell line. The nuclear counterstain is DAPI (blue).Tubulin is detected with Anti-alpha Tubulin antibody-Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).The negative controls are as follows:--ve control 1: ab189896 at 1/1000 dilution followed by ab150120 at 1/1000 dilution.-ve control 2: ab7291at 1/1000 dilution followed by ab150077 at 1/1000 dilution. -
This data was developed using ab189896, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling p73 alpha+beta with ab189896 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).Confocal image showing cytoplasmic and weakly nuclear staining on HeLa cell line. The nuclear counterstain is DAPI (blue).Tubulin is detected with Anti-alpha Tubulin antibody - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).The negative controls are as follows:--ve control 1: ab189896 at 1/1000 dilution followed by ab150120 at 1/1000 dilution.-ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.
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This data was developed using ab189896, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling p73 alpha+beta with ab189896 at 1/300 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.
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This data was developed using ab189896, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed K562 (Human chronic myelogenous leukemia cell line from bone marrow) cells labeling p73 alpha+beta with ab189896 at 1/300 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.
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This data was developed using ab189896, the same antibody clone in a different buffer formulation.p73 alpha+beta was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab189896 at 1/80 dilution.Western blot was performed from the immunoprecipitate using ab189896 at 1/1000 dilution.Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.Lane 1: HeLa whole cell lysate 10ug (Input).Lane 2: ab189896 IP in HeLa whole cell lysate.Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab189896 in HeLa whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 10 seconds.
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This data was developed using ab189896, the same antibody clone in a different buffer formulation.p73 alpha+beta was immunoprecipitated from 1mg of MCF7 (Human breast adenocarcinoma cell line) whole cell lysate with ab189896 at 1/80 dilution.Western blot was performed from the immunoprecipitate using ab189896 at 1/1000 dilution.Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.Lane 1: MCF7 whole cell lysate 10ug (Input).Lane 2: ab189896 IP in MCF7 whole cell lysate.Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab189896 in MCF7 whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 10 seconds.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
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