• 产品名称

    参阅全部 p63 一抗
  • 描述

    小鼠单克隆抗体[4A4] to p63
  • 宿主

  • 经测试应用

    适用于: ICC/IF, IHC-P, IHC-Frmore details
  • 种属反应性

    与反应: Mouse, Rat, Human, Turtle
    预测可用于: Dog
  • 免疫原

    Recombinant fragment corresponding to Human p63 aa 1-205. This antibody is raised against the N-terminus of ∆N p63 isoform of mouse protein.
    Database link: Q9H3D4-2

  • 阳性对照

    • Normal prostate.
  • 常规说明

    Please note that clone [BC4A4] and clone [4A4] are identical.

    This product was changed from ascites to tissue culture supernatant on [14/07/17]. The following lots are from ascites and are still in stock as of [14/07/17] – [GR323232, GR293168, GR301030]. Lot numbers higher than [GR323232] will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.



Our Abpromise guarantee covers the use of ab735 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 15800938
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

See Sanderson et al and Huange et al. or the Abreview 22860.

Alternatively, steam tissue sections for 45-60 minutes. Allow solution to cool for 10 minutes then wash in distilled water.


IHC-Fr Use at an assay dependent concentration.


  • 功能

    Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes. Involved in Notch signaling by probably inducing JAG1 and JAG2. Plays a role in the regulation of epithelial morphogenesis. The ratio of DeltaN-type and TA*-type isoforms may govern the maintenance of epithelial stem cell compartments and regulate the initiation of epithelial stratification from the undifferentiated embryonal ectoderm. Required for limb formation from the apical ectodermal ridge.
  • 组织特异性

    Widely expressed, notably in heart, kidney, placenta, prostate, skeletal muscle, testis and thymus, although the precise isoform varies according to tissue type. Progenitor cell layers of skin, breast, eye and prostate express high levels of DeltaN-type isoforms. Isoform 10 is predominantly expressed in skin squamous cell carcinomas, but not in normal skin tissues.
  • 疾病相关

    Defects in TP63 are the cause of acro-dermato-ungual-lacrimal-tooth syndrome (ADULT syndrome) [MIM:103285]; a form of ectodermal dysplasia. Ectodermal dysplasias (EDs) constitute a heterogeneous group of developmental disorders affecting tissues of ectodermal origin. EDs are characterized by abnormal development of two or more ectodermal structures such as hair, teeth, nails and sweat glands, with or without any additional clinical sign. Each combination of clinical features represents a different type of ectodermal dysplasia. ADULT syndrome involves ectrodactyly, syndactyly, finger- and toenail dysplasia, hypoplastic breasts and nipples, intensive freckling, lacrimal duct atresia, frontal alopecia, primary hypodontia, and loss of permanent teeth. ADULT differs significantly from EEC3 syndrome by the absence of facial clefting.
    Defects in TP63 are the cause of ankyloblepharon-ectodermal defects-cleft lip/palate (AEC) [MIM:106260]. AEC is an autosomal dominant condition characterized by congenital ectodermal dysplasia with coarse, wiry, sparse hair, dystrophic nails, slight hypohidrosis, scalp infections, ankyloblepharon filiform adnatum, maxillary hypoplasia, hypodontia and cleft lip/palate.
    Defects in TP63 are the cause of ectrodactyly-ectodermal dysplasia-cleft lip/palate syndrome type 3 (EEC3) [MIM:604292]. EEC3 is an autosomal dominant syndrome characterized by ectrodactyly of hands and feet, ectodermal dysplasia and facial clefting.
    Defects in TP63 are the cause of split-hand/foot malformation type 4 (SHFM4) [MIM:605289]. Split-hand/split-foot malformation is a limb malformation involving the central rays of the autopod and presenting with syndactyly, median clefts of the hands and feet, and aplasia and/or hypoplasia of the phalanges, metacarpals, and metatarsals. There is restricted overlap between the mutational spectra of EEC3 and SHFM4.
    Defects in TP63 are the cause of limb-mammary syndrome (LMS) [MIM:603543]. LMS is characterized by ectrodactyly, cleft palate and mammary-gland abnormalities.
    Note=Defects in TP63 are a cause of cervical, colon, head and neck, lung and ovarian cancers.
    Defects in TP63 are a cause of ectodermal dysplasia Rapp-Hodgkin type (EDRH) [MIM:129400]; also called Rapp-Hodgkin syndrome or anhidrotic ectodermal dysplasia with cleft lip/palate. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EDRH is characterized by the combination of anhidrotic ectodermal dysplasia, cleft lip, and cleft palate. The clinical syndrome is comprised of a characteristic facies (narrow nose and small mouth), wiry, slow-growing, and uncombable hair, sparse eyelashes and eyebrows, obstructed lacrimal puncta/epiphora, bilateral stenosis of external auditory canals, microsomia, hypodontia, cone-shaped incisors, enamel hypoplasia, dystrophic nails, and cleft lip/cleft palate.
    Defects in TP63 are the cause of non-syndromic orofacial cleft type 8 (OFC8) [MIM:129400]. Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum.
  • 序列相似性

    Belongs to the p53 family.
    Contains 1 SAM (sterile alpha motif) domain.
  • 结构域

    The transactivation inhibitory domain (TID) can interact with, and inhibit the activity of the N-terminal transcriptional activation domain of TA*-type isoforms.
  • 翻译后修饰

    May be sumoylated.
    Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.
  • 细胞定位

  • Information by UniProt
  • 数据库链接

  • 别名

    • AIS antibody
    • Amplified in squamous cell carcinoma antibody
    • B(p51A) antibody
    • B(p51B) antibody
    • Chronic ulcerative stomatitis protein antibody
    • CUSP antibody
    • DN p63 alpha 1 antibody
    • DNp63 antibody
    • EEC3 antibody
    • id:ibd3516 antibody
    • Keratinocyte transcription factor antibody
    • Keratinocyte transcription factor KET antibody
    • KET antibody
    • LMS antibody
    • MGC115972 antibody
    • MGC192897 antibody
    • NBP antibody
    • OFC8 antibody
    • OTTHUMP00000209732 antibody
    • OTTHUMP00000209733 antibody
    • OTTHUMP00000209734 antibody
    • OTTHUMP00000209735 antibody
    • OTTHUMP00000209737 antibody
    • OTTHUMP00000209738 antibody
    • OTTHUMP00000209739 antibody
    • OTTHUMP00000209740 antibody
    • OTTHUMP00000209741 antibody
    • OTTHUMP00000209742 antibody
    • OTTHUMP00000209743 antibody
    • OTTHUMP00000209744 antibody
    • p40 antibody
    • p51 antibody
    • P51/P63 antibody
    • p53-related protein p63 antibody
    • p53CP antibody
    • p63 antibody
    • P63_HUMAN antibody
    • p73H antibody
    • p73L antibody
    • RHS antibody
    • SHFM4 antibody
    • TAp63alpha antibody
    • TP53CP antibody
    • TP53L antibody
    • TP63 antibody
    • TP73L antibody
    • Transformation related protein 63 antibody
    • Transformation-related protein 63 antibody
    • Trp53rp1 antibody
    • Trp63 antibody
    • Tumor protein 63 antibody
    • Tumor protein p53-competing protein antibody
    • Tumor protein p53-like antibody
    • Tumor protein p63 antibody
    • Tumor protein p63 deltaN isoform delta antibody
    • Tumor protein p73 antibody
    • Tumor protein p73-like antibody
    see all


  • Immunohistochemical analysis of prostate tissue labelling p63 with ab735.

  • Anti-p53 antibody (ab735) staining p63 in murine mammary gland tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer. Samples were then blocked with 4% serum for 1 hour at 22°C followed by incubation with the primary antibody at a 1/40 dilution for 15 hours at 4°C. A Biotin-conjugated anti-mouse polyclonal was used as secondary antibody at a 1/250 dilution. Note positive nuclear staining of the basal cell population.

    See Abreview


This product has been referenced in:

  • Yang BY  et al. Porous Se@SiO2 nanosphere-coated catheter accelerates prostatic urethra wound healing by modulating macrophage polarization through reactive oxygen species-NF-?B pathway inhibition. Acta Biomater 88:392-405 (2019). Read more (PubMed: 30753941) »
  • Zhong B  et al. A p53-stabilizing agent, CP-31398, induces p21 expression with increased G2/M phase through the YY1 transcription factor in esophageal carcinoma defective of the p53 pathway. Am J Cancer Res 9:79-93 (2019). Read more (PubMed: 30755813) »
See all 43 Publications for this product


1-9 of 9 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (Prostate)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Blocking step
Dako Protein Block, serum-free as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 21°C

Abcam user community

Verified customer

提交于 Jun 18 2018

Human Cell lysate - nuclear (Epidermal keratinocytes)
Negative control
Negative control loci: Myoglobin exon 2 region, Chr11 intergenic region
Epidermal keratinocytes
Detection step
Real-time PCR
Cross-linking (X-ChIP)
Duration of cross-linking step: 0 second(s)
Positive control
Positive control loci: p21 promoter, BPAG1, MiR-205, IRF6

Jo Zhou

Verified customer

提交于 Dec 30 2015

Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Human Cell lysate - nuclear (Normal Human Keratinocyte)
Normal Human Keratinocyte
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

提交于 Mar 30 2015


There are many isoforms of different sizes. Twelve are listed at the UniProt entry for human p63.


The molecular weights listed there (you will need to scroll far down the page) are calculated from the amino acid sequences, and do not take into account post-translational modification that might add or subtract weight. The MW of these precursors range from about 77 to 45 kDa.

The 4A4 clone is expected to recognize all known isoforms. Depending on the samples, blots may appear like the blot stained by ab124762, which apparently also recognizes several isoforms, some of which are more prevalent in some cell lines than other, assuming all bands in the blot image are p63:


Read More


For ab735, anti-p63 (currently unpublished), we do not have any western blot data but we did at one point offer this same clone, 4A4 (referred to on the ab735 datasheet as BC4A4), as a different catalogue item, ab3239. That product was used in the following publication for western blotting:

Craig AL et al. DeltaNp63 transcriptionally regulates ATM to control p53 Serine-15 phosphorylation. Mol Cancer 9:195 (2010). PubMed: 20663147


I suggest trying it at 1/500 to start.

For ab11575, anti-laminin, we have several positive reviews from customers who have used the antibody for western blotting. Links to the reviews are here:


The dilution that was used in each case was 1/1000.

Read More
Immunohistochemistry (Frozen sections)
Human Tissue sections (Breast)
Blocking step
Serum as blocking agent for 5 minute(s) · Concentration: 10% · Temperature: 25°C

Dr. Rene Villadsen

Verified customer

提交于 Jul 13 2011

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Turtle Tissue sections (normal skin)
normal skin
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 1mM EDTA pH8 30min
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 27°C

Abcam user community

Verified customer

提交于 Apr 19 2011

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Rat Tissue sections (normal skin)
normal skin
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 1mM EDTA pH8 30min
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 27°C

Abcam user community

Verified customer

提交于 Apr 15 2011

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Mouse Tissue sections (Mammary gland)
Mammary gland
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 22°C

Abcam user community

Verified customer

提交于 Jan 28 2011

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