Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Non-reduced Denaturing (4% gel)
Sample
Fruit fly (Drosophila melanogaster) Cell lysate - whole cell (DmD8 and Kc167 cell lines)
Specification
DmD8 and Kc167 cell lines
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking buffer
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: Goat Anti-Rabbit IgG (H+L)-HRP Conjugate
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL
Bands
Specific: 55 kDa Non-specific: 90, 140, 160 kDa
Exposure
1 minute(s) and 0 second(s)
Positive control
Lane 1 - Kc167 cell lysate, Lane 2 - DmD8 cell lysate
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kat Millen
Verified customer
提交于 Jul 17 2013