Application
Immunoprecipitation
Immuno-precipitation step
Other - Dynabeads Protein G
Sample
Human Cell lysate - whole cell (HCT116 human colon carcinoma cell line)
Specification
HCT116 human colon carcinoma cell line
Treatment
15 ´M etoposide for 24 hrs
Total protein in input
7e+006 cells
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: lysis buffer
Dilution
1/150
Western Blot antibody
Name
Abcam antibody:Donkey Anti-Mouse IgG H&L (Alexa Fluor 750) preadsorbed
Dilution
1/5000
Additional data
Additional Notes
HCT116 p53-/- cells were used as negative control. Immunodetection was performed with a non-abcam anti p53 antibody and a fluorescence labeled secondary antibody from abcam. Dynabeads Protein G were cross linked with 6.5 ´g antibody per mg beads.
The antibody works specifically, but the amount of immunoprecipitated p53 could be higher.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Oliver Maddocks
Verified customer
提交于 Jun 22 2014