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Cell Biology Cell Cycle Cell Cycle Inhibitors p53
使用敲除细胞株进行验证

Anti-p53抗体[PAb 240] (ab26)

  • Datasheet
  • SDS
Reviews (52)Q&A (31)References (212)

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Western blot - Anti-p53 antibody [PAb 240] (ab26)
  • Immunocytochemistry/ Immunofluorescence - Anti-p53 antibody [PAb 240] (ab26)
  • Western blot - Anti-p53 antibody [PAb 240] (ab26)
  • Western blot - Anti-p53 antibody [PAb 240] (ab26)
  • Western blot - Anti-p53 antibody [PAb 240] (ab26)
  • Western blot - Anti-p53 antibody [PAb 240] (ab26)
  • Western blot - Anti-p53 antibody [PAb 240] (ab26)

Key features and details

  • Mouse monoclonal [PAb 240] to p53
  • Suitable for: ICC/IF, WB
  • Knockout validated
  • Reacts with: Mouse, Human
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-p53抗体[PAb 240]
    参阅全部 p53 一抗
  • 描述

    小鼠单克隆抗体[PAb 240] to p53
  • 宿主

    Mouse
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Mouse
    Human
    See all applications and species data
  • 免疫原

    Recombinant fragment. Consists of amino acids 14-389 of p53 fused to ß-galactosidase.

  • 表位

    The epitope has been mapped to between amino acids 212 and 217 on human p53 (PMID: 1376364).
  • 阳性对照

    • WB: HCT116, A431, HeLa, NIH/3T3, MCF7, MDA231. ICC/IF: A431.
  • 常规说明

    ab26 has been knockout validated in Western blot. The expected band was seen in wild type HCT116 cells treated with the DNA damaging agent irinotecan and no band was seen in TP53 knockout HCT116 cells.

    We recommend using 3% milk as the blocking agent for Western blot.

    Please note that expression of target protein may be very low without stimulation/treatment (e.g. DNA damaging agent).

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • 纯度

    IgG fraction
  • 克隆

    单克隆
  • 克隆编号

    PAb 240
  • 骨髓瘤

    Sp2
  • 同种型

    IgG1
  • 轻链类型

    kappa
  • 研究领域

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • p53
    • Cell Biology
    • Apoptosis
    • Intracellular
    • p53 Pathway
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • p53
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Tumor Suppressors
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Cycle Inhibitors
    • p53
    • Cancer
    • Cell cycle
    • Cell cycle inhibitors
    • p53 pathway
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • p53 pathway

相关产品

  • Alternative Versions

    • Anti-p53 antibody [PAb 240] - BSA and Azide free (ab176243)
  • Assay kits

    • p53 Transcription Factor Assay Kit (Colorimetric) (ab207225)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant Human p53 protein (ab43615)
  • Related Products

    • Neurokinin A, Tachykinin peptide (ab120185)
    • Andrographolide, anti-inflammatory agent (ab120636)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab26 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
ICC/IF
Human
WB
Mouse
Human
All applications
Rat
Cow
Dog
Monkey
Chinese hamster
Syrian hamster
应用 Ab评论 说明
ICC/IF (24)
Use a concentration of 0.5 - 1 µg/ml.
WB (18)
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).

Please note that expression of target protein may be very low without stimulation/treatment (e.g. DNA damaging agent). We recommend using 3% milk as the blocking agent for Western blot.

说明
ICC/IF
Use a concentration of 0.5 - 1 µg/ml.
WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).

Please note that expression of target protein may be very low without stimulation/treatment (e.g. DNA damaging agent). We recommend using 3% milk as the blocking agent for Western blot.

靶标

  • 功能

    Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis.
  • 组织特异性

    Ubiquitous. Isoforms are expressed in a wide range of normal tissues but in a tissue-dependent manner. Isoform 2 is expressed in most normal tissues but is not detected in brain, lung, prostate, muscle, fetal brain, spinal cord and fetal liver. Isoform 3 is expressed in most normal tissues but is not detected in lung, spleen, testis, fetal brain, spinal cord and fetal liver. Isoform 7 is expressed in most normal tissues but is not detected in prostate, uterus, skeletal muscle and breast. Isoform 8 is detected only in colon, bone marrow, testis, fetal brain and intestine. Isoform 9 is expressed in most normal tissues but is not detected in brain, heart, lung, fetal liver, salivary gland, breast or intestine.
  • 疾病相关

    Note=TP53 is found in increased amounts in a wide variety of transformed cells. TP53 is frequently mutated or inactivated in about 60% of cancers. TP53 defects are found in Barrett metaplasia a condition in which the normally stratified squamous epithelium of the lower esophagus is replaced by a metaplastic columnar epithelium. The condition develops as a complication in approximately 10% of patients with chronic gastroesophageal reflux disease and predisposes to the development of esophageal adenocarcinoma.
    Defects in TP53 are a cause of esophageal cancer (ESCR) [MIM:133239].
    Defects in TP53 are a cause of Li-Fraumeni syndrome (LFS) [MIM:151623]. LFS is an autosomal dominant familial cancer syndrome that in its classic form is defined by the existence of a proband affected by a sarcoma before 45 years with a first degree relative affected by any tumor before 45 years and another first degree relative with any tumor before 45 years or a sarcoma at any age. Other clinical definitions for LFS have been proposed (PubMed:8118819 and PubMed:8718514) and called Li-Fraumeni like syndrome (LFL). In these families affected relatives develop a diverse set of malignancies at unusually early ages. Four types of cancers account for 80% of tumors occurring in TP53 germline mutation carriers: breast cancers, soft tissue and bone sarcomas, brain tumors (astrocytomas) and adrenocortical carcinomas. Less frequent tumors include choroid plexus carcinoma or papilloma before the age of 15, rhabdomyosarcoma before the age of 5, leukemia, Wilms tumor, malignant phyllodes tumor, colorectal and gastric cancers.
    Defects in TP53 are involved in head and neck squamous cell carcinomas (HNSCC) [MIM:275355]; also known as squamous cell carcinoma of the head and neck.
    Defects in TP53 are a cause of lung cancer (LNCR) [MIM:211980].
    Defects in TP53 are a cause of choroid plexus papilloma (CPLPA) [MIM:260500]. Choroid plexus papilloma is a slow-growing benign tumor of the choroid plexus that often invades the leptomeninges. In children it is usually in a lateral ventricle but in adults it is more often in the fourth ventricle. Hydrocephalus is common, either from obstruction or from tumor secretion of cerebrospinal fluid. If it undergoes malignant transformation it is called a choroid plexus carcinoma. Primary choroid plexus tumors are rare and usually occur in early childhood.
    Defects in TP53 are a cause of adrenocortical carcinoma (ADCC) [MIM:202300]. ADCC is a rare childhood tumor of the adrenal cortex. It occurs with increased frequency in patients with the Beckwith-Wiedemann syndrome and is a component tumor in Li-Fraumeni syndrome.
  • 序列相似性

    Belongs to the p53 family.
  • 结构域

    The nuclear export signal acts as a transcriptional repression domain. The TADI and TADII motifs (residues 17 to 25 and 48 to 56) correspond both to 9aaTAD motifs which are transactivation domains present in a large number of yeast and animal transcription factors.
  • 翻译后修饰

    Acetylated. Acetylation of Lys-382 by CREBBP enhances transcriptional activity. Deacetylation of Lys-382 by SIRT1 impairs its ability to induce proapoptotic program and modulate cell senescence.
    Phosphorylation on Ser residues mediates transcriptional activation. Phosphorylated by HIPK1 (By similarity). Phosphorylation at Ser-9 by HIPK4 increases repression activity on BIRC5 promoter. Phosphorylated on Thr-18 by VRK1. Phosphorylated on Ser-20 by CHEK2 in response to DNA damage, which prevents ubiquitination by MDM2. Phosphorylated on Thr-55 by TAF1, which promotes MDM2-mediated degradation. Phosphorylated on Ser-46 by HIPK2 upon UV irradiation. Phosphorylation on Ser-46 is required for acetylation by CREBBP. Phosphorylated on Ser-392 following UV but not gamma irradiation. Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylated on Ser-15 upon ultraviolet irradiation; which is enhanced by interaction with BANP.
    Dephosphorylated by PP2A-PPP2R5C holoenzyme at Thr-55. SV40 small T antigen inhibits the dephosphorylation by the AC form of PP2A.
    May be O-glycosylated in the C-terminal basic region. Studied in EB-1 cell line.
    Ubiquitinated by MDM2 and SYVN1, which leads to proteasomal degradation. Ubiquitinated by RFWD3, which works in cooperation with MDM2 and may catalyze the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. Ubiquitinated by MKRN1 at Lys-291 and Lys-292, which leads to proteasomal degradation. Deubiquitinated by USP10, leading to its stabilization. Ubiquitinated by TRIM24, which leads to proteasomal degradation. Ubiquitination by TOPORS induces degradation. Deubiquitination by USP7, leading to stabilization. Isoform 4 is monoubiquitinated in an MDM2-independent manner.
    Monomethylated at Lys-372 by SETD7, leading to stabilization and increased transcriptional activation. Monomethylated at Lys-370 by SMYD2, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity. Lys-372 monomethylation prevents interaction with SMYD2 and subsequent monomethylation at Lys-370. Dimethylated at Lys-373 by EHMT1 and EHMT2. Monomethylated at Lys-382 by SETD8, promoting interaction with L3MBTL1 and leading to repress transcriptional activity. Demethylation of dimethylated Lys-370 by KDM1A prevents interaction with TP53BP1 and represses TP53-mediated transcriptional activation.
    Sumoylated by SUMO1.
  • 细胞定位

    Cytoplasm; Cytoplasm. Nucleus. Nucleus > PML body. Endoplasmic reticulum. Interaction with BANP promotes nuclear localization. Recruited into PML bodies together with CHEK2; Nucleus. Cytoplasm. Localized in both nucleus and cytoplasm in most cells. In some cells, forms foci in the nucleus that are different from nucleoli; Nucleus. Cytoplasm. Localized in the nucleus in most cells but found in the cytoplasm in some cells; Nucleus. Cytoplasm. Localized mainly in the nucleus with minor staining in the cytoplasm; Nucleus. Cytoplasm. Predominantly nuclear but localizes to the cytoplasm when expressed with isoform 4 and Nucleus. Cytoplasm. Predominantly nuclear but translocates to the cytoplasm following cell stress.
  • Target information above from: UniProt accession P04637 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 281542 Cow
    • Entrez Gene: 403869 Dog
    • Entrez Gene: 7157 Human
    • Entrez Gene: 22059 Mouse
    • Entrez Gene: 24842 Rat
    • Omim: 191170 Human
    • SwissProt: P67939 Cow
    • SwissProt: Q29537 Dog
    • SwissProt: P04637 Human
    • SwissProt: P02340 Mouse
    • SwissProt: P10361 Rat
    • Unigene: 654481 Human
    • Unigene: 222 Mouse
    • Unigene: 54443 Rat
    see all
  • 别名

    • Antigen NY-CO-13 antibody
    • BCC7 antibody
    • Cellular tumor antigen p53 antibody
    • FLJ92943 antibody
    • LFS1 antibody
    • Mutant tumor protein 53 antibody
    • p53 antibody
    • p53 tumor suppressor antibody
    • P53_HUMAN antibody
    • Phosphoprotein p53 antibody
    • Tp53 antibody
    • Transformation related protein 53 antibody
    • TRP53 antibody
    • tumor antigen p55 antibody
    • Tumor protein 53 antibody
    • Tumor protein p53 antibody
    • Tumor suppressor p53 antibody
    see all

图片

  • Western blot - Anti-p53 antibody [PAb 240] (ab26)
    Western blot - Anti-p53 antibody [PAb 240] (ab26)
    All lanes : Anti-p53 antibody [PAb 240] (ab26) at 5 µg/ml

    Lane 1 : Wild-type HCT116 cell lysate at 30 µg
    Lane 2 : Wild-type HCT116 + irinotecan (10 µM, 24 hours) cell lysate at 30 µg
    Lane 3 : p53 knockout HCT116 cell lysate at 30 µg
    Lane 4 : p53 knockout HCT116 + irinotecan (10 µM, 24 hours) cell lysate at 30 µg
    Lane 5 : A431 cell lysate (positive control) at 20 µg
    Lane 6 : Saos-2 cell lysate (negative control) at 20 µg

    Performed under reducing conditions.

    Predicted band size: 53 kDa
    Observed band size: 53 kDa



    Lanes 1-6: Merged (red and green) signal.


    Ab26 was shown to specifically react with p53 in wild type HCT116 cells treated with irinotecan. No band was observed in p53 knockout HCT116 cells. Wild-type and p53 knockout samples, positive and negative controls were subjected to SDS-PAGE. Ab26 and ab181602(loading control to GAPDH) were diluted 5 μg/mL and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging.

    Wild-type and p53 knockout HCT116 cell lysates were kindly provided by a collaborator.

  • Immunocytochemistry/ Immunofluorescence - Anti-p53 antibody [PAb 240] (ab26)
    Immunocytochemistry/ Immunofluorescence - Anti-p53 antibody [PAb 240] (ab26)

    ab26 stained in A431 cells. Cells were fixed with 4% paraformaldehyde (10min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with ab26 at 1µg/ml and ab6046 (Rabbit polyclonal to beta tubulin) at 1ug/ml overnight at +4°C. The secondary antibodies were ab150177 (colored green) used at 1 ug/ml and ab150087 (pseudo-colored red) used at 2ug/ml for 1hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Western blot - Anti-p53 antibody [PAb 240] (ab26)
    Western blot - Anti-p53 antibody [PAb 240] (ab26)
    Anti-p53 antibody [PAb 240] (ab26) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 53 kDa
    Observed band size: 53 kDa


    Exposure time: 8 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab26 overnight at 4°C. Antibody binding was detected using an anti-mouse HRP secondary antibody, and visualised using ECL development solution ab133406

  • Western blot - Anti-p53 antibody [PAb 240] (ab26)
    Western blot - Anti-p53 antibody [PAb 240] (ab26)
    All lanes : Anti-p53 antibody [PAb 240] (ab26) at 5 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Hela Whole Cell Lysate - Bleomycin Treated (40U/ml)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 53 kDa


    Exposure time: 4 minutes
  • Western blot - Anti-p53 antibody [PAb 240] (ab26)
    Western blot - Anti-p53 antibody [PAb 240] (ab26)
    Lanes 1-2 : Anti-p53 antibody [PAb 240] (ab26) at 1 µg/ml
    Lanes 3-4 : Anti-p53 antibody [PAb 240] (ab26) at 5 µg/ml

    All lanes : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 53 kDa


    Exposure time: 4 minutes


    Lanes 1-2: 1% BSA blocking buffer

    Lanes 3-4: 3% Milk blocking buffer

    We recommend using 3% milk as the blocking agent for Western blot.

  • Western blot - Anti-p53 antibody [PAb 240] (ab26)
    Western blot - Anti-p53 antibody [PAb 240] (ab26)This image is courtesy of an Abreview submitted by Dr Cherie Blenkiron
    All lanes : Anti-p53 antibody [PAb 240] (ab26) at 1/2000 dilution

    Lane 1 : Human breast cancer cell-line, MCF7 cells (p53 WT), whole cell lysate
    Lane 2 : Human breast cancer cell-line, MDA231 cells (p53 Mutant), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP conjugated donkey anti-mouse antibody at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 53 kDa
    Observed band size: 53 kDa
    Additional bands at: 72 kDa (possible non-specific binding)


    Exposure time: 10 seconds

    See Abreview

  • Western blot - Anti-p53 antibody [PAb 240] (ab26)
    Western blot - Anti-p53 antibody [PAb 240] (ab26)

    Primary: All Lanes: Anti-p53 antibody (ab26) at 5 µg/mL. Lane 1: MW marker. Lane 2: NIH/3T3 cells treated with vehicle for 24 hours. Lane 3: NIH/3T3 cells treated with 1 µM doxorubicin for 24 hours Secondary: All Lanes: HRP-conjugated VeriBlot anti-Mouse IgG (ab131368) 1:1000. Lysates at 20 µg/lane. Performed under denaturing conditions. Developed using ECL technique. Blocking buffer: 5% milk in PBS.

实验方案

  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (212)

发表研究结果有使用 ab26?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab26 被引用在 212 文献中.

  • Ma C  et al. The pro-inflammatory effect of NR4A3 in osteoarthritis. J Cell Mol Med 24:930-940 (2020). PubMed: 31701670
  • Li Q  et al. Silencing of synaptotagmin 13 inhibits tumor growth through suppressing proliferation and promoting apoptosis of colorectal cancer cells. Int J Mol Med 45:234-244 (2020). PubMed: 31939613
  • Gao L  et al. TNAP inhibition attenuates cardiac fibrosis induced by myocardial infarction through deactivating TGF-ß1/Smads and activating P53 signaling pathways. Cell Death Dis 11:44 (2020). PubMed: 31969558
  • Miao J  et al. Nuclear HMGB1 promotes the phagocytic ability of macrophages. Exp Cell Res 393:112037 (2020). PubMed: 32360192
  • Xu C  et al. Genetically multimodal therapy mediated by one polysaccharides-based supramolecular nanosystem. Biomaterials 248:120031 (2020). PubMed: 32283391
View all Publications for this product

客户评价及客户问答

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1-10 of 83 Abreviews or Q&A

Western blot abreview for Anti-p53 antibody [PAb 240]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
60 µg
Gel Running Conditions
Reduced Non-Denaturing (Native) (12)
Sample
Mouse Tissue lysate - whole (Mouse Testis)
Specification
Mouse Testis
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Read More

Dr. Francesco Elia Marino

Verified customer

提交于 May 15 2014

Immunocytochemistry/ Immunofluorescence abreview for Anti-p53 antibody [PAb 240]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Dog Cell (MDCK-t2)
Permeabilization
Yes - 0.5% Triton X-100 in PBS, 5min, RT
Specification
MDCK-t2
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

提交于 Mar 01 2021

Western blot abreview for Anti-p53 antibody [PAb 240]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (U87 cells)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-20)
Loading amount
13 µg
Treatment
bevacizumab 0, 1 and 2mg/ml for 48hrs
Specification
U87 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More

Mrs. Kyunga Kwak

Verified customer

提交于 May 08 2020

Western blot abreview for Anti-p53 antibody [PAb 240]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (ovarian and colon)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
30 µg
Treatment
3.2uM of drug for various time points
Specification
ovarian and colon
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

提交于 Aug 21 2019

Western blot abreview for Anti-p53 antibody [PAb 240]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (PNT2 prostate tissue)
Gel Running Conditions
Reduced Denaturing (7)
Loading amount
30 µg
Specification
PNT2 prostate tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Verified customer

提交于 Jan 08 2019

Western blot abreview for Anti-p53 antibody [PAb 240]

Below Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rabbit Tissue lysate - whole (Brain)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
0.2 µg
Treatment
hypoxic 40 minutes at E25
Specification
Brain
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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Verified customer

提交于 Jul 20 2018

Western blot abreview for Anti-p53 antibody [PAb 240]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse neural stem cell)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
40 µg
Specification
mouse neural stem cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Abcam user community

Verified customer

提交于 Jun 12 2018

Western blot abreview for Anti-p53 antibody [PAb 240]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Dog Cell lysate - whole cell (CPEK)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
CPEK
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5%
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Abcam user community

Verified customer

提交于 Apr 17 2015

Western blot abreview for Anti-p53 antibody [PAb 240]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (12)
Sample
Human Cell lysate - whole cell (LNCaP)
Specification
LNCaP
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
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Abcam user community

Verified customer

提交于 Jan 16 2015

Flow Cytometry abreview for Anti-p53 antibody [PAb 240]

Poor
Abreviews
Abreviews
abreview image
Application
Flow Cytometry
Fixation
Methanol
Permeabilization
Yes - 0.1% Tween
Sample
Human Cell (MDA-MB-231)
Specification
MDA-MB-231
Gating Strategy
Live cells gated
Preparation
Cell harvesting/tissue preparation method: Cell dissociation buffer
Sample buffer: Enzyme free
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Abcam user community

Verified customer

提交于 Dec 29 2014

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