Application
Western blot
Sample
Human Cell lysate - whole cell (HT1080+MCF7 cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
HT1080+MCF7 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 24°C · Diluent: 5% BSA in PBS+0.5% Tween 20
Dilution
1/1000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: anti mouse
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL plus
Exposure
30 second(s)
Bands
Specific: 58 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Oct 26 2011