Application
Western blot
Sample
Mouse Cell lysate - other (MEF)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
MEF
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: TBST
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: RABBIT ANTI-MOUSE
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
2 second(s)
Bands
Specific: 58 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 May 05 2011