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Tags & Cell Markers Subcellular Markers Organelles ER

Anti-P4HB抗体[RL90] (ab2792)

  • Datasheet
  • SDS
Reviews (48)Q&A (17)References (195)

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Western blot - Anti-P4HB antibody [RL90] (ab2792)
  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HB antibody [RL90] (ab2792)
  • Flow Cytometry - Anti-P4HB antibody [RL90] (ab2792)
  • Western blot - Anti-P4HB antibody [RL90] (ab2792)
  • Western blot - Anti-P4HB antibody [RL90] (ab2792)
  • Western blot - Anti-P4HB antibody [RL90] (ab2792)
  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HB antibody [RL90] (ab2792)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HB antibody [RL90] (ab2792)
  • Flow Cytometry - Anti-P4HB antibody [RL90] (ab2792)
  • Flow Cytometry - Anti-P4HB antibody [RL90] (ab2792)
  • Flow Cytometry - Anti-P4HB antibody [RL90] (ab2792)

Key features and details

  • Mouse monoclonal [RL90] to P4HB
  • Suitable for: ELISA, Inhibition Assay, IHC-Fr, IHC-P, WB, IP, Flow Cyt, ICC/IF, Electron Microscopy
  • Reacts with: Mouse, Rat, Hamster, Dog, Human, Pig, Monkey, African green monkey
  • Isotype: IgG2a

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概述

  • 产品名称

    Anti-P4HB抗体[RL90]
    参阅全部 P4HB 一抗
  • 描述

    小鼠单克隆抗体[RL90] to P4HB
  • 宿主

    Mouse
  • 经测试应用

    适用于: ELISA, Inhibition Assay, IHC-Fr, IHC-P, WB, IP, Flow Cyt, ICC/IF, Electron Microscopymore details
  • 种属反应性

    与反应: Mouse, Rat, Hamster, Dog, Human, Pig, Monkey, African green monkey
    预测可用于: Drosophila melanogaster
  • 免疫原

    Full length native protein (purified) corresponding to Rat P4HB. Purified rat PDIA1/P4HB protein.
    Database link: P04785

  • 阳性对照

    • WB: HeLa, A-431,NIH/3T3, A-375, Hep G2, HL-60, and PC-12.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, PBS
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    RL90
  • 同种型

    IgG2a
  • 研究领域

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • ER
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • Collagen
    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Other Chaperones
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • Assay kits

    • P4HB Inhibitor Screening Assay Kit (ab139480)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2792于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA
Use at an assay dependent concentration.
Inhibition Assay
Use at an assay dependent concentration.
IHC-Fr (1)
Use at an assay dependent concentration.
IHC-P (2)
1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
WB (15)
1/1000. Detects a band of approximately 59-61 kDa (predicted molecular weight: 58 kDa). If there is no signal or signal is weak, more concentrated antibody could be used in addition to using less stringent blocking conditions (e.g., BSA instead of milk, incubating the antibody in PBST or TBST only, lower milk percentage).
IP
Use at an assay dependent concentration.

This antibody has been shown to inhibit the activity of PDI in vitro. It has also been found to inhibit disulfide bond reduction of the HIV protein, gp120, at the cell surface of CHO cells and human lymphoid cells.

Flow Cyt
Use 0.5µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ICC/IF (26)
Use at an assay dependent concentration.
Electron Microscopy
Use at an assay dependent concentration. PubMed: 21886772
说明
ELISA
Use at an assay dependent concentration.
Inhibition Assay
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
IHC-P
1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 59-61 kDa (predicted molecular weight: 58 kDa). If there is no signal or signal is weak, more concentrated antibody could be used in addition to using less stringent blocking conditions (e.g., BSA instead of milk, incubating the antibody in PBST or TBST only, lower milk percentage).
IP
Use at an assay dependent concentration.

This antibody has been shown to inhibit the activity of PDI in vitro. It has also been found to inhibit disulfide bond reduction of the HIV protein, gp120, at the cell surface of CHO cells and human lymphoid cells.

Flow Cyt
Use 0.5µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ICC/IF
Use at an assay dependent concentration.
Electron Microscopy
Use at an assay dependent concentration. PubMed: 21886772

靶标

  • 功能

    This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP.
  • 序列相似性

    Belongs to the protein disulfide isomerase family.
    Contains 2 thioredoxin domains.
  • 细胞定位

    Endoplasmic reticulum lumen. Melanosome. Cell membrane. Highly abundant. In some cell types, seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources (Probable). Localizes near CD4-enriched regions on lymphoid cell surfaces. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession P07237 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 483369 Dog
    • Entrez Gene: 39651 Drosophila melanogaster
    • Entrez Gene: 5034 Human
    • Entrez Gene: 18453 Mouse
    • Entrez Gene: 110255932 Pig
    • Entrez Gene: 25506 Rat
    • Omim: 176790 Human
    • SwissProt: P54399 Drosophila melanogaster
    • SwissProt: P07237 Human
    • SwissProt: P09103 Mouse
    • SwissProt: P04785 Rat
    • Unigene: 2710 Drosophila melanogaster
    • Unigene: 464336 Human
    • Unigene: 708779 Human
    • Unigene: 16660 Mouse
    • Unigene: 392196 Mouse
    • Unigene: 4234 Rat
    see all
  • 别名

    • Cellular thyroid hormone binding protein antibody
    • Cellular thyroid hormone-binding protein antibody
    • Collagen prolyl 4 hydroxylase beta antibody
    • Disulphide Isomerase antibody
    • DSI antibody
    • EC 5.3.4.1 antibody
    • Endoplasmic reticulum resident protein 59 antibody
    • ER protein 59 antibody
    • ERBA2L antibody
    • ERp59 antibody
    • GIT antibody
    • Gltathione insulin transhydrogenase antibody
    • Glutathione insulin transhydrogenase antibody
    • P4HB antibody
    • P4Hbeta antibody
    • p55 antibody
    • PDI antibody
    • PDIA1 antibody
    • PDIA1_HUMAN antibody
    • PDIR antibody
    • PHDB antibody
    • PO4DB antibody
    • PO4HB antibody
    • Procollagen proline 2 oxoglutarate 4 dioxygenase (proline 4 hydroxylase) beta polypeptide (protein disulfide isomerase associated 1) antibody
    • Procollagen proline 2 oxoglutarate 4 dioxygenase beta subunit antibody
    • PROHB antibody
    • Prolyl 4 hydroxylase beta polypeptide antibody
    • Prolyl 4 hydroxylase beta subunit antibody
    • Prolyl 4 hydroxylase subunit beta antibody
    • Prolyl 4-hydroxylase subunit beta antibody
    • Protein disulfide isomerase associated 1 antibody
    • Protein disulfide isomerase, family A, member 1 antibody
    • Protein disulfide isomerase/oxidoreductase antibody
    • Protein disulfide-isomerase antibody
    • Protocollagen hydroxylase antibody
    • Thbp antibody
    • Thyroid hormone binding protein p55 antibody
    • Thyroid hormone binding protein p55 cellular antibody
    • V erb a avian erythroblastic leukemia viral oncogene homolog 2 like antibody
    see all

图片

  • Western blot - Anti-P4HB antibody [RL90] (ab2792)
    Western blot - Anti-P4HB antibody [RL90] (ab2792)
    All lanes : Anti-P4HB antibody [RL90] (ab2792) at 1/2000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
    Lane 4 : A-375 (human malignant melanoma cell line) whole cell lysate
    Lane 5 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 6 : HL-60 (human promyelocytic leukemia cell line) whole cell lysate
    Lane 7 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat anti-Mouse IgG H+L (HRP) at 1/4000 dilution

    Predicted band size: 58 kDa
    Observed band size: 57 kDa why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
    Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)

    Immunocytochemistry/Immunofluorescence analysis of P4HB (PDIA1) (red) in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with ab2792 (1:75) for at least 1 hour at room temperature and incubated with DyLight 550 goat anti-mouse IgG secondary antibody (1:250) for 30 minutes at room temperature. Actin was stained with DyLight 488 Phalloidin at a dilution of 1:300 (1 unit/ml final concentration) for 30 minutes. Images were taken at 20X magnification.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HB antibody [RL90] (ab2792)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HB antibody [RL90] (ab2792)

    Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human colon tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing P4HB (PDIA1) ab2792 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Flow Cytometry - Anti-P4HB antibody [RL90] (ab2792)
    Flow Cytometry - Anti-P4HB antibody [RL90] (ab2792)

    Overlay histogram showing HeLa cells stained with ab2792 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2792, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Western blot - Anti-P4HB antibody [RL90] (ab2792)
    Western blot - Anti-P4HB antibody [RL90] (ab2792)

    Western blot analysis of P4HB (PDIA1) was performed by loading 25 ug of HepG2 (Lane 1) Hela (Lane 2) and NIH-3T3 (Lane 3) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with ab2792 at 1:1000 overnight at 4°C and washed in TBST. The membrane was then probed with a HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using a ECL Plus Western Blotting Substrate. Results show a band at approx. 57 kDa.

  • Western blot - Anti-P4HB antibody [RL90] (ab2792)
    Western blot - Anti-P4HB antibody [RL90] (ab2792)This image is courtesy of an anonymous Abreview
    Anti-P4HB antibody [RL90] (ab2792) at 1/2000 dilution
    Secondary
    Donkey anti mouse IgG2a at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 58 kDa
    Observed band size: 57 kDa why is the actual band size different from the predicted?


    Exposure time: 20 seconds

    See Abreview

  • Western blot - Anti-P4HB antibody [RL90] (ab2792)
    Western blot - Anti-P4HB antibody [RL90] (ab2792)
    Anti-P4HB antibody [RL90] (ab2792) at 1/1000 dilution + HT1080 whole cell lysate at 20000 cells

    Secondary
    HRP-conjugated sheep anti-mouse IgG at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 58 kDa
    Observed band size: 57 kDa why is the actual band size different from the predicted?


    Exposure time: 20 seconds


    10% SDS-PAGE.

    Blocked with 5% milk for 1 hour at 22°C.

    Incubated with the primary for 16 hours at 4°C in PBS + 2.5% milk + 0.05% Tween20.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
    Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)This image is courtesy of an anonymous Abreview

    ab2792 staining P4HB (PDIA1) in MDA MB 231 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 1% Triton X-100 and blocked with 10% BSA for 1 hour at 21°C. Samples were incubated with primary antibody (1/100 in BSA + 0.02% Tween 20) for 1 hour at 16°C. A DyLight® 550-conjugated goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
    Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)

    Immunocytochemistry/Immunofluorescence analysis of P4HB (PDIA1) (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with ab2792 (1:75) for at least 1 hour at room temperature and incubated with Dylight 488 goat anti-mouse IgG secondary antibody (1:250) for 30 minutes at room temperature. Actin was stained with Dylight 350 Phalloidin at a dilution of 1:120 (2.5units/ml final concentration) and nuclei (red) were stained with DRAQ5 at a concentration of 1ug/ml for 30 minutes. Images were taken at 20X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
    Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)

    Immunocytochemistry/Immunofluorescence analysis of P4HB (PDIA1) (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were incubated with ab2792 (1:75) for at least 1 hour at room temperature and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (1:250) for 30 minutes at room temperature. Actin was stained with DyLight 550 Phalloidin at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst at a concentration of 1ug/ml for 30 minutes. Images were taken at 20X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
    Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)

    Immunocytochemistry/Immunofluorescence analysis of P4HB (PDIA1) (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were incubated with ab2792 (1:75) for at least 1 hour at room temperature and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (1:250) for 30 minutes at room temperature. Actin was stained with DyLight 650 Phalloidin at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst at a concentration of 1ug/ml for 30 minutes. Images were taken at 20X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
    Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)

    Immunocytochemistry/Immunofluorescent analysis of P4HB (PDIA1) using ab2792 shows staining in p19 Cells.

  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
    Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)

    Immunocytochemistry/Immunofluorescent analysis of P4HB (PDIA1) using ab2792 shows staining in NS-1 Cells.

  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
    Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)

    Immunocytochemistry/Immunofluorescent analysis of P4HB (PDIA1) using ab2792 shows staining in HMVEC Cells.

  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
    Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)

    Immunocytochemistry/Immunofluorescent analysis of P4HB (PDIA1) using ab2792 shows staining in A549 Cells.

  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
    Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)

    ab2792 positively staining dog MDCK cell ER (red) at 1/200. Staining was carried out in conjunction with goat anti mouse H and L ( Alexa 546) at 1/1000. The nuclei can be seen stained with Hoechst (blue)
    This image is courtesy of an Abreview submitted by Kun Liu on 20 September 2005. We do not have any further information relating to this image.

  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
    Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)

    ab2792 positively staining formaldehyde fixed human Hek293 cells (1/200). This Ab was used in conjunction with goat anti mouse Alexa Fluor® 546 (1/1500). The nuclei has been atained with Hoechst.
    This image is courtesy of an Abreview submitted by Kun Liu on 19th September 2005. We do not have any further information relating to this image.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
    Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)This image is a courtesy of Anonymous Abreview

    ab2792 staining P4HB (PDIA1) from human HaCaT keratinocyte cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with 0.25 % Triton ×100 and blocking with 2.5% BSA plus 1% goat serum for 1 hour at 4°C was performed. Samples were incubated with primary antibody, diluted 1/100, for 1 hour at 240C. An Alexa Fluor® 488-conjugated goat polyclonal to mouse IgG was used at dilution at 1/1000 as secondary antibody.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HB antibody [RL90] (ab2792)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HB antibody [RL90] (ab2792)

    Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human tonsil tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing P4HB (PDIA1) ab2792 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HB antibody [RL90] (ab2792)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HB antibody [RL90] (ab2792)

    Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human lung adenocarcinoma tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing P4HB (PDIA1) ab2792 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Flow Cytometry - Anti-P4HB antibody [RL90] (ab2792)
    Flow Cytometry - Anti-P4HB antibody [RL90] (ab2792)

    Flow cytometry analysis of P4HB (PDIA1) showing positive staining in the membrane and cytoplasm of K562 cells compared to an isotype control (blue). Cells were harvested and adjusted to a concentration of 1-5x10^6 cells/ml. Cells were then fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with ab2792 at 1 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

  • Flow Cytometry - Anti-P4HB antibody [RL90] (ab2792)
    Flow Cytometry - Anti-P4HB antibody [RL90] (ab2792)

    Flow cytometry analysis of P4HB (PDIA1) showing positive staining in the membrane and cytoplasm of NIH/3T3 cells compared to an isotype control (blue). Cells were harvested and adjusted to a concentration of 1-5x10^6 cells/ml. Cells were then fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with ab2792 at 0.5 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

  • Flow Cytometry - Anti-P4HB antibody [RL90] (ab2792)
    Flow Cytometry - Anti-P4HB antibody [RL90] (ab2792)

    Flow cytometry analysis of P4HB (PDIA1) showing positive staining in the membrane and cytoplasm of Hela cells compared to an isotype control (blue). Cells were harvested and adjusted to a concentration of 1-5x10^6 cells/ml. Cells were then fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with ab2792 at 1 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

实验方案

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

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文献 (195)

发表研究结果有使用 ab2792?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab2792 被引用在 195 文献中.

  • Lee DS & Kim JE Protein disulfide isomerase-mediated S-nitrosylation facilitates surface expression of P2X7 receptor following status epilepticus. J Neuroinflammation 18:14 (2021). PubMed: 33407649
  • Jiang S  et al. Hydrogen sulphide reduces hyperhomocysteinaemia-induced endothelial ER stress by sulfhydrating protein disulphide isomerase to attenuate atherosclerosis. J Cell Mol Med 25:3437-3448 (2021). PubMed: 33675119
  • Petkovic M  et al. TMEM16K is an interorganelle regulator of endosomal sorting. Nat Commun 11:3298 (2020). PubMed: 32620747
  • Tischer A  et al. Evidence for the Misfolding of the A1 Domain within Multimeric von Willebrand Factor in Type 2 von Willebrand Disease. J Mol Biol 432:305-323 (2020). PubMed: 31628947
  • Jia J  et al. AMPK, a Regulator of Metabolism and Autophagy, Is Activated by Lysosomal Damage via a Novel Galectin-Directed Ubiquitin Signal Transduction System. Mol Cell 77:951-969.e9 (2020). PubMed: 31995728
View all Publications for this product

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1-10 of 65 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-P4HB antibody [RL90]

Poor
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6
Permeabilization
No
Specification
skin
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

提交于 Feb 08 2021

Western blot abreview for Anti-P4HB antibody [RL90]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - other (Macrophages)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
10 µg
Specification
Macrophages
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

提交于 Nov 08 2018

Western blot abreview for Anti-P4HB antibody [RL90]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (heart)
Gel Running Conditions
Non-reduced Denaturing (10%)
Loading amount
20 µg
Specification
heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Abcam user community

Verified customer

提交于 Sep 07 2018

Immunocytochemistry/ Immunofluorescence abreview for Anti-P4HB antibody [RL90]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (heart)
Permeabilization
Yes - 0.2% Triton-X
Specification
heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

提交于 Sep 03 2018

Immunocytochemistry/ Immunofluorescence abreview for Anti-P4HB antibody [RL90]

Inconclusive
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Hamster Cell (Chinese Hamster Ovary)
Permeabilization
Yes - 0,1 % TritonX-100 for 5 min @ RT
Specification
Chinese Hamster Ovary
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Fixative
Formaldehyde
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Abcam user community

Verified customer

提交于 Dec 20 2016

IHC - Wholemount abreview for Anti-P4HB antibody [RL90]

Inconclusive
Abreviews
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Drosophila melanogaster Tissue (pupae wings)
Specification
pupae wings
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Abcam user community

Verified customer

提交于 Apr 23 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-P4HB antibody [RL90]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (MDA MB 231 cells)
Permeabilization
Yes - 1% Triton
Specification
MDA MB 231 cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Fixative
Formaldehyde
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Abcam user community

Verified customer

提交于 Feb 19 2015

Western blot abreview for Anti-P4HB antibody [RL90]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH 3T3)
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE)
Loading amount
50 µg
Specification
NIH 3T3
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

提交于 Jul 25 2014

Immunocytochemistry/ Immunofluorescence abreview for Anti-P4HB antibody [RL90]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
African green monkey Cell (COS-7)
Permeabilization
Yes - Saponin 0.05%
Specification
COS-7
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

提交于 Jan 31 2014

Immunocytochemistry/ Immunofluorescence abreview for Anti-P4HB antibody [RL90]

Poor
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Xenopus laevis Cell (embryonic ectoderm)
Permeabilization
Yes - tween
Specification
embryonic ectoderm
Blocking step
BSA+milk+serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More

Dr. Sally Moody

Verified customer

提交于 May 28 2013

1-10 of 65 Abreviews or Q&A

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