产品名称Anti-p38 (phospho T180)抗体[EPR18120] - BSA and Azide free
参阅全部 p38 一抗
描述兔单克隆抗体[EPR18120] to p38 (phospho T180) - BSA and Azide free
经测试应用适用于: WB, Dot blotmore details
种属反应性与反应: Mouse, Human
Synthetic peptide within Human p38 aa 150-250 (phospho T180 + Y182). The exact sequence is proprietary.
Database link: Q16539
- WB: HEK-293 whole cell lysates treated with UV (60 J/M2) for 60 minutes.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Concentration information loading...
纯度Protein A purified
Our Abpromise guarantee covers the use of ab240335 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).|
|Dot blot||Use at an assay dependent concentration.|
功能Responds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.
组织特异性Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.
序列相似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.
结构域The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
翻译后修饰Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme.
Phosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- CSAID Binding Protein 1 antibody
- CSAID binding protein antibody
- CSAID-binding protein antibody
All lanes : Anti-p38 (phospho T180) antibody [EPR18120] - BSA and Azide free (ab240335) at 1/1000 dilution
Lane 1 : Untreated HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysates
Lane 2 : HEK-293 whole cell lysates treated with UV (60 J/M2) for 60 minutes
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
Predicted band size: 41 kDa
Blocking/Dilution buffer: 5% NFDM/TBST
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab195049).
ab240335 has not yet been referenced specifically in any publications.