重组Anti-p38 (phospho T180 + Y182)抗体[EPR18120] (ab195049)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18120] to p38 (phospho T180 + Y182)
- Suitable for: WB, Dot blot
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
-
产品名称
Anti-p38 (phospho T180 + Y182)抗体[EPR18120]
参阅全部 p38 一抗 -
描述
兔单克隆抗体[EPR18120] to p38 (phospho T180 + Y182) -
宿主
Rabbit -
经测试应用
适用于: WB, Dot blotmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- WB: HEK-293 whole cell lysate treated with UV (60 J/M2) for 60 minutes; NIH/3T3 whole cell lysate treated with UV (60 J/M2) for 60 minutes.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR18120 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab195049于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
|
|
Dot blot |
1/1000.
|
说明 |
---|
WB
1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa). |
Dot blot
1/1000. |
靶标
-
功能
Responds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis. -
组织特异性
Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney. -
序列相似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
结构域
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
翻译后修饰
Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 1432 Human
- Entrez Gene: 26416 Mouse
- Omim: 600289 Human
- SwissProt: Q16539 Human
- SwissProt: P47811 Mouse
- Unigene: 485233 Human
- Unigene: 311337 Mouse
-
别名
- CSAID Binding Protein 1 antibody
- CSAID binding protein antibody
- CSAID-binding protein antibody
see all
图片
-
Dot blot analysis of p38 (phospho T180) peptide (Lane 1), p38 (phospho Y182) (Lane 2), p38 (phospho T180 + Y182) peptide (Lane 3), and non-phospho peptide (Lane 4), labeled using ab195049 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody (ab97051) at 1/1000 dilution.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
-
All lanes : Anti-p38 (phospho T180 + Y182) antibody [EPR18120] (ab195049) at 1/1000 dilution
Lane 1 : Untreated HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysates
Lane 2 : HEK-293 whole cell lysates treated with UV (60 J/M2) for 60 minutes
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
-
All lanes : Anti-p38 (phospho T180 + Y182) antibody [EPR18120] (ab195049) at 1/1000 dilution
Lane 1 : Untreated NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
Lane 2 : NIH/3T3 whole cell lysate treated with UV (60 J/M2) for 60 minutes
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
数据表及文件
-
SDS download
-
Datasheet download
Certificate of Compliance
文献 (61)
ab195049 被引用在 61 文献中.
- Zhao C et al. Vitamin K3 Suppresses Pyroptosis in THP-1 Cells through Inhibition of NF-κB and JNK Signaling Pathways. Biol Pharm Bull 46:52-60 (2023). PubMed: 36288961
- Hsu YC et al. Administration of N-Acetylcysteine to Regress the Fibrogenic and Proinflammatory Effects of Oxidative Stress in Hypertrophic Ligamentum Flavum Cells. Oxid Med Cell Longev 2022:1380353 (2022). PubMed: 36338342
- Gao ZK et al. Pre-ischemic exercise prevents inflammation and apoptosis by inhibiting MAPK pathway in ischemic stroke. Transl Neurosci 13:495-505 (2022). PubMed: 36636513
- Huang Z et al. MiR-146a alleviates lung injury caused by RSV infection in young rats by targeting TRAF-6 and regulating JNK/ERKMAPK signaling pathways. Sci Rep 12:3481 (2022). PubMed: 35241728
- Gao X et al. Piceatannol suppresses inflammation and promotes apoptosis in rheumatoid arthritis‑fibroblast‑like synoviocytes by inhibiting the NF‑κB and MAPK signaling pathways. Mol Med Rep 25:N/A (2022). PubMed: 35322865