Application
Western blot
Sample
Mouse Tissue lysate - whole (skeletal muscle)
Loading amount
20 µg
Specification
skeletal muscle
Gel Running Conditions
Reduced Denaturing (Bis-Tris 4-12% MOPS running buffer)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
Pierce's Dura
Exposure
30 minute(s) and 0 second(s)
Bands
Specific: ~39 kDa Non-specific: 28, 45 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Feb 25 2008