Anti-p38 (phospho T180 + Y182)抗体(ab4822)
Key features and details
- Rabbit polyclonal to p38 (phospho T180 + Y182)
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-p38 (phospho T180 + Y182)抗体
参阅全部 p38 一抗 -
描述
兔多克隆抗体to p38 (phospho T180 + Y182) -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IF, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Dog, Carp, Monkey
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免疫原
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阳性对照
- HEK 293 cells +/- UV irradiation treatment and PC12 cells +/- Sorbitol.
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常规说明
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA
BSA is IgG and protease free. PBS without Mg2+ and Ca2+. -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using i) non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated p38, and ii) a JNK-derived peptide that is phosphorylated at threonine 183 and tyrosine 185. The final product is generated by affinity chromatography using a p38-derived peptide that is phosphorylated at threonine 180 and tyrosine 182. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
Our Abpromise guarantee covers the use of ab4822 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/10 - 1/100. | |
| ICC/IF | 1/250. | |
| WB | 1/1000. Predicted molecular weight: 38 kDa. |
靶标
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功能
Responds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis. -
组织特异性
Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney. -
序列相似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
结构域
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
翻译后修饰
Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 403856 Dog
- Entrez Gene: 1432 Human
- Entrez Gene: 26416 Mouse
- Omim: 600289 Human
- SwissProt: O02812 Dog
- SwissProt: Q16539 Human
- SwissProt: P47811 Mouse
- Unigene: 485233 Human
see all -
别名
- CSAID Binding Protein 1 antibody
- CSAID binding protein antibody
- CSAID-binding protein antibody
see all
图片
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Western blot - Anti-p38 (phospho T180 + Y182) antibody (ab4822)All lanes : Anti-p38 (phospho T180 + Y182) antibody (ab4822) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) exposed for 40 min with UV, cell lysate
Lane 3 : A431 (human epidermoid carcinoma cell line) cell lysate
Lane 4 : A431 (human epidermoid carcinoma cell line) exposed for 40 min with UV, cell lysate
Lane 5 : COLO 205 (human colon adenocarcinoma cell line) cell lysate
Lane 6 : COLO 205 (human colon adenocarcinoma cell line) exposed for 40 min with UV, cell lysate
Lane 7 : A549 (human lung carcinoma cell line) cell lysate
Lane 8 : A549 (human lung carcinoma cell line) exposed for 40 min with UV, cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG HRP at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 38 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38 (phospho T180 + Y182) antibody (ab4822)Paraffin-embedded human brain tissue stained for p38 (phospho T180 + Y182) using ab4822 (right panel) at 1/100 dilution in immunohistochemical analysis followed by HRP-conjugated secondary antibody and DAB staining. Negative control (left panel) staining without primary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38 (phospho T180 + Y182) antibody (ab4822)Paraffin-embedded human heart tissue stained for p38 (phospho T180 + Y182) using ab4822 (right panel) at 1/20 dilution in immunohistochemical analysis followed by HRP-conjugated secondary antibody and DAB staining. Negative control (left panel) staining without primary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38 (phospho T180 + Y182) antibody (ab4822)Paraffin-embedded rat heart tissue stained for p38 (phospho T180 + Y182) using ab4822 (right panel) at 1/20 dilution in immunohistochemical analysis followed by HRP-conjugated secondary antibody and DAB staining. Negative control (left panel) staining without primary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-p38 (phospho T180 + Y182) antibody (ab4822)4% PFA-fixed, Triton X-100 permeabilized SH-SY5Y (human neuroblastoma cell line from bone marrow) cells labeling p38 (phospho T180 + Y182) (Panel A: green) using ab4822 at 1 µg/mL in ICC/IF. Secondary antibody: Alexa Flour® 488 Goat Anti-Rabbit IgG at 1/400 dilution. Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 594 Phalloidin. Panel d is a merged image showing nuclear localization. Panel e is a no primary antibody control.
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Western blot - Anti-p38 (phospho T180 + Y182) antibody (ab4822)Peptide Competition: Extracts prepared from HEK-293 (human epithelial cell line from embryonic kidney) cells treated with UV irradiation were resolved on a 10% Tris-glycine gel and transferred to nitrocellulose. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with 0.50 µg/mL ab4822 for two hours at room temperature in a 3% BSA-TBST buffer, following its prior incubation with: the peptide immunogen (1), a generic phosphothreonine containing peptide (2), a generic phosphotyrosine-containing peptide (3), the non-phosphorylated peptide corresponding to the phosphopeptide (4), no peptide (5), the phosphorylated peptide derived from the corresponding region of JNK 1 & 2 (6), and, the phosphorylated peptide derived from the corresponding region of ERK 1 & 2 (7). After washing, membranes were incubated with goat F(ab')2 antirabbit IgG alkaline phosphatase and the signal was detected using the Tropix WesternStar method. The data show that only the phosphopeptide
实验方案
文献 (100)
ab4822 被引用在 100 文献中.
- Xiang Y et al. LncRNA MEG3 targeting miR-424-5p via MAPK signaling pathway mediates neuronal apoptosis in ischemic stroke. Aging (Albany NY) 12:3156-3174 (2020). PubMed: 32065781
- Hu DD et al. SKA3 promotes lung adenocarcinoma metastasis through the EGFR-PI3K-Akt axis. Biosci Rep 40:N/A (2020). PubMed: 32068236
- Liang X et al. Bushen Tiaojing (II and III) Decoctions Activate MAPK14 and MAPK3/1 to Promote the Expression of Cumulus Expansion-Related Factors in Mice. Evid Based Complement Alternat Med 2020:9283917 (2020). PubMed: 32148554
- Li X et al. MCM3AP-AS1 KD Inhibits Proliferation, Invasion, and Migration of PCa Cells via DNMT1/DNMT3 (A/B) Methylation-Mediated Upregulation of NPY1R. Mol Ther Nucleic Acids 20:265-278 (2020). PubMed: 32193153
- Sun C et al. ADAM17-regulated CX3CL1 expression produced by bone marrow endothelial cells promotes spinal metastasis from hepatocellular carcinoma. Int J Oncol 57:249-263 (2020). PubMed: 32319605
