Western blot abreview for Anti-p38 antibody [E229]

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Treatment
UV (100 J/m2)
Specification
HeLa
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 5% BSA
Dilution
1/2000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: Anti-Rabbit IgG, HRP-Linked Whole Ab Donkey
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
10 second(s)
Bands
Specific: 38 kDa

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提交于 Nov 12 2018

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