Western blot abreview for Anti-p38 antibody [E229]

Good
Abreviews
Application
Western blot
Sample
Zebrafish Tissue lysate - whole (whole fish 7-14 days post fertilization)
Gel Running Conditions
Reduced Denaturing (12% BioRad)
Loading amount
20 µg
Specification
whole fish 7-14 days post fertilization
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBST
Dilution
1/200

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: BioRad goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Negative control
none
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 38 kDa
Positive control
HEK lysate 30ug

Additional data

Additional Notes
Lanes 1-3 whole zebrafish lysate 7 days post fertilization Lanes 4-6 whole zebrafish lysate 10 days post fertilization Lanes 7-9 whole zebrafish lysate 14 days post fertilization

Jennifer Rahn

Verified customer

提交于 Mar 07 2016

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