Application
Western blot
Sample
Zebrafish Tissue lysate - whole (whole fish 7-14 days post fertilization)
Gel Running Conditions
Reduced Denaturing (12% BioRad)
Loading amount
20 µg
Specification
whole fish 7-14 days post fertilization
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBST
Dilution
1/200
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: BioRad goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
none
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 38 kDa
Positive control
HEK lysate 30ug
Additional data
Additional Notes
Lanes 1-3 whole zebrafish lysate 7 days post fertilization
Lanes 4-6 whole zebrafish lysate 10 days post fertilization
Lanes 7-9 whole zebrafish lysate 14 days post fertilization
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Jennifer Rahn
Verified customer
提交于 Mar 07 2016