Application
ChIP
Sample
Mouse Cell lysate - nuclear (MEFs)
Negative control
MEFs KO for p38
Specification
MEFs
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehide 1%
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehide 1%
Positive control
MEFs treated with NaCl for 45 min to recrute p38 to Egr-1 Chromatin
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/100
Additional data
Additional Notes
ABTRIAL-TV7NH
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 May 15 2015