Anti-p21抗体[CIP1/823] - BSA and Azide free (ab212247)
Key features and details
- Mouse monoclonal [CIP1/823] to p21 - BSA and Azide free
- Suitable for: WB, IHC-P, ICC
- Knockout validated
- Reacts with: Human
- Isotype: IgG2a
概述
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产品名称
Anti-p21抗体[CIP1/823] - BSA and Azide free
参阅全部 p21 一抗 -
描述
小鼠单克隆抗体[CIP1/823] to p21 - BSA and Azide free -
宿主
Mouse -
经测试应用
适用于: WB, IHC-P, ICCmore details -
种属反应性
与反应: Human
不与反应: Mouse, Rat -
免疫原
Recombinant full length protein corresponding to Human p21 aa 1 to the C-terminus. NCBI Accession No. 370771.
Database link: P38936 -
阳性对照
- ICC: HeLa cells; WB: HeLa whole cell lysate; IHC-P: Skin, colon, breast carcinoma, lung squamous cell carcinoma, and bladder carcinoma tissues.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Constituent: 100% PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A/G purified -
克隆
单克隆 -
克隆编号
CIP1/823 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab212247于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 18 kDa).
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IHC-P |
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC |
Use a concentration of 1 - 2 µg/ml.
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说明 |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 18 kDa). |
IHC-P
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC
Use a concentration of 1 - 2 µg/ml. |
靶标
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功能
May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. -
组织特异性
Expressed in all adult human tissues, with 5-fold lower levels observed in the brain. -
序列相似性
Belongs to the CDI family. -
结构域
The PIP-box K+4 motif mediates both the interaction with PCNA and the recuitment of the DCX(DTL) complex: while the PIP-box interacts with PCNA, the presence of the K+4 submotif, recruits the DCX(DTL) complex, leading to its ubiquitination.
The C-terminal is required for nuclear localization of the cyclin D-CDK4 complex. -
翻译后修饰
Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA. Phosphorylation at Ser-114 by GSK3-beta enhances ubiquitination by the DCX(DTL) complex.
Ubiquitinated by MKRN1; leading to polyubiquitination and 26S proteasome-dependent degradation. Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, leading to its degradation during S phase or following UV irradiation. Ubiquitination by the DCX(DTL) complex is essential to control replication licensing and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 1026 Human
- Omim: 116899 Human
- SwissProt: P38936 Human
- Unigene: 370771 Human
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别名
- CAP20 antibody
- CDK-interacting protein 1 antibody
- CDKI antibody
see all
图片
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All lanes : Anti-p21 antibody [CIP1/823] (ab220206) at 1 µg/ml
Lane 1 : Wild-type HCT 116 cell lysate
Lane 2 : CDKN1A knockout HCT 116 cell lysate
Lane 3 : Wild-type MCF7 cell lysate
Lane 4 : CDKN1A knockout MCF7 cell lysate
Lane 5 : Wild-type A549 cell lysate
Lane 6 : CDKN1A knockout A549 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220206).
Western blot: Anti-CDKN1A antibody [CIP1/823] (ab220206) staining at 1 ug/ml, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab220206 was shown to bind specifically to CDKN1A. A band was observed at 21 kDa in wild-type cell lysates with no signal observed at this size in CDKN1A knockout cell lines. To generate this image, wild-type and CDKN1A knockout cell lysates were analysed. CDKN1A knockout cell lines in HCT 116 (ab288187), MCF7 (ab288200) and A549 (ab288213) backgrounds were used. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution.
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Anti-p21 antibody [CIP1/823] - BSA and Azide free (ab212247) at 2 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Predicted band size: 18 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted? -
Immunocytochemistry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling p21 with ab212247 followed by Goat anti-Mouse IgG-CF555 (Red). Membrane stained with Phalloidin 488 (Green).
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Immunohistochemical analysis of formalix-fixed paraffin-embedded Human lung squamous cell carcinoma tissue, labeling p21 using ab212247 at 4 µg/mL.
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Immunohistochemical analysis of formalix-fixed paraffin-embedded Human bladder carcinoma tissue, labeling p21 using ab212247 at 4 µg/mL.
数据表及文件
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Datasheet download
文献 (1)
ab212247 被引用在 1 文献中.
- Wei D et al. MicroRNA-30a-3p inhibits the progression of lung cancer via the PI3K/AKT by targeting DNA methyltransferase 3a. Onco Targets Ther 12:7015-7024 (2019). PubMed: 31695416