重组Anti-P Glycoprotein抗体[EPR10364] (ab168337)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10364] to P Glycoprotein
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-P Glycoprotein抗体[EPR10364]
参阅全部 P Glycoprotein 一抗 -
描述
兔单克隆抗体[EPR10364] to P Glycoprotein -
宿主
Rabbit -
特异性
P-glycoprotein 1 (also known as Multidrug resistance protein 1) has a predicted molecular weight of 141 kDa, however it has 3 potential glycosylation sites (N-linked) which may affect the migration of the protein. In our hands ab168337 detects a predominant protein band migrating in the region of 180-200 kDa and typically will demonstrate a smear on the membrane in the region of the 150 – 300 kDa due to the glycosylation profile of the protein. It may be necessary to optimise your cell or tissue lysis protocol to efficiently extract P-glycoprotein 1 as it is a multi-pass membrane protein. Abcam recommends not boiling the sample after lysis. -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment corresponding to Human P Glycoprotein.
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阳性对照
- HeLa, HepG2, 293T, C6 and Human fetal brain and Mouse brain lysates, Human kidney & liver tissue
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常规说明
The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR10364 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab168337于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/2000. Predicted molecular weight: 141 kDa.
For optimal detection Abcam recommends not boiling the sample after lysis. |
IHC-P |
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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WB
1/2000. Predicted molecular weight: 141 kDa. For optimal detection Abcam recommends not boiling the sample after lysis. |
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells. -
组织特异性
Expressed in liver, kidney, small intestine and brain. -
疾病相关
Genetic variations in ABCB1 are associated with susceptibility to inflammatory bowel disease type 13 (IBD13) [MIM:612244]. Inflammatory bowel disease is characterized by a chronic relapsing intestinal inflammation. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Crohn disease and ulcerative colitis are commonly classified as autoimmune diseases. -
序列相似性
Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 5243 Human
- Entrez Gene: 18669 Mouse
- Entrez Gene: 24646 Rat
- Omim: 171050 Human
- SwissProt: P08183 Human
- SwissProt: P06795 Mouse
- SwissProt: P43245 Rat
- Unigene: 489033 Human
see all -
别名
- ABC20 antibody
- ABCB1 antibody
- ATP binding cassette, sub family B (MDR/TAP), member 1 antibody
see all
图片
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Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: P glycoprotein knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: HepG2 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab168337 observed at 160 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab168337 was shown to recognize P glycoprotein when P glycoprotein knockout samples were used, along with additional cross-reactive bands. Wild-type and P glycoprotein knockout samples were subjected to SDS-PAGE. ab168337 and ab8245 (loading control to GAPDH) were diluted 1/500 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling P Glycoprotein with purified ab168337 at 1/100 dilution (14 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Hematoxylin was used to counter stain. ab97051, a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.
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All lanes : Anti-P Glycoprotein antibody [EPR10364] (ab168337) at 1/2000 dilution (purified)
Lane 1 : Mouse brain lysate
Lane 2 : C6 (Rat glial tumor cell line) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 141 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST.
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All lanes : Anti-P Glycoprotein antibody [EPR10364] (ab168337) at 1/2000 dilution (purified)
Lane 1 : HeLa (human cervix adenocarcinoma) whole cell lysate
Lane 2 : HepG2 (human hepatocellular carcinoma) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 141 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST.
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All lanes : Anti-P Glycoprotein antibody [EPR10364] (ab168337) at 1/1000 dilution (unpurified)
Lane 1 : HeLa cell lysates
Lane 2 : 293T cell lysates
Lane 3 : Human fetal brain lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 141 kDa -
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling P Glycoprotein with unpurified ab168337 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling P Glycoprotein with unpurified ab168337 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab168337 被引用在 8 文献中.
- Qiu R et al. Ginsenosides Rg1 and CK Control Temozolomide Resistance in Glioblastoma Cells by Modulating Cholesterol Efflux and Lipid Raft Distribution. Evid Based Complement Alternat Med 2022:1897508 (2022). PubMed: 36276866
- Kinoh H et al. Nanomedicines blocking adaptive signals in cancer cells overcome tumor TKI resistance. J Control Release 321:132-144 (2020). PubMed: 32032656
- Sheta R et al. Development of a 3D functional assay and identification of biomarkers, predictive for response of high-grade serous ovarian cancer (HGSOC) patients to poly-ADP ribose polymerase inhibitors (PARPis): targeted therapy. J Transl Med 18:439 (2020). PubMed: 33213473
- Lu YL et al. Co-delivery of Cyclopamine and Doxorubicin Mediated by Bovine Serum Albumin Nanoparticles Reverses Doxorubicin Resistance in Breast Cancer by Down-regulating P-glycoprotein Expression. J Cancer 10:2357-2368 (2019). PubMed: 31258739
- Jiang Y et al. The Optimization Design Of Lactoferrin Loaded HupA Nanoemulsion For Targeted Drug Transport Via Intranasal Route. Int J Nanomedicine 14:9217-9234 (2019). PubMed: 31819426
- Ma M et al. Celecoxib enhances sensitivity to chemotherapy drugs of T-cell lymphoma. Oncol Lett 15:4649-4656 (2018). PubMed: 29541237
- Reinhard FB et al. Thermal proteome profiling monitors ligand interactions with cellular membrane proteins. Nat Methods 12:1129-31 (2015). PubMed: 26524241
- Kang CC et al. Single-cell Western blotting after whole-cell imaging to assess cancer chemotherapeutic response. Anal Chem 86:10429-36 (2014). Human . PubMed: 25226230