重组Anti-P Glycoprotein抗体[EPR10364-57] - BSA and Azide free (ab216656)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10364-57] to P Glycoprotein - BSA and Azide free
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-P Glycoprotein抗体[EPR10364-57] - BSA and Azide free
参阅全部 P Glycoprotein 一抗 -
描述
兔单克隆抗体[EPR10364-57] to P Glycoprotein - BSA and Azide free -
宿主
Rabbit -
特异性
P-glycoprotein 1 (also known as Multidrug resistance protein 1) has a predicted molecular weight of 141 kDa, however it has 3 potential glycosylation sites (N-linked) which may affect the migration of the protein. In our hands this antibody detects a predominant protein band migrating in the region of 180-200 kDa and typically will demonstrate a smear on the membrane in the region of the 150 – 300 kDa due to the glycosylation profile of the protein. It may be necessary to optimise your cell or tissue lysis protocol to efficiently extract P-glycoprotein 1 as it is a multi-pass membrane protein. Abcam recommends not boiling the sample after lysis. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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经测试应用
适用于: WB, IHC-Pmore details
不适用于: ICC/IF -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment aa 350-750. The exact sequence is proprietary.
Database link: P08183 -
阳性对照
- WB: 293T, HeLa, HepG2 and 293T cell lysates; human fetal brain tissue lysate; mouse brain, heart, kidney and spleen tissue lysates; rat brain, heart, kidney and spleen tissue lysates. IHC-P: Human hepatocellular carcinoma, brain and kidney tissues.
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常规说明
ab216656 is the carrier-free version of ab170904.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.20
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR10364-57 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
- HeLa whole cell lysate (ab150035)
- Mouse spleen tissue lysate - total protein (ab29293)
- Mouse kidney normal tissue lysate - total protein (ab29305)
- HeLa whole cell lysate (ab29545)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse heart normal tissue lysate - total protein (ab30291)
- Mouse brain tissue lysate - total protein (0 days) (ab7188)
- Mouse brain tissue lysate - total protein (14 days) (ab7189)
- Mouse brain tissue lysate - total protein (7 days) (ab7190)
- Mouse heart tissue lysate - total protein (0 days) (ab7193)
- Mouse heart tissue lysate - total protein (14 days) (ab7194)
- Mouse kidney tissue lysate - total protein (0 days) (ab7261)
- Mouse kidney tissue lysate (14 days) (ab7262)
- Mouse kidney tissue lysate (7 days) (ab7263)
- Mouse spleen tissue lysate - total protein (14 days) (ab7274)
- Mouse spleen tissue lysate - total protein (ab7937)
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab216656于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 141 kDa.
For optimal detection Abcam recommends not boiling the sample after lysis. |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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说明 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 141 kDa. For optimal detection Abcam recommends not boiling the sample after lysis. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells. -
组织特异性
Expressed in liver, kidney, small intestine and brain. -
疾病相关
Genetic variations in ABCB1 are associated with susceptibility to inflammatory bowel disease type 13 (IBD13) [MIM:612244]. Inflammatory bowel disease is characterized by a chronic relapsing intestinal inflammation. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Crohn disease and ulcerative colitis are commonly classified as autoimmune diseases. -
序列相似性
Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 5243 Human
- Entrez Gene: 18669 Mouse
- Entrez Gene: 24646 Rat
- Omim: 171050 Human
- SwissProt: P08183 Human
- SwissProt: P06795 Mouse
- SwissProt: P43245 Rat
- Unigene: 489033 Human
see all -
别名
- ABC20 antibody
- ABCB1 antibody
- ATP binding cassette, sub family B (MDR/TAP), member 1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling P Glycoprotein with purified ab170904 at 1:1200 dilution (0.24 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0) ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Hematoxylinwas used as a counterstain.
Negative control: PBS instead of the primary antibody (inset).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170904).
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Immunohistochemical staining of paraffin embedded human kidney with purified ab170904 at a working dilution of 1/100. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. Counterstained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
PBS was used instead of the primary antibody as the negative control (inset).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170904).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labeling P Glycoprotein with purified ab170904 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody.
Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170904).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labeling P Glycoprotein with unpurified ab170904 at 1/20. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody.
Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170904).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis human kidney tissue labeling P Glycoprotein with unpurified ab170904 at 1/250 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170904).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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This IHC data was generated using the same anti-P Glycoprotein antibody clone, EPR10364-57, in a different buffer formulation (cat# ab170904).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling P Glycoprotein with unpurified ab170904 at 1/250 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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This WB data was generated using the same anti-P Glycoprotein antibody clone, EPR10364-57, in a different buffer formulation (cat# ab170904).
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: P Glycoprotein knockout HAP1 whole cell lysate (20 µg)
Lane 3: HepG2 whole cell lysate (20 µg)Lanes 1 - 3: Merged signal (red and green). Green - ab170904 observed at 160 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab170904 was shown to specifically react with P Glycoprotein when P Glycoprotein knockout samples were used. Wild-type and P Glycoprotein knockout samples were subjected to SDS-PAGE. Ab170904 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (7)
ab216656 被引用在 7 文献中.
- Fu Y et al. Connexin 43 Modulates the Cellular Resistance to Paclitaxel via Targeting ß-Tubulin in Triple-Negative Breast Cancer. Onco Targets Ther 13:5323-5335 (2020). PubMed: 32606750
- Niu YC et al. MicroRNA-654-3p enhances cisplatin sensitivity by targeting QPRT and inhibiting the PI3K/AKT signaling pathway in ovarian cancer cells. Exp Ther Med 20:1467-1479 (2020). PubMed: 32742380
- Wuxiao Z et al. MicroRNA-145 promotes the apoptosis of leukemic stem cells and enhances drug-resistant K562/ADM cell sensitivity to adriamycin via the regulation of ABCE1. Int J Mol Med 46:1289-1300 (2020). PubMed: 32945355
- Xie C et al. Circular RNA hsa_circ_0003496 Contributes to Tumorigenesis and Chemoresistance in Osteosarcoma Through Targeting (microRNA) miR-370/Krüppel-Like Factor 12 Axis. Cancer Manag Res 12:8229-8240 (2020). PubMed: 32982419
- Fu TG et al. miR-143 inhibits oncogenic traits by degrading NUAK2 in glioblastoma. Int J Mol Med 37:1627-35 (2016). WB ; Human . PubMed: 27081712
- Aikawa H et al. Visualizing spatial distribution of alectinib in murine brain using quantitative mass spectrometry imaging. Sci Rep 6:23749 (2016). IHC-P ; Mouse . PubMed: 27026287
- Ma X et al. Essential role for TrpC5-containing extracellular vesicles in breast cancer with chemotherapeutic resistance. Proc Natl Acad Sci U S A 111:6389-94 (2014). Flow Cyt . PubMed: 24733904