重组Anti-P Glycoprotein抗体[EPR10364-57] (ab170904)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10364-57] to P Glycoprotein
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-P Glycoprotein抗体[EPR10364-57]
参阅全部 P Glycoprotein 一抗 -
描述
兔单克隆抗体[EPR10364-57] to P Glycoprotein -
宿主
Rabbit -
特异性
P-glycoprotein 1 (also known as Multidrug resistance protein 1) has a predicted molecular weight of 141 kDa, however it has 3 potential glycosylation sites (N-linked) which may affect the migration of the protein. In our hands this antibody detects a predominant protein band migrating in the region of 180-200 kDa and typically will demonstrate a smear on the membrane in the region of the 150-300 kDa due to the glycosylation profile of the protein. It may be necessary to optimise your cell or tissue lysis protocol to efficiently extract P-glycoprotein 1 as it is a multi-pass membrane protein. Abcam recommends not boiling the sample after lysis. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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经测试应用
适用于: IHC-P, WBmore details
不适用于: ICC/IF -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment within Human P Glycoprotein aa 350-750. The exact sequence is proprietary.
Database link: P08183 -
阳性对照
- WB: HEK-293T, C6, HeLa, HepG2 and HEK-293T cell lysates; human fetal brain tissue lysate; mouse and rat brain and kidney tissue lysates. Wild-type HAP1 cell lysate; IHC-P: Human hepatocellular carcinoma, brain and kidney tissues.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR10364-57 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
- HeLa whole cell lysate (ab150035)
- Mouse spleen tissue lysate - total protein (ab29293)
- Mouse kidney normal tissue lysate - total protein (ab29305)
- HeLa whole cell lysate (ab29545)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse heart normal tissue lysate - total protein (ab30291)
- Mouse brain tissue lysate - total protein (0 days) (ab7188)
- Mouse brain tissue lysate - total protein (14 days) (ab7189)
- Mouse brain tissue lysate - total protein (7 days) (ab7190)
- Mouse heart tissue lysate - total protein (0 days) (ab7193)
- Mouse heart tissue lysate - total protein (14 days) (ab7194)
- Mouse kidney tissue lysate - total protein (0 days) (ab7261)
- Mouse kidney tissue lysate (14 days) (ab7262)
- Mouse kidney tissue lysate (7 days) (ab7263)
- Mouse spleen tissue lysate - total protein (14 days) (ab7274)
- Mouse spleen tissue lysate - total protein (ab7937)
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab170904于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P | (1) |
1/1200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/20 - 1/100 The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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WB | (4) |
1/1000 - 1/5000. Predicted molecular weight: 141 kDa.
For optimal detection Abcam recommends not boiling the sample after lysis. |
说明 |
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IHC-P
1/1200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/20 - 1/100 The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
|
WB
1/1000 - 1/5000. Predicted molecular weight: 141 kDa. For optimal detection Abcam recommends not boiling the sample after lysis. |
靶标
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功能
Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells. -
组织特异性
Expressed in liver, kidney, small intestine and brain. -
疾病相关
Genetic variations in ABCB1 are associated with susceptibility to inflammatory bowel disease type 13 (IBD13) [MIM:612244]. Inflammatory bowel disease is characterized by a chronic relapsing intestinal inflammation. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Crohn disease and ulcerative colitis are commonly classified as autoimmune diseases. -
序列相似性
Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 5243 Human
- Entrez Gene: 18669 Mouse
- Entrez Gene: 24646 Rat
- Omim: 171050 Human
- SwissProt: P08183 Human
- SwissProt: P06795 Mouse
- SwissProt: P43245 Rat
- Unigene: 489033 Human
see all -
别名
- ABC20 antibody
- ABCB1 antibody
- ATP binding cassette, sub family B (MDR/TAP), member 1 antibody
see all
图片
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Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: P Glycoprotein knockout HAP1 whole cell lysate (20 µg)
Lane 3: HepG2 whole cell lysate (20 µg)Lanes 1 - 3: Merged signal (red and green). Green - ab170904 observed at 160 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab170904 was shown to specifically react with P Glycoprotein when P Glycoprotein knockout samples were used. Wild-type and P Glycoprotein knockout samples were subjected to SDS-PAGE. ab170904 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10000 dilution respectively.
Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 0.1 µg/ml (purified)
Lanes 1 & 3 : C6 (Rat glial tumor glial cell) whole cell lysates prepared using RIPA lysis method
Lanes 2 & 4 : C6 (Rat glial tumor glial cell) whole cell lysates prepared using 1%SDS Hot lysis method
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 141 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST.
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Immunohistochemical staining of paraffin embedded human kidney with purified ab170904 at a working dilution of 1/100. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. Counterstained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
PBS was used instead of the primary antibody as the negative control (inset).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling P Glycoprotein with purified ab170904 at 1:1200 dilution (0.24 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0) ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Hematoxylinwas used as a counterstain.
Negative control: PBS instead of the primary antibody (inset).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labeling P Glycoprotein with unpurified ab170904 at 1/250 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis human kidney tissue labeling P Glycoprotein with unpurified ab170904 at 1/250 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/2000 dilution (Purified)
Lanes 1 & 3 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates prepared using RIPA lysis method
Lanes 2 & 4 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate prepared using 1% SDS hot lysis method
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 141 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST
Exposure time: Left image -10 seconds; Right image - 1 minute.
We suggest not to boil the sample after lysis.
For 1% SDS Hot Lysates preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).
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All lanes : Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/2000 dilution (Purified)
Lanes 1 & 3 : Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates prepared using RIPA lysis method
Lanes 2 & 4 : Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate prepared using 1%SDS lysis method
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 141 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST
Exposure time: Left image - 3 seconds; Right image - 10 seconds.
We suggest not to boil the sample after lysis.
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All lanes : Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/1000 dilution (unpurified)
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
Lane 3 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 4 : Human fetal brain tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1-3 : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Lane 4 : Standard HRP labeled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 141 kDa -
All lanes : Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/1000 dilution (purified)
Lane 1 : Mouse brain lysate
Lane 2 : C6 (Rat glial tumor cell line) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 141 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/2000 dilution (purified)
Lane 1 : HeLa (human cervix adenocarcinoma) whole cell lysate
Lane 2 : HepG2 (human hepatocellular carcinoma) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 141 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/200 dilution (unpurified)
Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 141 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
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All lanes : Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/1000 dilution (purified)
Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 141 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
-
All lanes : Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/1000 dilution (unpurified)
Lane 1 : Rat brain tissue lysate
Lane 2 : Rat heart tissue lysate
Lane 3 : Rat kidney tissue lysate
Lane 4 : Rat spleen tissue lysate
Predicted band size: 141 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes -
All lanes : Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/1000 dilution (unpurified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Mouse spleen tissue lysate
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG (H+L) at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 141 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labeling P Glycoprotein with unpurified ab170904 at 1/20. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody.
Counterstained with hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labeling P Glycoprotein with purified ab170904 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody.
Counterstained with hematoxylin.
数据表及文件
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SDS download
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Datasheet download
文献 (166)
ab170904 被引用在 166 文献中.
- Nabi D et al. Multidrug resistance transporter-1 dysfunction perturbs meiosis and Ca2+ homeostasis in oocytes. Reproduction 165:79-91 (2023). PubMed: 36215093
- Wang Q et al. Mechanisms Underlying the Differences in the Pharmacokinetics of Six Active Constituents of Huangqi Liuyi Decoction between Normal and Diabetic Nephropathy Mouse Models. Evid Based Complement Alternat Med 2022:2481654 (2022). PubMed: 36285162
- Wang M et al. Dihydrotanshinone I Inhibits the Proliferation and Growth of Oxaliplatin-Resistant Human HCT116 Colorectal Cancer Cells. Molecules 27:N/A (2022). PubMed: 36431875
- Morimoto-Kamata R et al. Cathepsin G-Induced Cell Aggregation of Breast Cancer MCF-7 Decreases Doxorubicin Sensitivity in a Hypoxia-Inducible Factor-Independent Mechanism. Biol Pharm Bull 45:1772-1783 (2022). PubMed: 36450530
- Duan Y et al. Exposure to High-Altitude Environment Is Associated with Drug Transporters Change: microRNA-873-5p-Mediated Alteration of Function and Expression Levels of Drug Transporters under Hypoxia. Drug Metab Dispos 50:174-186 (2022). PubMed: 34844996