Anti-Osteocalcin抗体[OCG4] (ab13421)


  • 产品名称
    参阅全部 Osteocalcin 一抗
  • 描述
    小鼠单克隆抗体[OCG4] to Osteocalcin
  • 宿主
  • 经测试应用
    适用于: ELISA, WB, IHC-Fr, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Sheep, Rabbit, Goat, Chicken, Cow, Dog, Human, Pig
    不与反应: Mouse, Rat
  • 免疫原

    Full length protein corresponding to Cow Osteocalcin.
    Database link: P02820

  • 表位
    Residues 21-31
  • 阳性对照
    • ICC/IF: SKNSH cells.
  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 



Our Abpromise guarantee covers the use of ab13421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1 - 1/10.
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 11 kDa.
IHC-Fr Use a concentration of 1 - 10 µg/ml.
IHC-P Use a concentration of 1 - 10 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 16820635


  • 功能
    Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
  • 序列相似性
    Belongs to the osteocalcin/matrix Gla protein family.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
  • 翻译后修饰
    Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
  • 细胞定位
  • Information by UniProt
  • 数据库链接
  • 别名
    • BGLAP antibody
    • BGP antibody
    • Bone gamma carboxyglutamate (gla) protein antibody
    • Bone gamma carboxyglutamate gla protein osteocalcin antibody
    • Bone gamma carboxyglutamate protein antibody
    • Bone Gla protein antibody
    • Gamma carboxyglutamic acid containing protein antibody
    • Gamma-carboxyglutamic acid-containing protein antibody
    • OC antibody
    • OCN antibody
    • OSTCN_HUMAN antibody
    • Osteocalcin antibody
    • OTTHUMP00000016586 antibody
    • PMF1 antibody
    see all


  • ICC/IF image of ab13421 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12421, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

  • Immunohistochemistry of human Synovial Sarcoma staining Osteocalcin with ab13421

  • Immunohistochemistry of human Synovial Sarcoma staining Osteocalcin with ab13421

  • Immunohistochemistry of human Chondroma staining Osteocalcin with ab13421


This product has been referenced in:
  • Zou L  et al. Angiogenic activity mediates bone repair from human pluripotent stem cell-derived osteogenic cells. Sci Rep 6:22868 (2016). IHC . Read more (PubMed: 26980556) »
  • González S  et al. A 3D culture system enhances the ability of human bone marrow stromal cells to support the growth of limbal stem/progenitor cells. Stem Cell Res 16:358-64 (2016). Read more (PubMed: 26896856) »

See all 6 Publications for this product


Gracias por contactarnos.

Tenemos en el catálogo, como bien indicas, tres anticuerpos contra Osteocalcina testados en humano mediante inmunocitoquímica e immunohistoquímica: ab13418, ab13420 y ab13421.

Todos nue...

Read More

Nous somme vraiment désolés de ne pas avoir pu vous fournir un bon anticorps anti-osteocalcine. J'ai demandé à notre service comptabilité de mettre en place un avoir référence CN.

Votre référence CN est *******.

Voici les man...

Read More

Merci d'avoir répondu à notre questionnaire.

Je suis désolé d'apprendre que vous rencontrez toujours des problèmes avec l'anticorps anti-osteocalcin ab13421 que je vous ai envoyé en remplacement du ab...

Read More

Thank you for your patience.

I have received the following information about the other 3 osteocalcin antibodies I refered to in my last email:

ab13418, ab13421: These antibodies were derived from mice that were immunized with ...

Read More

Thank you for your phone call.

I have checked our osteocalcin antibodies and found one where I know for sure that it was raised against the mature human protein without any modification: ab35078

Read More

That is correct. However, we do stand-by our Abpromise and so if you do have any problems with the antibody, then we would be happy to help out as much as possible and replace or refund the antibody if necessary. Please let me know if there is anything...

Read More

Thank you for contacting Abcam. The antibody ab13421 was deemed not to react with mouse and rat tissue because of sequence homology. The peptide used to generate the antibody (aa21-31 of Cow Osteocalcin) has a low level of similarity to the mouse and r...

Read More

Merci pour ces précisions. Nous sommes désolés d'apprendre que le produit ab13420 que vous avez reçu ne fonctionne pas comme attendu. Comme convenu,j'ai procédé à l'envoi d'une unité de remplaceme...

Read More

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab13421. To check the status of the order please contact our Customer...

Read More

I can confirm that proteinase K mediated antigen retrieval has already given positive results with this antibody.

1-10 of 11 Q&A