cross-reactivity with ospC from Borrelia burgdorferi, afzelii, and valaisiana sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against B. japonica and americana sources based on 94% homology
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 22 kDa.
Outer Surface Protein C, or ospC, is a 20.7 kDa immunogenic protein on
the outer surface of the spirochete Borrelia burgdorferi. Its function is not known, but it is located with lipid-anchoring sites on the outer cell
Cell outer membrane; Lipid-anchor.
Outer surface protein C antibody
Western blot - Anti-ospC antibody (ab106083)
All lanes : Anti-ospC antibody (ab106083) at 1/1000 dilution
Lane 1 : MBP-ospC fusion protein Lane 2 : MBP alone
Lysates/proteins at 0.1 µg per lane.
Secondary All lanes : Goat-anti-Rabbit HRP at 1/40000 dilution