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Neuroscience Neurotransmitter Neuropeptides Hormones

Anti-Orexin A抗体(ab6214)

  • Datasheet
  • SDS
Reviews (2)Q&A (1)References (6)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orexin A antibody (ab6214)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orexin A antibody (ab6214)

Key features and details

  • Rabbit polyclonal to Orexin A
  • Suitable for: IHC-P
  • Reacts with: Rat
  • Isotype: IgG

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概述

  • 产品名称

    Anti-Orexin A抗体
    参阅全部 Orexin A 一抗
  • 描述

    兔多克隆抗体to Orexin A
  • 宿主

    Rabbit
  • 特异性

    Immunohistochemical analysis of rat brain indicates a good specificity for this serum.
  • 经测试应用

    适用于: IHC-Pmore details
  • 种属反应性

    与反应: Rat
  • 免疫原

    Synthetic peptide corresponding to Cow Orexin A aa 1-100 conjugated to keyhole limpet haemocyanin (Glutaraldehyde).
    Database link: P56717

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7
  • Concentration information loading...
  • 纯度

    Whole antiserum
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Neurotransmitter
    • Neuropeptides
    • Hormones
    • Neuroscience
    • Neurology process
    • Metabolism
    • Metabolism
    • Types of disease
    • Obesity
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6214于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P (2)
Use at an assay dependent concentration.
说明
IHC-P
Use at an assay dependent concentration.

靶标

  • 相关性

    Orexin A (hypocretin 1) a 33 amino acid peptide and orexin B (hypocretin 2), a 28 amino acid peptide, are both derived from a common 130 amino acid precursor, prepro orexin. Orexin A and Orexin B stimulate food consumption when administered intracerebroventricularly to rats. Orexin gene expression in the brain is highly restricted to distinct populations of neurons located in specific hypothalamic regions, including the lateral hypothalamic area (LHA), a region implicated in feeding behaviour. Orexin A and orexin B bind to and activate two closely related G protein coupled receptors (GPCRs), termed Orexin 1 (OX1) and Orexin 2 (OX2) receptors. Dysfunction of the orexin peptide system has been linked to narcolepsy.
  • 细胞定位

    Rough endoplasmic reticulum. Cytoplasmic vesicle. Cell junction, synapse. Note: Associated with perikaryal rough endoplasmic reticulum as well as cytoplasmic large granular vesicles at synapses.
  • 数据库链接

    • Entrez Gene: 25723 Rat
    • SwissProt: O55232 Rat
    • 别名

      • HCRT antibody
      • HCRT1 antibody
      • HCRT2 antibody
      • hypocretin (orexin) neuropeptide precursor antibody
      • Hypocretin 1 antibody
      • Hypocretin 2 antibody
      • hypocretin antibody
      • NRCLP1 antibody
      • Orexin antibody
      • Orexin A antibody
      • Orexin B antibody
      • Orexin precursor antibody
      • OX antibody
      • PPORX antibody
      • PPOX antibody
      • PREPROOREXIN antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orexin A antibody (ab6214)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orexin A antibody (ab6214)

      Immunohistochemical staining of orexin A in rat lateral hypothalamus useing Anti-Orexin A antibody (ab6214) at 1:50000 dilution. The primary antibody was detected with the biotinylated secondary antibody-ABC method and nickel-diaminobenzidine chromogen.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orexin A antibody (ab6214)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orexin A antibody (ab6214)

      Immunohistochemical staining of orexin A in rat zona incerta neurons using Rabbit antibody to Orexin A (ab6214) at a dilution of 1 in 1000.

    实验方案

    • Immunohistochemistry protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (6)

    发表研究结果有使用 ab6214?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab6214 被引用在 6 文献中.

    • Wang Y  et al. Orexins alleviate motor deficits via increasing firing activity of pallidal neurons in a mouse model of Parkinson's disease. Am J Physiol Cell Physiol 317:C800-C812 (2019). PubMed: 31365289
    • Kakava-Georgiadou N  et al. Considerations related to the use of short neuropeptide promoters in viral vectors targeting hypothalamic neurons. Sci Rep 9:11146 (2019). PubMed: 31366942
    • Franco-Pérez J  et al. Chronic Consumption of Fructose Induces Behavioral Alterations by Increasing Orexin and Dopamine Levels in the Rat Brain. Nutrients 10:N/A (2018). PubMed: 30423806
    • Verma D  et al. Pancreatic polypeptide and its central Y4 receptors are essential for cued fear extinction and permanent suppression of fear. Br J Pharmacol 173:1925-38 (2016). IHC . PubMed: 26844810
    • Kerman IA  et al. Distinct populations of presympathetic-premotor neurons express orexin or melanin-concentrating hormone in the rat lateral hypothalamus. J Comp Neurol 505:586-601 (2007). PubMed: 17924541
    • Yao ST  et al. Water deprivation increases the expression of neuronal nitric oxide synthase (nNOS) but not orexin-A in the lateral hypothalamic area of the rat. J Comp Neurol 490:180-93 (2005). IHC-FoFr . PubMed: 16052497

    客户评价及客户问答

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    1-3 of 3 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Orexin A antibody

    Inconclusive
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (brain)
    Antigen retrieval step
    Heat mediated
    Specification
    brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Jul 15 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Orexin A antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    None
    Permeabilization
    Yes - Triton X-100 1%
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. Paloma Perez-Domper

    Verified customer

    提交于 Oct 27 2014

    Question

    We have bought from you the orexin A antibody (Orexin A - ab6214) which work in our system very well. We are going to publish the data. But since this antibody hasn't been characterized in the animal tissue we work with yet, we need to do the preabsorption control. Could you provide the free sample of the peptide so we will be able to do the control experiments. Also could you send us the suggestion concerning the preabsorptrion condition regarding this antibody. Thank you in advance for your help. Sincerely,

    Read More

    Abcam community

    Verified customer

    Asked on Oct 30 2002

    Answer

    Thank you for your enquiry regarding the peptide immunogen used to generate ab6214 -- Rabbit polyclonal to Orexin A. I am afraid that we do not have stocks of this peptide here at Abcam so I am unable to send you a free sample for your control experiments.

    Read More

    Abcam Scientific Support

    回复于 Nov 04 2002

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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