Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oligodendrocyte Specific Protein antibody (ab53041)This image is courtesy of an Abreview submitted by Carl Hobbs
ab53041 staining cat brain sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab53041 at 1/500 in TRIS/BSA/azide for 2h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-Oligodendrocyte Specific Protein antibody (ab53041)Image courtesy of an anonymous Abreview.
ab53041 staining Oligodendrocyte Specific Protein in mixed glia prepared from mouse brain by Immunocytochemistry/ Immunofluorescence. The cells were fixed in ice-cold methanol and then blocked using 20% serum with 0.5% Saponin for 1 hour at 4°C. Samples were then incubated with primary antibody at 1/200 for 2 hours at 23°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/400 dilution. DAPI was used to stain the cell nuclei (blue).
Immunohistochemistry (Frozen sections) - Anti-Oligodendrocyte Specific Protein antibody (ab53041)This image is courtesy of an Abreview submitted by Dr J Moffett
ab53041 diluted 1/500 in 2% normal goat serum staining rat brain tissue sections by IHC-Fr. The tissue was formaldehyde fixed and blocked with 2% serum for 30 minutes at 21°C before incubation with the primary antibody for 24 hours at 21°C. A biotinylated goat anti-rabbit antibody was used as the secondary.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oligodendrocyte Specific Protein antibody (ab53041)This image is a courtesy of Anonymous Abreview
ab53041 staining Oligodendrocyte Specific Protein in mouse brain tissue section by Immunohistochemistry (PFA-fixed). The tissue sections were fixed with 4% paraformaldehyde.The primary antibody was diluted 1/3000 and incubated with sample for 16 hours at 4°C in PBS 0.3% Triton 8% BSA. An Alexa Fluor® 568 conjugated goat polyclonal to rabbit IgG was used at dilution 1/1000 as secondary antibody. The image was taken from the cortex of an adult mouse, near the midline.
Li HP et al. Inactivation of the tight junction gene CLDN11 by aberrant hypermethylation modulates tubulins polymerization and promotes cell migration in nasopharyngeal carcinoma. J Exp Clin Cancer Res37:102 (2018).
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Luo L et al. Niche astrocytes promote the survival, proliferation and neuronal differentiation of co-transplanted neural stem cells following ischemic stroke in rats. Exp Ther Med13:645-650 (2017).
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