Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human embryonic stem cells)
Permeabilization
Yes - PBS/Triton X-100
Specification
human embryonic stem cells
Blocking step
Donkey serum/BSA (1%) as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Donkey serum/BSA in PBST
Dilution
1/250
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
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提交于 Jul 04 2017