Anti-O-Linked N-Acetylglucosamine抗体[RL2] (ab2739)
![Immunocytochemistry/ Immunofluorescence - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)](https://www.abcam.cn/ps/products/2/ab2739/Images/ab2739-279434-anti-o-linked-n-acetylglucosamine-antibody-rl2-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)")
Key features and details
- Mouse monoclonal [RL2] to O-Linked N-Acetylglucosamine
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG1
Related conjugates and formulations
概述
-
产品名称
Anti-O-Linked N-Acetylglucosamine抗体[RL2]
参阅全部 O-Linked N-Acetylglucosamine 一抗 -
描述
小鼠单克隆抗体[RL2] to O-Linked N-Acetylglucosamine -
宿主
Mouse -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Rat, Human -
免疫原
Tissue, cells or virus corresponding to O-Linked N-Acetylglucosamine. Specifically, isolated rat liver nuclear envelopes, which contain 8 O-Linked glycoproteins in the nuclear pore complex
-
阳性对照
- ICC-IF: MCF7 cells. WB: Jurkat cells treated with 50 uM PugNAc; SH-SY5Y) whole cell lysate - treated with 50µM z-Pugnac; Rat Liver Nuclear Envelope lysate.
-
常规说明
This antibody clone [RL2] is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
RL2 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
-
Alternative Versions
-
ChIP Related Products
-
Compatible Secondaries
-
Isotype control
-
Related Products
应用
靶标
-
相关性
Many cellular proteins, including nuclear pore, oncogene, cytoskeletal, heat shock, viral and transcription regulatory proteins contain single O-linked N-acetylglucosamine (O-GlcNAc) residues attached to serine or threonine residues. It has been observed that O-GlcNAc glycosylated proteins tend to be under phosphorylated relative to unglycosylated proteins and that O-GlcNAc bearing proteins tend to be found in multimeric complexes. This has led to the suggestion that O-GlcNAc glycosylation may obscure phosphorylation sites and acts as a signaling mechanism or mediator of signaling.
图片
-
Immunocytochemistry/ Immunofluorescence - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)
ab2739 stained in MCF7 cells. Cells were fixed with 4% paraformaldehyde (10min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab2739 at 5µg/ml and ab6046 (Rabbit polyclonal to beta Tubulin - Loading Control) at 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150080 (pseudo-colored red) and ab150117 (colored green) used at 1 ug/ml for 1hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43µM for 1hour at room temperature.
-
![Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)](https://www.abcam.cn/ps/products/2/ab2739/Images/ab2739-232645-anti-o-linked-n-acetylglucosamine-antibody-rl2-western-blot.jpg)
Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)All lanes : Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739) at 1 µg/ml
Lanes 1 & 3 : Jurkat cells treated with 0 uM PugNAc
Lane 2 : Jurkat cells treated with 50 uM PugNAc (3 hours)
Lane 4 : Jurkat cells treated with 4 mM glucosamine and 50 uM PugNAc (3 hours)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 12 minutesJurkat cells were treated with either 50 uM PugNAc (ab144670) or 4 mM glucosamine + 50 uM PugNAc (ab144670) for three hours prior to harvest to stimulate O-linked glycosylation. The expected increase in glycosylation is observed in the treated lanes 2 & 4.
-
![Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)](https://www.abcam.cn/ps/products/2/ab2739/Images/ab2739-232732-anti-o-linked-n-acetylglucosamine-antibody-rl2-western-blot.jpg)
Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)This image is courtesy of an anonymous AbreviewAll lanes : Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739) at 1/3000 dilution
Lane 1 : Human neuroblastoma (SH-SY5Y) whole cell lysate - treated with 50µM z-Pugnac for 24 hours
Lane 2 : Human neuroblastoma (SH-SY5Y) whole cell lysate - untreated
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated horse anti-mouse IgG polyclonal
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 30 seconds
-
![Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)](https://www.abcam.cn/ps/products/2/ab2739/Images/ab2739-6300-anti-o-linked-n-acetylglucosamine-antibody-rl2-western-blot.jpg)
Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739) at 1 µg/ml + Rat Liver Nuclear Envelope at 10 µg
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 1 minute
The antibody was tested against the immunogen (isolated rat liver nuclear envelopes, which contain 8 O-linked glycoproteins in the nuclear pore complex).
数据表及文件
-
SDS download
-
Datasheet download
文献 (136)
ab2739 被引用在 136 文献中.
- Erber LN et al. Iron Deficiency Reprograms Phosphorylation Signaling and Reduces O-GlcNAc Pathways in Neuronal Cells. Nutrients 13:N/A (2021). PubMed: 33430126
- Verathamjamras C et al. Aberrant RL2 O-GlcNAc antibody reactivity against serum-IgA1 of patients with colorectal cancer. Glycoconj J 38:55-65 (2021). PubMed: 33608772
- Shin EM et al. GREB1: An evolutionarily conserved protein with a glycosyltransferase domain links ERa glycosylation and stability to cancer. Sci Adv 7:N/A (2021). PubMed: 33731348
- Zhang X et al. Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma. Theranostics 11:5650-5674 (2021). PubMed: 33897873
- Ou W et al. Hypoxic acclimation improves cardiac redox homeostasis and protects heart against ischemia-reperfusion injury through upregulation of O-GlcNAcylation. Redox Biol 43:101994 (2021). PubMed: 33964586