Anti-O-Linked N-Acetylglucosamine抗体[RL2] (ab2739)

Reviews (16)Q&A (10)References (112)
Immunocytochemistry/ Immunofluorescence - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)
  • Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)
  • Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)
  • Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)

Key features and details

  • Mouse monoclonal [RL2] to O-Linked N-Acetylglucosamine
  • Suitable for: ICC/IF, WB
  • Reacts with: Rat, Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-O-Linked N-Acetylglucosamine抗体[RL2]
    参阅全部 O-Linked N-Acetylglucosamine 一抗

  • 描述

    小鼠单克隆抗体[RL2] to O-Linked N-Acetylglucosamine

  • 宿主

    Mouse

  • 经测试应用

    适用于: ICC/IF, WBmore details

  • 种属反应性

    与反应: Rat, Human

  • 免疫原

    Tissue, cells or virus corresponding to O-Linked N-Acetylglucosamine. Specifically, isolated rat liver nuclear envelopes, which contain 8 O-Linked glycoproteins in the nuclear pore complex

  • 阳性对照

    • ICC-IF: MCF7 cells. WB: Jurkat cells treated with 50 uM PugNAc; SH-SY5Y) whole cell lysate - treated with 50µM z-Pugnac; Rat Liver Nuclear Envelope lysate.

  • 常规说明

    This antibody clone [RL2] is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2739于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF (2)
Use a concentration of 5 - 10 µg/ml.
WB (14)
Use a concentration of 1 µg/ml.
说明
ICC/IF
Use a concentration of 5 - 10 µg/ml.
WB
Use a concentration of 1 µg/ml.

靶标

  • 相关性

    Many cellular proteins, including nuclear pore, oncogene, cytoskeletal, heat shock, viral and transcription regulatory proteins contain single O-linked N-acetylglucosamine (O-GlcNAc) residues attached to serine or threonine residues. It has been observed that O-GlcNAc glycosylated proteins tend to be under phosphorylated relative to unglycosylated proteins and that O-GlcNAc bearing proteins tend to be found in multimeric complexes. This has led to the suggestion that O-GlcNAc glycosylation may obscure phosphorylation sites and acts as a signaling mechanism or mediator of signaling.

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)
    Immunocytochemistry/ Immunofluorescence - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)

    ab2739 stained in MCF7 cells. Cells were fixed with 4% paraformaldehyde (10min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab2739 at 5µg/ml and ab6046 (Rabbit polyclonal to beta Tubulin - Loading Control) at 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150080 (pseudo-colored red) and ab150117 (colored green) used at 1 ug/ml for 1hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)
    Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)
    All lanes : Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739) at 1 µg/ml

    Lanes 1 & 3 : Jurkat cells treated with 0 uM PugNAc
    Lane 2 : Jurkat cells treated with 50 uM PugNAc (3 hours)
    Lane 4 : Jurkat cells treated with 4 mM glucosamine and 50 uM PugNAc (3 hours)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 12 minutes


    Jurkat cells were treated with either 50 uM PugNAc (ab144670) or 4 mM glucosamine + 50 uM PugNAc (ab144670) for three hours prior to harvest to stimulate O-linked glycosylation. The expected increase in glycosylation is observed in the treated lanes 2 & 4. 

  • Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)
    Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)This image is courtesy of an anonymous Abreview
    All lanes : Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739) at 1/3000 dilution

    Lane 1 : Human neuroblastoma (SH-SY5Y) whole cell lysate - treated with 50µM z-Pugnac for 24 hours
    Lane 2 : Human neuroblastoma (SH-SY5Y) whole cell lysate - untreated

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP-conjugated horse anti-mouse IgG polyclonal

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 30 seconds

    See Abreview

  • Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)
    Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)
    Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739) at 1 µg/ml + Rat Liver Nuclear Envelope at 10 µg

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 1 minute


    The antibody was tested against the immunogen (isolated rat liver nuclear envelopes, which contain 8 O-linked glycoproteins in the nuclear pore complex).

文献 (112)

发表研究结果有使用 ab2739？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab2739 被引用在 112 文献中.

  • Netsirisawan P  et al. Quantitative proteomic analysis of the association between decreasing O-GlcNAcylation and metastasis in MCF-7 breast cancer cells. Int J Oncol 56:1387-1404 (2020). PubMed: 32236627
  • Yang Y  et al. O-GlcNAc transferase inhibits visceral fat lipolysis and promotes diet-induced obesity. Nat Commun 11:181 (2020). PubMed: 31924761
  • Yu S  et al. The Histone Demethylase JMJD1C Regulates CAMKK2-AMPK Signaling to Participate in Cardiac Hypertrophy. Front Physiol 11:539 (2020). PubMed: 32625104
  • Jia C  et al. GFAT1/HBP/O-GlcNAcylation Axis Regulates ß-Catenin Activity to Promote Pancreatic Cancer Aggressiveness. Biomed Res Int 2020:1921609 (2020). PubMed: 32149084
  • Tan ZW  et al. O-GlcNAc regulates gene expression by controlling detained intron splicing. Nucleic Acids Res 48:5656-5669 (2020). PubMed: 32329777
View all Publications for this product

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1-2 of 2 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-O-Linked N-Acetylglucosamine antibody [RL2]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Liver)
Permeabilization
No
Specification
Liver
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

提交于 Nov 30 2019

Immunocytochemistry/ Immunofluorescence abreview for Anti-O-Linked N-Acetylglucosamine antibody [RL2]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS cells)
Permeabilization
Yes - NP40
Specification
U2OS cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

提交于 Sep 07 2018

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