Anti-O-Linked N-Acetylglucosamine抗体[RL2] (ab2739)
![Immunocytochemistry/ Immunofluorescence - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)](https://www.abcam.cn/ps/products/2/ab2739/Images/ab2739-279434-anti-o-linked-n-acetylglucosamine-antibody-rl2-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)")
Key features and details
- Mouse monoclonal [RL2] to O-Linked N-Acetylglucosamine
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG1
概述
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产品名称
Anti-O-Linked N-Acetylglucosamine抗体[RL2]
参阅全部 O-Linked N-Acetylglucosamine 一抗 -
描述
小鼠单克隆抗体[RL2] to O-Linked N-Acetylglucosamine -
宿主
Mouse -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Rat, Human -
免疫原
Tissue, cells or virus corresponding to O-Linked N-Acetylglucosamine. Specifically, isolated rat liver nuclear envelopes, which contain 8 O-Linked glycoproteins in the nuclear pore complex
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阳性对照
- ICC-IF: MCF7 cells. WB: Jurkat cells treated with 50 uM PugNAc; SH-SY5Y) whole cell lysate - treated with 50µM z-Pugnac; Rat Liver Nuclear Envelope lysate.
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常规说明
This antibody clone [RL2] is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
纯度
IgG fraction -
克隆
单克隆 -
克隆编号
RL2 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Related Products
应用
Our Abpromise guarantee covers the use of ab2739 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use a concentration of 5 - 10 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. |
靶标
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相关性
Many cellular proteins, including nuclear pore, oncogene, cytoskeletal, heat shock, viral and transcription regulatory proteins contain single O-linked N-acetylglucosamine (O-GlcNAc) residues attached to serine or threonine residues. It has been observed that O-GlcNAc glycosylated proteins tend to be under phosphorylated relative to unglycosylated proteins and that O-GlcNAc bearing proteins tend to be found in multimeric complexes. This has led to the suggestion that O-GlcNAc glycosylation may obscure phosphorylation sites and acts as a signaling mechanism or mediator of signaling.
图片
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Immunocytochemistry/ Immunofluorescence - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)
ab2739 stained in MCF7 cells. Cells were fixed with 4% paraformaldehyde (10min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab2739 at 5µg/ml and ab6046 (Rabbit polyclonal to beta Tubulin - Loading Control) at 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150080 (pseudo-colored red) and ab150117 (colored green) used at 1 ug/ml for 1hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43µM for 1hour at room temperature.
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![Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)](https://www.abcam.cn/ps/products/2/ab2739/Images/ab2739-232645-anti-o-linked-n-acetylglucosamine-antibody-rl2-western-blot.jpg)
Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)All lanes : Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739) at 1 µg/ml
Lanes 1 & 3 : Jurkat cells treated with 0 uM PugNAc
Lane 2 : Jurkat cells treated with 50 uM PugNAc (3 hours)
Lane 4 : Jurkat cells treated with 4 mM glucosamine and 50 uM PugNAc (3 hours)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 12 minutesJurkat cells were treated with either 50 uM PugNAc (ab144670) or 4 mM glucosamine + 50 uM PugNAc (ab144670) for three hours prior to harvest to stimulate O-linked glycosylation. The expected increase in glycosylation is observed in the treated lanes 2 & 4.
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![Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)](https://www.abcam.cn/ps/products/2/ab2739/Images/ab2739-232732-anti-o-linked-n-acetylglucosamine-antibody-rl2-western-blot.jpg)
Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)This image is courtesy of an anonymous AbreviewAll lanes : Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739) at 1/3000 dilution
Lane 1 : Human neuroblastoma (SH-SY5Y) whole cell lysate - treated with 50µM z-Pugnac for 24 hours
Lane 2 : Human neuroblastoma (SH-SY5Y) whole cell lysate - untreated
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated horse anti-mouse IgG polyclonal
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 30 seconds
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![Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)](https://www.abcam.cn/ps/products/2/ab2739/Images/ab2739-6300-anti-o-linked-n-acetylglucosamine-antibody-rl2-western-blot.jpg)
Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739) at 1 µg/ml + Rat Liver Nuclear Envelope at 10 µg
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 1 minute
The antibody was tested against the immunogen (isolated rat liver nuclear envelopes, which contain 8 O-linked glycoproteins in the nuclear pore complex).
文献 (110)
ab2739 被引用在 110 文献中.
- Netsirisawan P et al. Quantitative proteomic analysis of the association between decreasing O-GlcNAcylation and metastasis in MCF-7 breast cancer cells. Int J Oncol 56:1387-1404 (2020). PubMed: 32236627
- Yang Y et al. O-GlcNAc transferase inhibits visceral fat lipolysis and promotes diet-induced obesity. Nat Commun 11:181 (2020). PubMed: 31924761
- Jia C et al. GFAT1/HBP/O-GlcNAcylation Axis Regulates ß-Catenin Activity to Promote Pancreatic Cancer Aggressiveness. Biomed Res Int 2020:1921609 (2020). PubMed: 32149084
- Tan ZW et al. O-GlcNAc regulates gene expression by controlling detained intron splicing. Nucleic Acids Res 48:5656-5669 (2020). PubMed: 32329777
- Yu S et al. The Histone Demethylase JMJD1C Regulates CAMKK2-AMPK Signaling to Participate in Cardiac Hypertrophy. Front Physiol 11:539 (2020). PubMed: 32625104
