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Anti-O-Linked N-Acetylglucosamine抗体[RL2] (ab2739)

  • Datasheet
  • SDS
Reviews (15)Q&A (10)References (110)

Product price, shipping and contact information

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Immunocytochemistry/ Immunofluorescence - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)
  • Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)
  • Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)
  • Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)

Key features and details

  • Mouse monoclonal [RL2] to O-Linked N-Acetylglucosamine
  • Suitable for: ICC/IF, WB
  • Reacts with: Rat, Human
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-O-Linked N-Acetylglucosamine抗体[RL2]
    参阅全部 O-Linked N-Acetylglucosamine 一抗
  • 描述

    小鼠单克隆抗体[RL2] to O-Linked N-Acetylglucosamine
  • 宿主

    Mouse
  • 经测试应用

    适用于: ICC/IF, WBmore details
  • 种属反应性

    与反应: Rat, Human
  • 免疫原

    Tissue, cells or virus corresponding to O-Linked N-Acetylglucosamine. Specifically, isolated rat liver nuclear envelopes, which contain 8 O-Linked glycoproteins in the nuclear pore complex

  • 阳性对照

    • ICC-IF: MCF7 cells. WB: Jurkat cells treated with 50 uM PugNAc; SH-SY5Y) whole cell lysate - treated with 50µM z-Pugnac; Rat Liver Nuclear Envelope lysate.
  • 常规说明

    This antibody clone [RL2] is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • 纯度

    IgG fraction
  • 克隆

    单克隆
  • 克隆编号

    RL2
  • 同种型

    IgG1
  • 轻链类型

    kappa
  • 研究领域

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Signal Transduction
    • Protein Trafficking
    • Nuclear Import / Export
    • Cell Biology
    • Other Antibodies
    • Other Antibodies

相关产品

  • Alternative Versions

    • Alexa Fluor® 488 Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab201993)
    • Alexa Fluor® 647 Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab201994)
    • HRP Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab201995)
    • Anti-O-Linked N-Acetylglucosamine antibody [RL2] - BSA and Azide free (ab264496)
  • ChIP Related Products

    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Related Products

    • Tunicamycin, Protein glycosylation inhibitor (ab120296)

应用

Our Abpromise guarantee covers the use of ab2739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 - 10 µg/ml.
WB Use a concentration of 1 µg/ml.

靶标

  • 相关性

    Many cellular proteins, including nuclear pore, oncogene, cytoskeletal, heat shock, viral and transcription regulatory proteins contain single O-linked N-acetylglucosamine (O-GlcNAc) residues attached to serine or threonine residues. It has been observed that O-GlcNAc glycosylated proteins tend to be under phosphorylated relative to unglycosylated proteins and that O-GlcNAc bearing proteins tend to be found in multimeric complexes. This has led to the suggestion that O-GlcNAc glycosylation may obscure phosphorylation sites and acts as a signaling mechanism or mediator of signaling.

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)
    Immunocytochemistry/ Immunofluorescence - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)

    ab2739 stained in MCF7 cells. Cells were fixed with 4% paraformaldehyde (10min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab2739 at 5µg/ml and ab6046 (Rabbit polyclonal to beta Tubulin - Loading Control) at 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150080 (pseudo-colored red) and ab150117 (colored green) used at 1 ug/ml for 1hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)
    Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)
    All lanes : Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739) at 1 µg/ml

    Lanes 1 & 3 : Jurkat cells treated with 0 uM PugNAc
    Lane 2 : Jurkat cells treated with 50 uM PugNAc (3 hours)
    Lane 4 : Jurkat cells treated with 4 mM glucosamine and 50 uM PugNAc (3 hours)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 12 minutes


    Jurkat cells were treated with either 50 uM PugNAc (ab144670) or 4 mM glucosamine + 50 uM PugNAc (ab144670) for three hours prior to harvest to stimulate O-linked glycosylation. The expected increase in glycosylation is observed in the treated lanes 2 & 4. 

  • Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)
    Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)This image is courtesy of an anonymous Abreview
    All lanes : Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739) at 1/3000 dilution

    Lane 1 : Human neuroblastoma (SH-SY5Y) whole cell lysate - treated with 50µM z-Pugnac for 24 hours
    Lane 2 : Human neuroblastoma (SH-SY5Y) whole cell lysate - untreated

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP-conjugated horse anti-mouse IgG polyclonal

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 30 seconds

    See Abreview

  • Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)
    Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)
    Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739) at 1 µg/ml + Rat Liver Nuclear Envelope at 10 µg

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 1 minute


    The antibody was tested against the immunogen (isolated rat liver nuclear envelopes, which contain 8 O-linked glycoproteins in the nuclear pore complex).

实验方案

  • ChIP protocols

Click here to view the general protocols

数据表及文件

    • Datasheet
    • SDS
  • 文献 (110)

    发表研究结果有使用 ab2739?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2739 被引用在 110 文献中.

    • Netsirisawan P  et al. Quantitative proteomic analysis of the association between decreasing O-GlcNAcylation and metastasis in MCF-7 breast cancer cells. Int J Oncol 56:1387-1404 (2020). PubMed: 32236627
    • Yang Y  et al. O-GlcNAc transferase inhibits visceral fat lipolysis and promotes diet-induced obesity. Nat Commun 11:181 (2020). PubMed: 31924761
    • Jia C  et al. GFAT1/HBP/O-GlcNAcylation Axis Regulates ß-Catenin Activity to Promote Pancreatic Cancer Aggressiveness. Biomed Res Int 2020:1921609 (2020). PubMed: 32149084
    • Tan ZW  et al. O-GlcNAc regulates gene expression by controlling detained intron splicing. Nucleic Acids Res 48:5656-5669 (2020). PubMed: 32329777
    • Yu S  et al. The Histone Demethylase JMJD1C Regulates CAMKK2-AMPK Signaling to Participate in Cardiac Hypertrophy. Front Physiol 11:539 (2020). PubMed: 32625104
    View all Publications for this product

    客户评价及客户问答

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    提交评价 提交问题

    1-10 of 25 Abreviews or Q&A

    Western blot abreview for Anti-O-Linked N-Acetylglucosamine antibody [RL2]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (RAW264.7)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    5 µg
    Specification
    RAW264.7
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Oct 30 2020

    Western blot abreview for Anti-O-Linked N-Acetylglucosamine antibody [RL2]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Liver)
    Gel Running Conditions
    Non-reduced Denaturing (10% gel)
    Loading amount
    30 µg
    Specification
    Liver
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Nov 30 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-O-Linked N-Acetylglucosamine antibody [RL2]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Liver)
    Permeabilization
    No
    Specification
    Liver
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Nov 30 2019

    Western blot abreview for Anti-O-Linked N-Acetylglucosamine antibody [RL2]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Gel Running Conditions
    Reduced Denaturing (4-20% BioRad gel)
    Loading amount
    22 µg
    Treatment
    25 uM OSMI-1, 50 uM OSMI-1, 1 uM Thiamet G, 10 uM Thiamet G, 100 uM Thiamet G
    Specification
    HeLa
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    提交于 Oct 31 2019

    Western blot abreview for Anti-O-Linked N-Acetylglucosamine antibody [RL2]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (293T)
    Gel Running Conditions
    Reduced Denaturing (7.5% gel)
    Loading amount
    100000 cells
    Treatment
    1. 1.5g/L Glucose 2. 4.5 g/L Glucose for 24hrs
    Specification
    293T
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Aug 26 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-O-Linked N-Acetylglucosamine antibody [RL2]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (U2OS cells)
    Permeabilization
    Yes - NP40
    Specification
    U2OS cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Sep 07 2018

    Western blot abreview for Anti-O-Linked N-Acetylglucosamine antibody [RL2]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Vascular smooth muscle cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    25 µg
    Treatment
    100 uM H2O2 for 2 hours
    Specification
    Vascular smooth muscle cells
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    提交于 Feb 12 2018

    Western blot abreview for Anti-O-Linked N-Acetylglucosamine antibody [RL2]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (mast cell type)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native)
    Loading amount
    10 µg
    Specification
    mast cell type
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jun 01 2016

    Western blot abreview for Anti-O-Linked N-Acetylglucosamine antibody [RL2]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Bel-7402 cells)
    Gel Running Conditions
    Non-reduced Denaturing (gel 10%)
    Loading amount
    60 µg
    Specification
    Bel-7402 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Dr. guoqing zhu

    Verified customer

    提交于 Jan 20 2016

    Western blot abreview for Anti-O-Linked N-Acetylglucosamine antibody [RL2]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (8%)
    Sample
    Human Cell lysate - whole cell (T lymphocyte (human))
    Specification
    T lymphocyte (human)
    Treatment
    10,20,50 mM D-Galactopyranosyl-b-D-thiogalactopyranoside for 1hr
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    提交于 Feb 12 2015

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