重组Anti-NUP133抗体[EPR10808(B)] (ab155990)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10808(B)] to NUP133
- Suitable for: WB, ICC/IF
- Reacts with: Human, African green monkey
Related conjugates and formulations
概述
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产品名称
Anti-NUP133抗体[EPR10808(B)]
参阅全部 NUP133 一抗 -
描述
兔单克隆抗体[EPR10808(B)] to NUP133 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details
不适用于: Flow Cyt or IHC-P -
种属反应性
与反应: Human, African green monkey
预测可用于: Monkey -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa, Ramos and SH-SY5Y cell lysates; HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR10808(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab155990于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 129 kDa.
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ICC/IF | (1) |
1/50 - 1/100.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 129 kDa. |
ICC/IF
1/50 - 1/100. |
靶标
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功能
Involved in poly(A)+ RNA transport. -
序列相似性
Belongs to the nucleoporin Nup133 family. -
细胞定位
Nucleus > nuclear pore complex. Chromosome > centromere > kinetochore. Located on both the cytoplasmic and nuclear sides of the nuclear pore. During mitosis, localizes to the kinetochores. - Information by UniProt
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数据库链接
- Entrez Gene: 55746 Human
- Omim: 607613 Human
- SwissProt: Q8WUM0 Human
- Unigene: 12457 Human
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别名
- 133 kDa nucleoporin antibody
- FLJ10814 antibody
- hNUP 133 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling NUP133 with purified ab155990 at 1:50 (6.5 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Anti-NUP133 antibody [EPR10808(B)] (ab155990) at 1/1000 dilution (purified) + COS-1 (African green monkey kidney fibroblast-like) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 129 kDa
Observed band size: 129 kDa -
Lanes 1 & 3 : Anti-NUP133 antibody [EPR10808(B)] (ab155990) at 1/3000 dilution (purified)
Lane 2 : Anti-NUP133 antibody [EPR10808(B)] (ab155990) at 1/3000 dilution (purifed)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysates
Lane 3 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 129 kDa
Observed band size: 129 kDa -
All lanes : Anti-NUP133 antibody [EPR10808(B)] (ab155990) at 1/1000 dilution (unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : Ramos cell lysate
Lane 3 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 129 kDaThis image was produced using unpurified antibody.
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Unpurified ab155990 staining NUP133 in monkey kidney cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 3% BSA + 0.5% Triton X-100 for 45 minutes at 25°C. Samples were incubated with primary antibody (1/1500 in 3% BSA + 0.5% Triton X-100) for 45 minutes at 25°C. An Alexa Fluor® 647-conjugated donkey anti-rabbit IgG polyclonal (2 µg/ml) was used as the secondary antibody. This image was produced using unpurified antibody.
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Immunofluorescent analysis of HeLa cells labeling NUP133 with unpurified ab155990 at 1/50 dilution. This image was produced using unpurified antibody.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab155990 被引用在 7 文献中.
- Mammel AE et al. Chromosome length and gene density contribute to micronuclear membrane stability. Life Sci Alliance 5:N/A (2022). PubMed: 34789512
- Chachoua I et al. Canonical WNT signaling-dependent gating of MYC requires a noncanonical CTCF function at a distal binding site. Nat Commun 13:204 (2022). PubMed: 35017527
- Chou YY et al. Inherited nuclear pore substructures template post-mitotic pore assembly. Dev Cell 56:1786-1803.e9 (2021). PubMed: 34129835
- Bilir S et al. Roles of Nup133, Nup153 and membrane fenestrations in assembly of the nuclear pore complex at the end of mitosis. Genes Cells 24:338-353 (2019). PubMed: 30821042
- Platani M et al. Seh1 targets GATOR2 and Nup153 to mitotic chromosomes. J Cell Sci 131:N/A (2018). PubMed: 29618633
- Aristov A et al. ZOLA-3D allows flexible 3D localization microscopy over an adjustable axial range. Nat Commun 9:2409 (2018). PubMed: 29921892
- Liu S et al. Nuclear envelope assembly defects link mitotic errors to chromothripsis. Nature 561:551-555 (2018). PubMed: 30232450