Application
Western blot
Sample
Zebrafish Tissue lysate - whole (5 days whole tissue)
Gel Running Conditions
Reduced Denaturing (4–20% precast polyacrylamide gel)
Loading amount
30 µg
Specification
5 days whole tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Rabbit Anti-Goat Immunoglobulins/HRP (Dako)
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 90 kDa
Additional data
Additional Notes
observed specific band about 90 kDa, expected at 73 kDa but seems very specific and good
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jul 08 2021