Key features and details
- Goat polyclonal to NUMB
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
参阅全部 NUMB 一抗
特异性This antibody is expected to recognize all reported isoforms (NP_001005743.1, 001005744.1, NP_003735, NP_001005745.1). It is also likely to cross-react with Numblike (NUMBL, GeneID: 9253) which has a very similar C-Terminus, although this has not yet been tested experimentally.
经测试应用适用于: IHC-P, WB, ICC/IFmore details
种属反应性与反应: Mouse, Human
预测可用于: Chicken, Cow, Dog
- WB: Mouse brain lysate; ICC: A431 and NIH3T3 cells; IHC-P: Human prostate tissue.
常规说明This product may cross-react with Numblike (NUMBL, GeneID: 9253) Principal Names - NUMB; S171; numb homolog (Drosophila) Official Gene Symbol - NUMB GenBank Accession Number – NP_003735 LocusLink ID - 8650 (human); 18222 (mouse) Gene Ontology terms - integral to plasma membrane
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
纯度Immunogen affinity purified
纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab4147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 71 kDa).
1 hour primary incubation at room temperature is recommended for this product.
|ICC/IF||Use a concentration of 10 µg/ml.|
功能Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. May also mediate local repair of brain ventricular wall damage.
序列相似性Contains 1 PID domain.
翻译后修饰Isoform 1 and isoform 2 are ubiquitinated by LNX leading to their subsequent proteasomal degradation (By similarity). Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
- Information by UniProt
- c14_5527 antibody
- C14orf41 antibody
- FLJ31314 antibody
Anti-NUMB antibody (ab4147) at 1 µg/ml + Mouse brain lysates at 35 µg
Predicted band size: 71 kDa
Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence analysis of A431 cells labeling NUMB with ab4147 at 10ug/mL. Alexa Fluor488 at 2ug/mL was used as the secondary antibody. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/mL) followed by Alexa Fluor488 secondary antibody (2ug/mL).Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton.
Immunocytochemistry/ Immunofluorescence analysis of NIH3T3 cells labeling NUMB with ab4147 at 10ug/mL. Alexa Fluor488 at 2ug/mL was used as the secondary antibody. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/mL) followed by Alexa Fluor488 secondary antibody (2ug/mL).
Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton.
ab4147 staining NUMB in the NIH3T3 cell line from Mouse fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 10% serum for 60 minutes at 24°C. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit polyclonal(1/500) was used as the secondary antibody.
Single collagenase-isolated mouse muscle fibre stained with DAPI (blue) to reveal nuclei and anti-Numb (green) which detects satellite cells.
This picture was kindly supplied as part of the review submitted by Christopher Mann.
ab4147 被引用在 32 文献中.
- Li Y et al. CUL4B contributes to cancer stemness by repressing tumor suppressor miR34a in colorectal cancer. Oncogenesis 9:20 (2020). PubMed: 32054830
- Zhao Y et al. CD82-TRPM7-Numb signaling mediates age-related cognitive impairment. Geroscience 42:595-611 (2020). PubMed: 32088828
- Huang G et al. TUSC7 suppression of Notch activation through sponging MiR-146 recapitulated the asymmetric cell division in lung adenocarcinoma stem cells. Life Sci 232:116630 (2019). PubMed: 31279783
- Abdi K et al. EGFR Signaling Termination via Numb Trafficking in Ependymal Progenitors Controls Postnatal Neurogenic Niche Differentiation. Cell Rep 28:2012-2022.e4 (2019). PubMed: 31433979
- Cheng Y et al. m6A RNA Methylation Maintains Hematopoietic Stem Cell Identity and Symmetric Commitment. Cell Rep 28:1703-1716.e6 (2019). PubMed: 31412241