Anti-NUMB抗体(ab4147)
Key features and details
- Goat polyclonal to NUMB
- Suitable for: ELISA, IHC-P, IP, WB, ICC/IF
- Reacts with: Mouse, Chicken, Human
- Isotype: IgG
概述
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产品名称
Anti-NUMB抗体
参阅全部 NUMB 一抗 -
描述
山羊多克隆抗体to NUMB -
宿主
Goat -
特异性
This antibody is expected to recognize all reported isoforms (NP_001005743.1, 001005744.1, NP_003735, NP_001005745.1). It is also likely to cross-react with Numblike (NUMBL, GeneID: 9253) which has a very similar C-Terminus, although this has not yet been tested experimentally. -
经测试应用
适用于: ELISA, IHC-P, IP, WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Chicken, Human
预测可用于: Cow, Dog
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免疫原
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阳性对照
- WB: Mouse brain lysate; ICC: A431 and NIH3T3 cells;
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常规说明
This product may cross-react with Numblike (NUMBL, GeneID: 9253) Principal Names - NUMB; S171; numb homolog (Drosophila) Official Gene Symbol - NUMB GenBank Accession Number – NP_003735 LocusLink ID - 8650 (human); 18222 (mouse) Gene Ontology terms - integral to plasma membraneThe Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| IHC-P | (3) |
Use at an assay dependent concentration.
See Abreview. |
| IP | (1) |
Use at an assay dependent concentration.
For 1 hr on mouse brain lysate (see Abreview). |
| WB | (3) |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 71 kDa).
1 hour primary incubation at room temperature is recommended for this product. |
| ICC/IF | (3) |
Use a concentration of 10 µg/ml.
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| 说明 |
|---|
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ELISA
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. See Abreview. |
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IP
Use at an assay dependent concentration. For 1 hr on mouse brain lysate (see Abreview). |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 71 kDa). 1 hour primary incubation at room temperature is recommended for this product. |
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ICC/IF
Use a concentration of 10 µg/ml. |
靶标
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功能
Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. May also mediate local repair of brain ventricular wall damage. -
序列相似性
Contains 1 PID domain. -
翻译后修饰
Isoform 1 and isoform 2 are ubiquitinated by LNX leading to their subsequent proteasomal degradation (By similarity). Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 512187 Cow
- Entrez Gene: 480381 Dog
- Entrez Gene: 8650 Human
- Entrez Gene: 18222 Mouse
- Omim: 603728 Human
- SwissProt: P49757 Human
- SwissProt: Q9QZS3 Mouse
- Unigene: 654609 Human
see all -
别名
- c14_5527 antibody
- C14orf41 antibody
- FLJ31314 antibody
see all
图片
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Western blot - Anti-NUMB antibody (ab4147)Anti-NUMB antibody (ab4147) at 1 µg/ml + Mouse brain lysates at 35 µg
Predicted band size: 71 kDaDetected by chemiluminescence.
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Immunocytochemistry/ Immunofluorescence - Anti-NUMB antibody (ab4147)Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cell junction and cytoplasmic staining. The nuclear stain is DAPI (blue).
Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).
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Immunocytochemistry/ Immunofluorescence - Anti-NUMB antibody (ab4147)Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue).
Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).
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Immunocytochemistry/ Immunofluorescence - Anti-NUMB antibody (ab4147)This image is courtesy of an anonymous Abreviewab4147 staining NUMB in the NIH3T3 cell line from Mouse fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 10% serum for 60 minutes at 24°C. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit polyclonal(1/500) was used as the secondary antibody.
数据表及文件
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SDS download
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Datasheet download
文献 (39)
ab4147 被引用在 39 文献中.
- Charruyer A et al. Decreased p53 is associated with a decline in asymmetric stem cell self-renewal in aged human epidermis. Aging Cell 20:e13310 (2021). PubMed: 33524216
- Lau EO et al. DIAPH3 deficiency links microtubules to mitotic errors, defective neurogenesis, and brain dysfunction. Elife 10:N/A (2021). PubMed: 33899739
- Gomez K et al. Non-SUMOylated CRMP2 decreases NaV1.7 currents via the endocytic proteins Numb, Nedd4-2 and Eps15. Mol Brain 14:20 (2021). PubMed: 33478555
- Höpner SS et al. LIGHT/LTßR signaling regulates self-renewal and differentiation of hematopoietic and leukemia stem cells. Nat Commun 12:1065 (2021). PubMed: 33594067
- Li Y et al. CUL4B contributes to cancer stemness by repressing tumor suppressor miR34a in colorectal cancer. Oncogenesis 9:20 (2020). PubMed: 32054830