概述

  • 产品名称
    Anti-NUMB抗体
    参阅全部 NUMB 一抗
  • 描述
    山羊多克隆抗体to NUMB
  • 宿主
    Goat
  • 特异性
    This antibody is expected to recognize all reported isoforms (NP_001005743.1, 001005744.1, NP_003735, NP_001005745.1). It is also likely to cross-react with Numblike (NUMBL, GeneID: 9253) which has a very similar C-Terminus, although this has not yet been tested experimentally.
  • 经测试应用
    适用于: IHC-P, WB, IP, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Chicken, Human
    预测可用于: Cow, Dog
  • 免疫原

    Synthetic peptide: PFSSDLQKTFEIEL, corresponding to C terminal amino acids 638-651 of Human NUMB.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照
    • A431 lysate. Mouse brain lysates.
  • 常规说明
    This product may cross-react with Numblike (NUMBL, GeneID: 9253) Principal Names - NUMB; S171; numb homolog (Drosophila) Official Gene Symbol - NUMB GenBank Accession Number – NP_003735 LocusLink ID - 8650 (human); 18222 (mouse) Gene Ontology terms - integral to plasma membrane

性能

应用

Our Abpromise guarantee covers the use of ab4147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration. See Abreview
WB Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 71 kDa).Can be blocked with Human NUMB peptide (ab22990).
IP 1/1000. For 1 hr on mouse brain lysate (see Abreview).
ICC/IF Use at an assay dependent dilution. PubMed: 17127749

靶标

  • 功能
    Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. May also mediate local repair of brain ventricular wall damage.
  • 序列相似性
    Contains 1 PID domain.
  • 翻译后修饰
    Isoform 1 and isoform 2 are ubiquitinated by LNX leading to their subsequent proteasomal degradation (By similarity). Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • c14_5527 antibody
    • C14orf41 antibody
    • FLJ31314 antibody
    • h Numb antibody
    • h-Numb antibody
    • Numb antibody
    • Numb homolog (Drosophila) antibody
    • Numb homolog antibody
    • Numb protein homolog antibody
    • NUMB_HUMAN antibody
    • Protein numb homolog antibody
    • Protein S171 antibody
    • S171 antibody
    see all

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-NUMB antibody (ab4147)
    Immunocytochemistry/ Immunofluorescence - Anti-NUMB antibody (ab4147)This image is courtesy of an anonymous Abreview

    ab4147 staining NUMB in the NIH3T3 cell line from Mouse fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 10% serum for 60 minutes at 24°C. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. An  Alexa Fluor®488-conjugated Donkey anti-rabbit polyclonal(1/500) was used as the secondary antibody.

    See Abreview

  • Western blot - Anti-NUMB antibody (ab4147)
    Western blot - Anti-NUMB antibody (ab4147)

    Western Blot analysis of rat brain lysate (35µg protein in RIPA buffer)labeling NUMB with ab4147 at 0.01µg/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUMB antibody (ab4147)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUMB antibody (ab4147)

    Single collagenase-isolated mouse muscle fibre stained with DAPI (blue) to reveal nuclei and anti-Numb (green) which detects satellite cells.

    This picture was kindly supplied as part of the review submitted by Christopher Mann.

  • Western blot - Anti-NUMB antibody (ab4147)
    Western blot - Anti-NUMB antibody (ab4147)

    ab4147 staining (0.03µg/ml) of Mouse Brain lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

文献

This product has been referenced in:

See all 20 Publications for this product

发表研究结果有使用 ab4147？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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14 reviews or Q&A

10
Votes: Highest First

Immunocytochemistry/ Immunofluorescence abreview for Anti-NUMB antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH3T3)
Specification
NIH3T3
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
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Abcam user community

Verified customer

提交于 Mar 19 2013

Anti-NUMB antibody for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (Human)
Sample Species: Human
Type: Tissue sections
Specification: Skin equivalent
Fixative: Paraformaldehyde
Antigen retrieval step: H...

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Thank you for submitting an Abreview of ab4147. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in a tested species and application, we would like to follow up on t...

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NUMB antibody

 Inconclusive
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Skin equivalent)
Specification
Skin equivalent
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako EnVision Kit 5007
Permeabilization
No
Blocking step
(agent) for 20 minute(s) · Concentration: 100% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Jul 13 2012

Immunocytochemistry/ Immunofluorescence abreview for Anti-NUMB antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (skin equivalent, human keratinocytes Wholemount)
Specification
skin equivalent, human keratinocytes Wholemount
Fixative
Formaldehyde
Permeabilization
Yes - 0.2% Triton-X100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Jul 06 2012

Dear Tech support

Could you pleasespeculate regarding ab4147 reactivity against Drosophila derived samples?

Would you like to offer a participation in the abreview promotion for Drosophila with one of the Numb antibodies available...

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Thank you for contacting us.


The anti-NUMB antibody (ab4147) is raised against a peptide corresponding to residues 638-651 of human NUMB. Through sequence alignment of the Drosophila melanogaster protein (SwissProt reference http://www....

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Immunocytochemistry/ Immunofluorescence abreview for Anti-NUMB antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (embryonic brain tissue)
Specification
embryonic brain tissue
Fixative
Paraformaldehyde
Permeabilization
Yes - 1% TX100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Username

Abcam user community

Verified customer

提交于 Dec 09 2009

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NUMB antibody

 Poor
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (embryo CNS)
Specification
embryo CNS
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM sodiumcitrate pH 6.0, also Tris-EDTA pH 9.0 tried
Permeabilization
Yes - 0.1 % Tween-20 for 10-30 minutes
Blocking step
!% BSA and 10%donkey serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Oct 26 2007

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NUMB antibody

 Below Average
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (embryo)
Specification
embryo
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6%
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Username

Abcam user community

Verified customer

提交于 Jul 13 2007

Western blot abreview for Anti-NUMB antibody

 Average
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (embryo brain)
Loading amount
30 µg
Specification
embryo brain
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Jun 05 2007

BATCH NUMBER 141307 ORDER NUMBER 96008 DESCRIPTION OF THE PROBLEM Wrong band size: band size is supposed to be approx 71kDa, but I am getting a very robust band at about 115kDa that is much stronger than the band I see at about 70kDa...

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Thank you for your enquiry and for providing me with details of the western blotting experiments that you have performed. From the internal notes that I have I can see that mouse reactivity by this antibody was added as a result of two independent A...

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