Anti-NUMB抗体(ab4147)
Key features and details
- Goat polyclonal to NUMB
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-NUMB抗体
参阅全部 NUMB 一抗 -
描述
山羊多克隆抗体to NUMB -
宿主
Goat -
特异性
This antibody is expected to recognize all reported isoforms (NP_001005743.1, 001005744.1, NP_003735, NP_001005745.1). It is also likely to cross-react with Numblike (NUMBL, GeneID: 9253) which has a very similar C-Terminus, although this has not yet been tested experimentally. -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Chicken, Cow, Dog
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免疫原
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阳性对照
- WB: Mouse brain lysate; ICC: A431 and NIH3T3 cells; IHC-P: Human prostate tissue.
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常规说明
This product may cross-react with Numblike (NUMBL, GeneID: 9253) Principal Names - NUMB; S171; numb homolog (Drosophila) Official Gene Symbol - NUMB GenBank Accession Number – NP_003735 LocusLink ID - 8650 (human); 18222 (mouse) Gene Ontology terms - integral to plasma membraneReproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab4147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 71 kDa). 1 hour primary incubation at room temperature is recommended for this product. |
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| ICC/IF | Use a concentration of 10 µg/ml. |
靶标
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功能
Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. May also mediate local repair of brain ventricular wall damage. -
序列相似性
Contains 1 PID domain. -
翻译后修饰
Isoform 1 and isoform 2 are ubiquitinated by LNX leading to their subsequent proteasomal degradation (By similarity). Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 512187 Cow
- Entrez Gene: 480381 Dog
- Entrez Gene: 8650 Human
- Entrez Gene: 18222 Mouse
- Omim: 603728 Human
- SwissProt: P49757 Human
- SwissProt: Q9QZS3 Mouse
- Unigene: 654609 Human
see all -
别名
- c14_5527 antibody
- C14orf41 antibody
- FLJ31314 antibody
see all
图片
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Western blot - Anti-NUMB antibody (ab4147)Anti-NUMB antibody (ab4147) at 1 µg/ml + Mouse brain lysates at 35 µg
Predicted band size: 71 kDaDetected by chemiluminescence.
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Immunocytochemistry/ Immunofluorescence - Anti-NUMB antibody (ab4147)Immunocytochemistry/ Immunofluorescence analysis of A431 cells labeling NUMB with ab4147 at 10ug/mL. Alexa Fluor488 at 2ug/mL was used as the secondary antibody. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/mL) followed by Alexa Fluor488 secondary antibody (2ug/mL).Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. -
Immunocytochemistry/ Immunofluorescence - Anti-NUMB antibody (ab4147)Immunocytochemistry/ Immunofluorescence analysis of NIH3T3 cells labeling NUMB with ab4147 at 10ug/mL. Alexa Fluor488 at 2ug/mL was used as the secondary antibody. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/mL) followed by Alexa Fluor488 secondary antibody (2ug/mL).
Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton.
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Immunocytochemistry/ Immunofluorescence - Anti-NUMB antibody (ab4147)This image is courtesy of an anonymous Abreviewab4147 staining NUMB in the NIH3T3 cell line from Mouse fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 10% serum for 60 minutes at 24°C. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit polyclonal(1/500) was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUMB antibody (ab4147)Single collagenase-isolated mouse muscle fibre stained with DAPI (blue) to reveal nuclei and anti-Numb (green) which detects satellite cells.
This picture was kindly supplied as part of the review submitted by Christopher Mann.
文献 (32)
ab4147 被引用在 32 文献中.
- Li Y et al. CUL4B contributes to cancer stemness by repressing tumor suppressor miR34a in colorectal cancer. Oncogenesis 9:20 (2020). PubMed: 32054830
- Zhao Y et al. CD82-TRPM7-Numb signaling mediates age-related cognitive impairment. Geroscience 42:595-611 (2020). PubMed: 32088828
- Huang G et al. TUSC7 suppression of Notch activation through sponging MiR-146 recapitulated the asymmetric cell division in lung adenocarcinoma stem cells. Life Sci 232:116630 (2019). PubMed: 31279783
- Abdi K et al. EGFR Signaling Termination via Numb Trafficking in Ependymal Progenitors Controls Postnatal Neurogenic Niche Differentiation. Cell Rep 28:2012-2022.e4 (2019). PubMed: 31433979
- Cheng Y et al. m6A RNA Methylation Maintains Hematopoietic Stem Cell Identity and Symmetric Commitment. Cell Rep 28:1703-1716.e6 (2019). PubMed: 31412241
