Anti-NuMA抗体(ab97585)
Key features and details
- Rabbit polyclonal to NuMA
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-NuMA抗体
参阅全部 NuMA 一抗 -
描述
兔多克隆抗体to NuMA -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant protein fragment corresponding to a region within amino acids 1 and 309 of Human NuMA (AAH04165).
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阳性对照
- 293T, A431, H1299, HeLa, MOLT4 or Raji cell lines.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97585于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 238 kDa.
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IHC-P | (1) |
1/100 - 1/500.
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ICC/IF | (1) |
1/100 - 1/200.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 238 kDa. |
IHC-P
1/100 - 1/500. |
ICC/IF
1/100 - 1/200. |
靶标
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功能
May be a structural component of the nucleus. -
细胞定位
Nucleus. Chromosome. Dissociates from condensing chromosomes during early prophase, before the complete disintegration of the nuclear lamina. As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident. - Information by UniProt
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数据库链接
- Entrez Gene: 4926 Human
- Omim: 164009 Human
- SwissProt: Q14980 Human
- Unigene: 325978 Human
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别名
- Centrophilin stabilizes mitotic spindle in mitotic cells antibody
- NMP 22 antibody
- Nuclear matrix protein 22 antibody
see all
图片
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ab97585, at 1/200, staining paraformaldehyde-fixed A431 cells by Immunofluorescence (Panel 1) and merged with DNA probe (Panel 2).
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Anti-NuMA antibody (ab97585) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 238 kDa
7.5% SDS PAGE -
ab97585, at 1/100, staining paraffin-embedded Cal27 Xenograft by Immunohistochemistry.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (20)
ab97585 被引用在 20 文献中.
- Sun M et al. NuMA regulates mitotic spindle assembly, structural dynamics and function via phase separation. Nat Commun 12:7157 (2021). PubMed: 34887424
- Almstedt E et al. Integrative discovery of treatments for high-risk neuroblastoma. Nat Commun 11:71 (2020). PubMed: 31900415
- Michailidis E et al. Expansion, in vivo-ex vivo cycling, and genetic manipulation of primary human hepatocytes. Proc Natl Acad Sci U S A 117:1678-1688 (2020). PubMed: 31915293
- Mao Y et al. Stem-like tumor cells involved in heterogeneous vasculogenesis in breast cancer. Endocr Relat Cancer 27:23-39 (2020). PubMed: 31705798
- Yip JLK et al. AGS3 and Gai3 Are Concomitantly Upregulated as Part of the Spindle Orientation Complex during Differentiation of Human Neural Progenitor Cells. Molecules 25:N/A (2020). PubMed: 33172018