The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500.
May be a structural component of the nucleus.
Nucleus. Chromosome. Dissociates from condensing chromosomes during early prophase, before the complete disintegration of the nuclear lamina. As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident.
Centrophilin stabilizes mitotic spindle in mitotic cells antibody
NMP 22 antibody
Nuclear matrix protein 22 antibody
Nuclear mitotic apparatus protein 1 antibody
Nuclear mitotic apparatus protein antibody
NUMA 1 antibody
NuMA protein antibody
SP H antigen antibody
SP-H antigen antibody
Structural nuclear protein antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NuMA antibody (ab84680)This image is courtesy of an anonymous Abreview
ab84680 staining NuMA in Marmoset (common) testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with Buoin's fixative, permeabilized in Triton X-100 (1/100 in PBS) and blocked with 25% serum for 30 minutes; antigen retrieval was by heat mediation in a citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/100 in PBS + 0.5% BSA) for 17 hours at 4°C. A Biotin-conjugated Chicken anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.
ab84680, at 1/100 dilution, staining NuMA in formalin-fixed, paraffin-embedded human breast carcinoma by Immunohistochemistry using a Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Hilyte PlusTM 555 secondary antibody at a dilution of 1/100.