Anti-NuMA抗体(ab84680)
Key features and details
- Rabbit polyclonal to NuMA
- Suitable for: IHC-P
- Reacts with: Human, Common marmoset
- Isotype: IgG
概述
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产品名称
Anti-NuMA抗体
参阅全部 NuMA 一抗 -
描述
兔多克隆抗体to NuMA -
宿主
Rabbit -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human, Common marmoset
预测可用于: Chimpanzee, Gorilla -
免疫原
Synthetic peptide corresponding to a region between residue 900 and 950 of Human NuMA (NP_006176.2).
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阳性对照
- IHC-P: Human breast carcinoma, seminoma and ovarian tumor tissue. Common marmoset testis tissue.
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常规说明
Concentration is optimized for IHC and not determined
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab84680于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P | (1) |
1/100 - 1/500.
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说明 |
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IHC-P
1/100 - 1/500. |
靶标
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功能
May be a structural component of the nucleus. -
细胞定位
Nucleus. Chromosome. Dissociates from condensing chromosomes during early prophase, before the complete disintegration of the nuclear lamina. As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident. - Information by UniProt
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数据库链接
- Entrez Gene: 4926 Human
- Omim: 164009 Human
- SwissProt: Q14980 Human
- Unigene: 325978 Human
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别名
- Centrophilin stabilizes mitotic spindle in mitotic cells antibody
- NMP 22 antibody
- Nuclear matrix protein 22 antibody
see all
图片
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ab84680 staining NuMA in Marmoset (common) testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with Buoin's fixative, permeabilized in Triton X-100 (1/100 in PBS) and blocked with 25% serum for 30 minutes; antigen retrieval was by heat mediation in a citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/100 in PBS + 0.5% BSA) for 17 hours at 4°C. A Biotin-conjugated Chicken anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.
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ab84680, at 1/100 dilution, staining NuMA in formalin-fixed, paraffin-embedded human breast carcinoma by Immunohistochemistry using a Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Hilyte PlusTM 555 secondary antibody at a dilution of 1/100.
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ab84680, at 1/250 dilution, staining NuMA in formalin-fixed, paraffin-embedded human breast carcinoma by Immunohistochemistry using DAB staining.
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ab84680, at 1/250 dilution, staining NuMA in formalin-fixed, paraffin-embedded human seminoma by Immunohistochemistry using DAB staining.
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ab84680, at 1/250 dilution, staining NuMA in formalin-fixed, paraffin-embedded human ovarian tumor by Immunohistochemistry using DAB staining.
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab84680 被引用在 10 文献中.
- Liu X et al. 3D heterospecies spheroids of pancreatic stroma and cancer cells demonstrate key phenotypes of pancreatic ductal adenocarcinoma. Transl Oncol 14:101107 (2021). PubMed: 33946033
- Bayerl J et al. Principles of signaling pathway modulation for enhancing human naive pluripotency induction. Cell Stem Cell 28:1549-1565.e12 (2021). PubMed: 33915080
- Petrus-Reurer S et al. Preclinical safety studies of human embryonic stem cell-derived retinal pigment epithelial cells for the treatment of age-related macular degeneration. Stem Cells Transl Med 9:936-953 (2020). PubMed: 32319201
- Dimitracopoulos A et al. Mechanochemical Crosstalk Produces Cell-Intrinsic Patterning of the Cortex to Orient the Mitotic Spindle. Curr Biol 30:3687-3696.e4 (2020). PubMed: 32735816
- Bendriem RM et al. Tight junction protein occludin regulates progenitor Self-Renewal and survival in developing cortex. Elife 8:N/A (2019). PubMed: 31794381
- Lu R et al. Neurons generated from carcinoma stem cells support cancer progression. Signal Transduct Target Ther 2:16036 (2017). PubMed: 29263908
- Huang CW et al. The Differentiation Stage of Transplanted Stem Cells Modulates Nerve Regeneration. Sci Rep 7:17401 (2017). PubMed: 29234013
- Shangguan W et al. Endothelium originated from colorectal cancer stem cells constitute cancer blood vessels. Cancer Sci 108:1357-1367 (2017). IHC-Fr . PubMed: 28421697
- Petrus-Reurer S et al. Integration of Subretinal Suspension Transplants of Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cells in a Large-Eyed Model of Geographic Atrophy. Invest Ophthalmol Vis Sci 58:1314-1322 (2017). PubMed: 28241319
- Sugawa F et al. Human primordial germ cell commitment in vitro associates with a unique PRDM14 expression profile. EMBO J 34:1009-24 (2015). IHC-P ; Human . PubMed: 25750208