为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

我们使用cookies,使我们网站尽可能对您有帮助。

我们的Cookie政策介绍了您如何能够取消cookie使用。

如果您不改变cookie设置,我们认为您愿意接受cookie。

继续 继续

致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 诊断和治疗的解决方案
    定制方案及合作关系

    定制抗体开发及商务合作助力您的诊断和治疗探索。

    与我们一起开创定制方案

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Cell Biology Cell Cycle Markers

Anti-NuMA抗体[A73-B/D12] (ab5675)

  • Datasheet
  • SDS
Reviews (1)Q&A (6)References (6)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Key features and details

  • Mouse monoclonal [A73-B/D12] to NuMA
  • Reacts with: Human
  • Isotype: IgM

你可能会感兴趣

蛋白
Product image
Recombinant Human NuMA protein (ab114324)

查看更多相关产品

概述

  • 产品名称

    Anti-NuMA抗体[A73-B/D12]
    参阅全部 NuMA 一抗
  • 描述

    小鼠单克隆抗体[A73-B/D12] to NuMA
  • 宿主

    Mouse
  • 特异性

    Reacts with an intracellular 228 kD protein found in the nucleus during interphase.
  • 种属反应性

    与反应: Human
  • 免疫原

    Live Ls 174T cells (human colon carcinoma).

  • 阳性对照

    • Tonsil, Thymus, Spleen.
  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • 存储溶液

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度

    Protein G purified
  • 克隆

    单克隆
  • 克隆编号

    A73-B/D12
  • 同种型

    IgM
  • 研究领域

    • Cell Biology
    • Cell Cycle
    • Markers
    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Spindle

相关产品

  • Alternative Versions

    • Anti-NuMA antibody [A73-B/D12] - BSA and Azide free (ab212688)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
  • Isotype control

    • Mouse IgM [B11/7] - Isotype control (ab91545)
  • Recombinant Protein

    • Recombinant Human NuMA protein (ab114324)

靶标

  • 功能

    May be a structural component of the nucleus.
  • 细胞定位

    Nucleus. Chromosome. Dissociates from condensing chromosomes during early prophase, before the complete disintegration of the nuclear lamina. As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident.
  • Target information above from: UniProt accession Q14980 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 4926 Human
    • Omim: 164009 Human
    • SwissProt: Q14980 Human
    • Unigene: 325978 Human
    • 别名

      • Centrophilin stabilizes mitotic spindle in mitotic cells antibody
      • NMP 22 antibody
      • Nuclear matrix protein 22 antibody
      • Nuclear mitotic apparatus protein 1 antibody
      • Nuclear mitotic apparatus protein antibody
      • NUMA 1 antibody
      • NUMA antibody
      • NuMA protein antibody
      • NUMA1 antibody
      • NUMA1_HUMAN antibody
      • SP H antigen antibody
      • SP-H antigen antibody
      • Structural nuclear protein antibody
      see all

    实验方案

    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet
    • SDS
  • 文献 (6)

    发表研究结果有使用 ab5675?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab5675 被引用在 6 文献中.

    • Abd-Elrahman KS  et al. Autophagy is increased following either pharmacological or genetic silencing of mGluR5 signaling in Alzheimer's disease mouse models. Mol Brain 11:19 (2018). PubMed: 29631635
    • Burch A  et al. A novel synaptic junction preparation for the identification and characterization of cleft proteins. PLoS One 12:e0174895 (2017). PubMed: 28362857
    • Goniotaki D  et al. Inhibition of group-I metabotropic glutamate receptors protects against prion toxicity. PLoS Pathog 13:e1006733 (2017). PubMed: 29176838
    • Han K  et al. SHANK3 overexpression causes manic-like behaviour with unique pharmacogenetic properties. Nature 503:72-7 (2013). PubMed: 24153177
    • Shi JG  et al. Tissue engineering of ureteral grafts by seeding urothelial differentiated hADSCs onto biodegradable ureteral scaffolds. J Biomed Mater Res A 100:2612-22 (2012). ICC/IF . PubMed: 22615210
    • Kwong J  et al. An antagonist of the chemokine receptor CXCR4 induces mitotic catastrophe in ovarian cancer cells. Mol Cancer Ther 8:1893-905 (2009). PubMed: 19567818

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-7 of 7 Abreviews or Q&A

    Question

    In order to choose the best NuMA antibody for this application, I need to know the epitope-recognition sites. For Ab86129, you only indicate a range (a.a. 900 – 1000) and for Ab5675, I could not find this information. Can you disclose the epitope-recognition sites for both antibodies?

    Also, could you provide Ab86129 and/or Ab84680 without BSA? If yes, at what cost?

    Read More

    Abcam community

    Verified customer

    Asked on Sep 11 2012

    Answer

    Thanks for your enquiry.

    Regarding the immunogen information:
    ab5675 immunogen is Live Ls 174T cells and epitope is not known.

    RE: could you provide Ab86129 and/or Ab84680 without BSA?
    These 2 antibodies are not typically available in a formulation without BSA. There are 2 alternatives to remove the BSA.

    1. For bulk orders we can typically provide you with a custom formulation. A bulk order would require purchase of at least 10 vials of antibody. Bulk orders are provided at a discount. For further information regarding bulk orders please contact Andrea Gray at mailto:Andrea.Gray@abcam.com.

    2. We have a protein purification kit that would allow you to remove the BSA containing buffer provided with the antibody and replace it with a buffer of your choosing. Please see our product https://www.abcam.com/Antibody-Purification-Kit-Protein-A-ab102784.htmlfor more details.

    I hope this is helpful. Please contact me again if you have any further questions.

    Read More

    Abcam Scientific Support

    回复于 Sep 11 2012

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NuMA antibody [A73-B/D12]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Ovarian carcinoma)
    Specification
    Ovarian carcinoma
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid pH6
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
    Read More

    Mr. Carl Hobbs

    Verified customer

    提交于 Mar 16 2009

    Question

    Thank you for your reply. I'd like to try a new vial, if it's possible. And about permeability, I am particulary interested in looking at colocalization of NuMA with our target protein, Mx during metaphase. During metaphase NuMA is supposed to localize near the minus end of mitotic spindles (polar ends), which localize in the cytoplasm after the nuclear membrane breakdown. Mx protein is the cytoplasmic protein which I beatifully stain all the time in the cells. Also I sucesfully stained beta, gamma tubulin (form your company) in the cells. I could not even see the expected staing pattern of NuMA during metaphase. Therefore, I believe that permiablity is not the problem in my case. Again, I am interested in using a new vial to try again but if it doesn't work again (probably it won't),I would like to have a refund. Thank you for your time and suggestion.

    Read More

    Abcam community

    Verified customer

    Asked on Apr 13 2005

    Answer

    Thank you for your e-mail. This is to let you know that we have placed a new order for you one vial of ab5675 as a replacement vial from a new batch. For your information, the new order number is 80286. We hope this new vial will work. Please do let me know how you are getting on.

    Read More

    Abcam Scientific Support

    回复于 Apr 22 2005

    Question

    I do not think your advice is helpful. First of all, using higher concentration of NuMA antibody is not good idea because I already observed very strong background when I usd 1:100 dilution of NuMA. Sounds like if this NuMA antibody can not even recognize the antigen (Hela) from the same species (human colon carcinoma), there is no way that NuMA antibody can recognize the sheep cell line. Ant yet, many other researchers showed that mouse mono-clonal NuMA antibody (from other company)clearly recognize antigens in Hela cells. I just want to have refund because I don't have any good reasons from you that I should try again. I would like to purchase the NuMA antibody from other company in stead. I just don't want you to misunderstand this. I really like products from Abcam. Other antibody worked great, just not NuMA. I am tyring to finish up my Ph.D program so I don't have time to get that posititive control you sugesst to find out that your NuMA is not crosreactive with sheep.

    Read More

    Abcam community

    Verified customer

    Asked on Apr 08 2005

    Answer

    Thank you for your reply. We are very sorry that you are not satisfied with our response. This antibody has been tested and characterized for IHC using human colon carcinoma. Unfortunately, we have no information if this product reacts with HeLa cells, although probably it should do. The staining is nuclear and cytoplasmic. Since it is a nuclear antibody, it may be necessary to permeabilize the cells before staining. Looking at our order and stock data, we have sold several vials from the same Lot and we have not received any complaint about this antibody so far. Previously, this antibody is being tried on ovine glandular epithelium fixed with paraformldehyde (PFA). Normally PFA treated cells or tissues give autoflorescence. If you want to try again, we can send you a new vial for testing or if you would rather get a refund the money, we can arrange it for you. Please do let us know how you would prefer to proceed.

    Read More

    Abcam Scientific Support

    回复于 Apr 12 2005

    Question

    BATCH NUMBER 92289 DESCRIPTION OF THE PROBLEM Non-specific staining, I did not see any NuMA staining pattern I was supposed to see. For example, NuMA was supposed to be in the nucleus curing interphase, which I did not see. SAMPLE Sheep/ ovine uterine derived cell line (ovine glandular epithelium (oGE)cells) PRIMARY ANTIBODY Abcam. Anti mouse monoclonal to NuMA. Cells wre incubated with NuMA antibody RT for 2 hours. I used several dilution starting with 1: 100, 1:500, or 1: 1000 in 2%BSA-1XPBS. Cells were washed with three times for 5 min on the rocker before and after primary antibody treatment. None of them worked. SECONDARY ANTIBODY Molecular probe. I used goat anti- mouse IgM antibody(Alexa Fluor 555) with the dilution of 1:1000 or 1: 2000. None of them worked. 2%BSA-1XPBS was used as diluent. Cells were incubated with this secondary antibody for 1 hour at RT. DETECTION METHOD Absorbance of this secondary antibody was 555, which gives us red color. POSITIVE AND NEGATIVE CONTROLS USED Hela cells were used as a positive control because NuMA antobody was against human colon carcinoma. The setting of this assay was the sama as staining with oGE cell. Again, it did not work. Staining pattern was very similar to oGE cells. ANTIBODY STORAGE CONDITIONS 4C FIXATION OF SAMPLE Cells were fixed with 3% Paraformaldehyde at RT for 10 min. BLOCKING CONDITIONS Cells were blocked with Base solution including 1XPBS, 0.05% TritonX-100, and 2%BSA at 4C for overnight. HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? I used several concentrations of NuMA as well as sencondary antibody. ADDITIONAL NOTES I sent this enquiry 2 month ago but I did not get any emails back. I would like to have either a new patch which you confirm the sensitivity or refund. We from Dr Troy Ott' lab at University of Idaho purchased many antiboddies from your comany but NuMA is the only one that did not work. Other antibodies worked beautifully. We'd like to still continue to use your company's products because of the reliability, however, I do not think your technical help is very supportive. Please give us some suggestions or slutions. Thank you.

    Read More

    Abcam community

    Verified customer

    Asked on Apr 05 2005

    Answer

    Thank you for your enquiry. We are very sorry to hear that you are having problem with this antibody. We have looked at our computer system which stores all the technical enquiries, complaints and correspondence. Unfortunately, we have not received any previous message from you and this is your first e-mail which reached Abcam. We would like to draw your attention to the fact that this antibody has not bee tested for cross-reaction with sheep/ovine therefore we do not know if it recognizes this species at all. The immunogen used to raise this antibody is Live Ls 174T cells (human colon carcinoma). It is true that ab5675 recognizes human NuMA. However, HeLa cells are not human colon carcinoma cell line but human cervix carcinoma cell line. Good positive controls for this antibody are human tonsil, thymus, spleen tissue sections. We would strongly suggest using positive control along with the samples to make sure that the detection system works properly and the experimental conditions are sufficient. We would also advise applying higher concentration of this primary antibody i.e. 1/50. We hope this information will be useful for you. Should you still have problem with this antibody, then please do not hesitate to contact us again.

    Read More

    Abcam Scientific Support

    回复于 Apr 08 2005

    Question

    Would you have pictures of Immunofluorescence using this antibody on human cells ?

    Read More

    Abcam community

    Verified customer

    Asked on Jan 07 2005

    Answer

    Thank you for your enquiry. We unfortunately do not have an image available for ab5675. The antibody has been tested for application in Western blot, Immunohistochemistry (Formalin-fixed paraffin-embedded sections), and Immunohistochemistry (Frozen sections), but has not to our knowledge been tested in Immunocytochemistry or IF. If you have any additional questions, please contact us again.

    Read More

    Abcam Scientific Support

    回复于 Jan 07 2005

    Question

    We received item ab5675 and noticed the color was red.... A few weeks ago a customer received item ab3076 that was also red. You sent me an e-mail stating item ab3076 should not be red. My question is item ab5675 suppose to be red??? Also we are still waiting for item ab3076 replacement can you give me lead time for replacement item ab3076?? Please advise...

    Read More

    Abcam community

    Verified customer

    Asked on May 14 2004

    Answer

    Thank you so much for your patience. I had to contact the originator of ab5675 and have just heard back. Concerning ab5675, the antibody is red in color because it is concentrated medium from cell culture containing phenol red. The other antibody, ab3076, was not supposed to be red and your replacement was on order#41653 I believe. If you have any more questions or concerns please do contact us again.

    Read More

    Abcam Scientific Support

    回复于 May 25 2004

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    沪ICP备14046373号-1

    沪公网安备 31011502007413号

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2021 艾博抗(上海)贸易有限公司版权所有