Anti-Nucleoporin p62/NUP62抗体(ab96134)
Key features and details
- Rabbit polyclonal to Nucleoporin p62/NUP62
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-Nucleoporin p62/NUP62抗体
参阅全部 Nucleoporin p62/NUP62 一抗 -
描述
兔多克隆抗体to Nucleoporin p62/NUP62 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Cow, Zebrafish, Rhesus monkey -
免疫原
Recombinant fragment corresponding to Human Nucleoporin p62/NUP62 aa 294-502.
Database link: NP_057637 -
阳性对照
- WB: A431, H1299, PC-12, Rat2, Neuro-2a, GL261, C8D30, NIH/3T3, BCL-1, RAW 264.7 and C2C12 whole cell lysate. ICC/IF: A431 cells. IHC-P: Mouse fore brain tissue.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96134于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/100 - 1/1000.
|
|
WB | (2) |
1/500 - 1/3000. Predicted molecular weight: 53 kDa.
|
ICC/IF | (1) |
1/100 - 1/200.
|
说明 |
---|
IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 53 kDa. |
ICC/IF
1/100 - 1/200. |
靶标
-
相关性
The nuclear pore complex is a structure that extends across the nuclear envelope and regulates the flow of macromolecules between the cytoplasm and the nucleus. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. Nup 62 is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. There are multiple transcript variants of this gene, however, they encode a single protein isoform. This protein undergoes post-translational modifications. It contains about 10 N-acetylglucosamine side chain sites. -
细胞定位
Nucleus; nuclear pore complex. Cytoplasm; cytoskeleton; spindle pole. Note: Central region of the nuclear pore, within the transporter. During mitotic cell division, it associates with the poles of the mitotic spindle. -
数据库链接
- Entrez Gene: 23636 Human
- Entrez Gene: 18226 Mouse
- Entrez Gene: 65274 Rat
- Entrez Gene: 100426308 Rhesus monkey
- Omim: 605815 Human
- SwissProt: P37198 Human
- SwissProt: Q63850 Mouse
- SwissProt: P17955 Rat
-
别名
- 62 kDa nucleoporin antibody
- DKFZp547L134 antibody
- FLJ20822 antibody
see all
图片
-
All lanes : Anti-Nucleoporin p62/NUP62 antibody (ab96134) at 1/1000 dilution
Lane 1 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate
Lane 2 : GL261 whole cell lysate
Lane 3 : C8D30 whole cell lysate
Lane 4 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 5 : BCL-1 (mouse B lymphocyte lymphoma cell line) whole cell lysate
Lane 6 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 7 : C2C12 (Mouse myoblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 53 kDa10% SDS-PAGE.
-
ab96134, at a 1/200 dilution, staining Nucleoporin p62/NUP62 in paraformaldehyde fixed A431 by Immunofluorescence analysis.
Lower image: merged with DNA probe. -
All lanes : Anti-Nucleoporin p62/NUP62 antibody (ab96134) at 1/1000 dilution
Lane 1 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 2 : Rat2 (Rat fibroblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 53 kDa10% SDS-PAGE.
-
Paraffin-embedded mouse fore brain tissue stained for Nucleoporin p62/NUP62 using ab96134 at 1/500 dilution in immunohistochemical analysis.
-
All lanes : Anti-Nucleoporin p62/NUP62 antibody (ab96134) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 53 kDa
10% SDS PAGE -
Immunofluorescence analysis of A431 cells, staining Nucleoporin p62/NUP62 with ab96134 at 1/200 dilution, demonstrating punctate nuclear staining.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (9)
ab96134 被引用在 9 文献中.
- Zhao Z et al. miRNA profiling of primate cervicovaginal lavage and extracellular vesicles reveals miR-186-5p as a potential antiretroviral factor in macrophages. FEBS Open Bio 10:2021-2039 (2020). PubMed: 33017084
- Li W et al. miR-129-5p attenuates hypoxia-induced apoptosis in rat H9c2 cardiomyocytes by activating autophagy. J Gene Med 22:e3200 (2020). PubMed: 32298509
- Qiao J et al. Inhibition of HIF-1a restrains fracture healing via regulation of autophagy in a rat model. Exp Ther Med 17:1884-1890 (2019). PubMed: 30783464
- Clift D et al. A Method for the Acute and Rapid Degradation of Endogenous Proteins. Cell 171:1692-1706.e18 (2017). PubMed: 29153837
- Ouyang F et al. HMGB1 induces apoptosis and EMT in association with increased autophagy following H/R injury in cardiomyocytes. Int J Mol Med 37:679-89 (2016). WB . PubMed: 26847839
- Lund PJ et al. Global Analysis of O-GlcNAc Glycoproteins in Activated Human T Cells. J Immunol 197:3086-3098 (2016). Human . PubMed: 27655845
- Guan JJ et al. DRAM1 regulates apoptosis through increasing protein levels and lysosomal localization of BAX. Cell Death Dis 6:e1624 (2015). PubMed: 25633293
- Tojo Y et al. Hypoxia Signaling Cascade for Erythropoietin Production in Hepatocytes. Mol Cell Biol 35:2658-72 (2015). Mouse . PubMed: 26012551
- Isnard A et al. Impact of Leishmania infection on host macrophage nuclear physiology and nucleopore complex integrity. PLoS Pathog 11:e1004776 (2015). WB . PubMed: 25826301