Application
Western blot
Sample
Mouse Cell lysate - nuclear (C17.2 mouse neural precursor cell line)
Loading amount
25 µg
Specification
C17.2 mouse neural precursor cell line
Gel Running Conditions
Reduced Denaturing (10% Novex Bis Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS/T
Secondary antibody
Name
Non-Abcam antibody was used: LiCOR IRDye
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Dilution
1/10000
Detection
Detection method
Fluorescence scanning
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 38 kDa Non-specific: 36 (faint) kDa
Additional data
Additional Notes
Lane 1: Marker
Lanes 2 -4 : Crude (slow spin) nuclear fractions of C17.2 cells
PVDF membrane
Lanes 2 -4 : Crude (slow spin) nuclear fractions of C17.2 cells
PVDF membrane
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Claire Thornton
Verified customer
提交于 Aug 30 2012